Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay
Despite the extensive work on anticancer drug discovery, the number of potent lead compounds that enter the preclinical and clinical trials thus far is still low due to the poor selectivity and understanding in pharmacodynamics. In view of the homology between zebrafish embryogenesis and carcinogene...
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| Format: | Article |
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SAGE Publications
2019
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| author | Rusdi, Nur 'Afina Kue, Chin Siang Yu, Ke Xin Lau, Beng Fye Chung, Lip Yong Kiew, Lik Voon |
| author_facet | Rusdi, Nur 'Afina Kue, Chin Siang Yu, Ke Xin Lau, Beng Fye Chung, Lip Yong Kiew, Lik Voon |
| author_sort | Rusdi, Nur 'Afina |
| collection | UM |
| description | Despite the extensive work on anticancer drug discovery, the number of potent lead compounds that enter the preclinical and clinical trials thus far is still low due to the poor selectivity and understanding in pharmacodynamics. In view of the homology between zebrafish embryogenesis and carcinogenesis in human, zebrafish embryos can be used in the screening platform to elucidate the molecular targets of potential anticancer compounds. In the present study, the possible targets modulating the potential anticancer effects of selected brown seaweed-derived compounds (ie alginate, fucoidan, phloroglucinol, fucosterol, and fucoxanthin) were examined. Teratogenic effects induced by the compounds were observed after 72 hours post-fertilization. Fucoidan, phloroglucinol, and fucosterol were observed to significantly reduce the pigmentation of the zebrafish in a dose-dependent manner at low concentrations (fucoidan, <60 µg/mL; phloroglucinol, <10 µg/mL; fucosterol, <3 µg/mL). On the other hand, embryos treated with fucoxanthin at 200 µg/mL and 300 µg/mL exhibited either phenotypes of curved trunk or bent tail. Further validation work using dual antiplatelet therapy (DAPT) and dorsomorphin as positive controls suggest that fucoxanthin might target the Notch and bone morphogenetic protein (BMP) pathways, respectively. Findings from this exploratory study henceforth have demonstrated the utility of zebrafish embryo to accelerate the discovery of potential compounds for targeted anticancer therapy. © The Author(s) 2019 |
| first_indexed | 2024-03-06T06:00:22Z |
| format | Article |
| id | um.eprints-23615 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:00:22Z |
| publishDate | 2019 |
| publisher | SAGE Publications |
| record_format | dspace |
| spelling | um.eprints-236152020-01-29T02:15:52Z http://eprints.um.edu.my/23615/ Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay Rusdi, Nur 'Afina Kue, Chin Siang Yu, Ke Xin Lau, Beng Fye Chung, Lip Yong Kiew, Lik Voon QR Microbiology R Medicine Despite the extensive work on anticancer drug discovery, the number of potent lead compounds that enter the preclinical and clinical trials thus far is still low due to the poor selectivity and understanding in pharmacodynamics. In view of the homology between zebrafish embryogenesis and carcinogenesis in human, zebrafish embryos can be used in the screening platform to elucidate the molecular targets of potential anticancer compounds. In the present study, the possible targets modulating the potential anticancer effects of selected brown seaweed-derived compounds (ie alginate, fucoidan, phloroglucinol, fucosterol, and fucoxanthin) were examined. Teratogenic effects induced by the compounds were observed after 72 hours post-fertilization. Fucoidan, phloroglucinol, and fucosterol were observed to significantly reduce the pigmentation of the zebrafish in a dose-dependent manner at low concentrations (fucoidan, <60 µg/mL; phloroglucinol, <10 µg/mL; fucosterol, <3 µg/mL). On the other hand, embryos treated with fucoxanthin at 200 µg/mL and 300 µg/mL exhibited either phenotypes of curved trunk or bent tail. Further validation work using dual antiplatelet therapy (DAPT) and dorsomorphin as positive controls suggest that fucoxanthin might target the Notch and bone morphogenetic protein (BMP) pathways, respectively. Findings from this exploratory study henceforth have demonstrated the utility of zebrafish embryo to accelerate the discovery of potential compounds for targeted anticancer therapy. © The Author(s) 2019 SAGE Publications 2019 Article PeerReviewed Rusdi, Nur 'Afina and Kue, Chin Siang and Yu, Ke Xin and Lau, Beng Fye and Chung, Lip Yong and Kiew, Lik Voon (2019) Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay. Natural Product Communications, 14 (6). 1934578X1985790. ISSN 1934-578X, DOI https://doi.org/10.1177/1934578X19857909 <https://doi.org/10.1177/1934578X19857909>. https://doi.org/10.1177/1934578X19857909 doi:10.1177/1934578X19857909 |
| spellingShingle | QR Microbiology R Medicine Rusdi, Nur 'Afina Kue, Chin Siang Yu, Ke Xin Lau, Beng Fye Chung, Lip Yong Kiew, Lik Voon Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay |
| title | Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay |
| title_full | Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay |
| title_fullStr | Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay |
| title_full_unstemmed | Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay |
| title_short | Assessment of Potential Anticancer Activity of Brown Seaweed Compounds Using Zebrafish Phenotypic Assay |
| title_sort | assessment of potential anticancer activity of brown seaweed compounds using zebrafish phenotypic assay |
| topic | QR Microbiology R Medicine |
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