Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples
Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered from consecutive sputum samples collected from a CF patient and...
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| Format: | Article |
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Society for General Microbiology
2019
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| _version_ | 1825722071744774144 |
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| author | See-Too, Wah Seng Ambrose, Mark Malley, Roslyn Ee, Robson Mulcahy, Emily Manche, Emily Lazenby, James McEwan, Belinda Pagnon, Joanne Chen, Jian Woon Chan, Kok Gan Turnbull, Lynne Whitchurch, Cynthia B. Roddam, Louise F. |
| author_facet | See-Too, Wah Seng Ambrose, Mark Malley, Roslyn Ee, Robson Mulcahy, Emily Manche, Emily Lazenby, James McEwan, Belinda Pagnon, Joanne Chen, Jian Woon Chan, Kok Gan Turnbull, Lynne Whitchurch, Cynthia B. Roddam, Louise F. |
| author_sort | See-Too, Wah Seng |
| collection | UM |
| description | Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered from consecutive sputum samples collected from a CF patient and identified as Pandoraea pnomenusa underwent a polyphasic taxonomic analysis. The isolates were found to be Gram-negative, facultative anaerobic motile bacilli and subsequently designated as strains 6399 T (=LMG29626 T =DSM103228 T ) and 7641 (=LMG29627=DSM103229), respectively. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that 6399 T and 7641 formed a distinct phylogenetic lineage within the genus Pandoraea. Genome sequence comparison analysis indicated that strains 6399 T and 7641 are clonal and share 100 % similarity, however, similarity to other type strains (ANIb 73.2–88.8 %, ANIm 83.5–89.9 % and OrthoANI 83.2– 89.3 %) indicates that 6399 T and 7641 do not belong to any of the reported type species. The major cellular fatty acids of 6399 T were C 16: 0 (32.1 %) C 17: 0 cyclo (18.7 %) and C 18: 1 !7c (14.5 %), while Q-8 was the only respiratory quinone detected. The major polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of 6399 T was 62.9 (mol%). Strain 6399 T can be differentiated from other members of Pandoraea by the absence of C 19: 0 !8c cyclo and by the presence of C 17: 0 !8c cyclo. Together our data show that the bacterial strains 6399 T and 7641 represent a novel species of the genus Pandoraea, for which the name Pandoraea fibrosis sp. nov. is proposed (type strain 6399 T ). © 2019 IUMS. |
| first_indexed | 2024-03-06T06:00:03Z |
| format | Article |
| id | um.eprints-23504 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:00:03Z |
| publishDate | 2019 |
| publisher | Society for General Microbiology |
| record_format | dspace |
| spelling | um.eprints-235042020-01-21T06:38:24Z http://eprints.um.edu.my/23504/ Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples See-Too, Wah Seng Ambrose, Mark Malley, Roslyn Ee, Robson Mulcahy, Emily Manche, Emily Lazenby, James McEwan, Belinda Pagnon, Joanne Chen, Jian Woon Chan, Kok Gan Turnbull, Lynne Whitchurch, Cynthia B. Roddam, Louise F. Q Science (General) QR Microbiology R Medicine Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered from consecutive sputum samples collected from a CF patient and identified as Pandoraea pnomenusa underwent a polyphasic taxonomic analysis. The isolates were found to be Gram-negative, facultative anaerobic motile bacilli and subsequently designated as strains 6399 T (=LMG29626 T =DSM103228 T ) and 7641 (=LMG29627=DSM103229), respectively. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that 6399 T and 7641 formed a distinct phylogenetic lineage within the genus Pandoraea. Genome sequence comparison analysis indicated that strains 6399 T and 7641 are clonal and share 100 % similarity, however, similarity to other type strains (ANIb 73.2–88.8 %, ANIm 83.5–89.9 % and OrthoANI 83.2– 89.3 %) indicates that 6399 T and 7641 do not belong to any of the reported type species. The major cellular fatty acids of 6399 T were C 16: 0 (32.1 %) C 17: 0 cyclo (18.7 %) and C 18: 1 !7c (14.5 %), while Q-8 was the only respiratory quinone detected. The major polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of 6399 T was 62.9 (mol%). Strain 6399 T can be differentiated from other members of Pandoraea by the absence of C 19: 0 !8c cyclo and by the presence of C 17: 0 !8c cyclo. Together our data show that the bacterial strains 6399 T and 7641 represent a novel species of the genus Pandoraea, for which the name Pandoraea fibrosis sp. nov. is proposed (type strain 6399 T ). © 2019 IUMS. Society for General Microbiology 2019 Article PeerReviewed See-Too, Wah Seng and Ambrose, Mark and Malley, Roslyn and Ee, Robson and Mulcahy, Emily and Manche, Emily and Lazenby, James and McEwan, Belinda and Pagnon, Joanne and Chen, Jian Woon and Chan, Kok Gan and Turnbull, Lynne and Whitchurch, Cynthia B. and Roddam, Louise F. (2019) Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples. International Journal of Systematic and Evolutionary Microbiology, 69 (3). pp. 645-651. ISSN 1466-5026, DOI https://doi.org/10.1099/ijsem.0.003147 <https://doi.org/10.1099/ijsem.0.003147>. https://doi.org/10.1099/ijsem.0.003147 doi:10.1099/ijsem.0.003147 |
| spellingShingle | Q Science (General) QR Microbiology R Medicine See-Too, Wah Seng Ambrose, Mark Malley, Roslyn Ee, Robson Mulcahy, Emily Manche, Emily Lazenby, James McEwan, Belinda Pagnon, Joanne Chen, Jian Woon Chan, Kok Gan Turnbull, Lynne Whitchurch, Cynthia B. Roddam, Louise F. Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples |
| title | Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples |
| title_full | Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples |
| title_fullStr | Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples |
| title_full_unstemmed | Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples |
| title_short | Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples |
| title_sort | pandoraea fibrosis sp nov a novel pandoraea species isolated from clinical respiratory samples |
| topic | Q Science (General) QR Microbiology R Medicine |
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