Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein
A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount...
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| Format: | Article |
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Wiley
2019
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| author | Roslan, Amira Adlin Tayyab, Saad |
| author_facet | Roslan, Amira Adlin Tayyab, Saad |
| author_sort | Roslan, Amira Adlin |
| collection | UM |
| description | A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Treatment of the fluorescence quenching data according to the Stern–Volmer equation yielded the values of the Stern–Volmer constant (K sv ) and bimolecular quenching rate constant (k q ), whereas values of the binding constant (K a ) and number of binding sites (n) were obtained from the double logarithmic plot. This experiment provides an exciting opportunity for undergraduate students to independently perform ligand binding studies with a protein, in addition to providing the means for the determination of their binding parameters. © 2019 International Union of Biochemistry and Molecular Biology, 47(2): 156–160, 2019. © 2019 International Union of Biochemistry and Molecular Biology |
| first_indexed | 2024-03-06T06:00:25Z |
| format | Article |
| id | um.eprints-23634 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:00:25Z |
| publishDate | 2019 |
| publisher | Wiley |
| record_format | dspace |
| spelling | um.eprints-236342020-02-03T02:07:05Z http://eprints.um.edu.my/23634/ Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein Roslan, Amira Adlin Tayyab, Saad Q Science (General) QD Chemistry QH Natural history QR Microbiology A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Treatment of the fluorescence quenching data according to the Stern–Volmer equation yielded the values of the Stern–Volmer constant (K sv ) and bimolecular quenching rate constant (k q ), whereas values of the binding constant (K a ) and number of binding sites (n) were obtained from the double logarithmic plot. This experiment provides an exciting opportunity for undergraduate students to independently perform ligand binding studies with a protein, in addition to providing the means for the determination of their binding parameters. © 2019 International Union of Biochemistry and Molecular Biology, 47(2): 156–160, 2019. © 2019 International Union of Biochemistry and Molecular Biology Wiley 2019 Article PeerReviewed Roslan, Amira Adlin and Tayyab, Saad (2019) Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein. Biochemistry and Molecular Biology Education, 47 (2). pp. 156-160. ISSN 1470-8175, DOI https://doi.org/10.1002/bmb.21207 <https://doi.org/10.1002/bmb.21207>. https://doi.org/10.1002/bmb.21207 doi:10.1002/bmb.21207 |
| spellingShingle | Q Science (General) QD Chemistry QH Natural history QR Microbiology Roslan, Amira Adlin Tayyab, Saad Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein |
| title | Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein |
| title_full | Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein |
| title_fullStr | Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein |
| title_full_unstemmed | Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein |
| title_short | Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein |
| title_sort | exploring ligand protein interaction a laboratory exercise on herbicide binding to plasma transport protein |
| topic | Q Science (General) QD Chemistry QH Natural history QR Microbiology |
| work_keys_str_mv | AT roslanamiraadlin exploringligandproteininteractionalaboratoryexerciseonherbicidebindingtoplasmatransportprotein AT tayyabsaad exploringligandproteininteractionalaboratoryexerciseonherbicidebindingtoplasmatransportprotein |