Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism
This study reports on the evaluation of biodegradation and biosorption mechanism of indigenous bacteria on azo dye decolorization. The bacterial strains were screened for their ability in decolorizing of selected toxic azo dyes in aqueous solution. Lysinibacillus fusiformis W1B6 showed the highest d...
| Autori principali: | , |
|---|---|
| Natura: | Articolo |
| Pubblicazione: |
SAGE Publications
2019
|
| Soggetti: |
| _version_ | 1825722075768160256 |
|---|---|
| author | Sari, Ira Puspita Simarani, Khanom |
| author_facet | Sari, Ira Puspita Simarani, Khanom |
| author_sort | Sari, Ira Puspita |
| collection | UM |
| description | This study reports on the evaluation of biodegradation and biosorption mechanism of indigenous bacteria on azo dye decolorization. The bacterial strains were screened for their ability in decolorizing of selected toxic azo dyes in aqueous solution. Lysinibacillus fusiformis W1B6 showed the highest decolorizing of about 96% of methyl red within 2 h, with the specific growth rate of 0.273/h. The optimum decolorization under aerobic condition was achieved at pH 7.5, 30 ± 2°C, inoculum 10% (v/v), and initial dye concentration of 100 mg/L. Spectrum analysis confirmed biodegradation as the principal mechanism of decolorization, although biosorption also occurred. Extracellular and intracellular azoreductase, laccase, and lignin peroxidase plays a role in the oxidoreductive mechanism of decolorization. The absorption isotherms fitted with the Langmuir equation and the reaction kinetics of methyl red decolorization followed a pseudo first-order. © The Author(s) 2019. |
| first_indexed | 2024-03-06T06:00:07Z |
| format | Article |
| id | um.eprints-23525 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:00:07Z |
| publishDate | 2019 |
| publisher | SAGE Publications |
| record_format | dspace |
| spelling | um.eprints-235252020-01-22T02:31:47Z http://eprints.um.edu.my/23525/ Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism Sari, Ira Puspita Simarani, Khanom Q Science (General) QH Natural history QR Microbiology This study reports on the evaluation of biodegradation and biosorption mechanism of indigenous bacteria on azo dye decolorization. The bacterial strains were screened for their ability in decolorizing of selected toxic azo dyes in aqueous solution. Lysinibacillus fusiformis W1B6 showed the highest decolorizing of about 96% of methyl red within 2 h, with the specific growth rate of 0.273/h. The optimum decolorization under aerobic condition was achieved at pH 7.5, 30 ± 2°C, inoculum 10% (v/v), and initial dye concentration of 100 mg/L. Spectrum analysis confirmed biodegradation as the principal mechanism of decolorization, although biosorption also occurred. Extracellular and intracellular azoreductase, laccase, and lignin peroxidase plays a role in the oxidoreductive mechanism of decolorization. The absorption isotherms fitted with the Langmuir equation and the reaction kinetics of methyl red decolorization followed a pseudo first-order. © The Author(s) 2019. SAGE Publications 2019 Article PeerReviewed Sari, Ira Puspita and Simarani, Khanom (2019) Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism. Adsorption Science & Technology, 37 (5-6). pp. 492-508. ISSN 0263-6174, DOI https://doi.org/10.1177/0263617419848897 <https://doi.org/10.1177/0263617419848897>. https://doi.org/10.1177/0263617419848897 doi:10.1177/0263617419848897 |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Sari, Ira Puspita Simarani, Khanom Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism |
| title | Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism |
| title_full | Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism |
| title_fullStr | Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism |
| title_full_unstemmed | Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism |
| title_short | Decolorization of selected azo dye by Lysinibacillus fusiformis W1B6: Biodegradation optimization, isotherm, and kinetic study biosorption mechanism |
| title_sort | decolorization of selected azo dye by lysinibacillus fusiformis w1b6 biodegradation optimization isotherm and kinetic study biosorption mechanism |
| topic | Q Science (General) QH Natural history QR Microbiology |
| work_keys_str_mv | AT sariirapuspita decolorizationofselectedazodyebylysinibacillusfusiformisw1b6biodegradationoptimizationisothermandkineticstudybiosorptionmechanism AT simaranikhanom decolorizationofselectedazodyebylysinibacillusfusiformisw1b6biodegradationoptimizationisothermandkineticstudybiosorptionmechanism |