First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia
There is an alarming increase in the prevalence of extended-spectrum β-lactamases (ESBLs) present mainly in Enterobacteriaceae and other nonfermenting gram-negative bacteria, such as Alcaligenes faecalis, which is the only species in that genus that is clinically relevant. We investigated Alcaligene...
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Institute of Infectious Diseases
2019
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| author | Puah, Suat Moi Puthucheary, Savithri Devi Chua, Kek Heng |
| author_facet | Puah, Suat Moi Puthucheary, Savithri Devi Chua, Kek Heng |
| author_sort | Puah, Suat Moi |
| collection | UM |
| description | There is an alarming increase in the prevalence of extended-spectrum β-lactamases (ESBLs) present mainly in Enterobacteriaceae and other nonfermenting gram-negative bacteria, such as Alcaligenes faecalis, which is the only species in that genus that is clinically relevant. We investigated Alcaligenes species from 7 cases (6 inpatients and one outpatient) at our tertiary-care hospital. Four patients had urinary tract infections, and one each had systemic lupus erythematosus, pulmonary stenosis, and diabetic ulcer. All 7 isolates were identified as Alcaligenes spp. based on their 16S rRNA gene sequences, and antibiotic susceptibility was determined using a Vitek 2 system with AST-GN87 cards. All the strains were resistant to cefazolin; 6 were resistant to trimethoprim/sulfamethoxazole; 5 manifested resistance to ampicillin/sulbactam, cefepime, tobramycin, ciprofloxacin, and nitrofurantoin; whereas 5 had multidrug resistance profiles. All the strains (7/7) expressed ESBL activity; PCR screening and sequencing showed evidence of genes blaTEM-116 (7/7) and blaOXA-10 (4/7), and we believe that this is the first report on the presence of TEM-116 and OXA-10 in an Alcaligenes spp. A combination of the 2 genes was present in 4 strains. All 7 strains were found to harbor at least one ESBL gene probably contributing to the drug resistance. © 2019, National Institute of Health. All rights reserved. |
| first_indexed | 2024-03-06T06:00:44Z |
| format | Article |
| id | um.eprints-23738 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:00:44Z |
| publishDate | 2019 |
| publisher | Institute of Infectious Diseases |
| record_format | dspace |
| spelling | um.eprints-237382020-02-11T03:56:41Z http://eprints.um.edu.my/23738/ First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia Puah, Suat Moi Puthucheary, Savithri Devi Chua, Kek Heng R Medicine There is an alarming increase in the prevalence of extended-spectrum β-lactamases (ESBLs) present mainly in Enterobacteriaceae and other nonfermenting gram-negative bacteria, such as Alcaligenes faecalis, which is the only species in that genus that is clinically relevant. We investigated Alcaligenes species from 7 cases (6 inpatients and one outpatient) at our tertiary-care hospital. Four patients had urinary tract infections, and one each had systemic lupus erythematosus, pulmonary stenosis, and diabetic ulcer. All 7 isolates were identified as Alcaligenes spp. based on their 16S rRNA gene sequences, and antibiotic susceptibility was determined using a Vitek 2 system with AST-GN87 cards. All the strains were resistant to cefazolin; 6 were resistant to trimethoprim/sulfamethoxazole; 5 manifested resistance to ampicillin/sulbactam, cefepime, tobramycin, ciprofloxacin, and nitrofurantoin; whereas 5 had multidrug resistance profiles. All the strains (7/7) expressed ESBL activity; PCR screening and sequencing showed evidence of genes blaTEM-116 (7/7) and blaOXA-10 (4/7), and we believe that this is the first report on the presence of TEM-116 and OXA-10 in an Alcaligenes spp. A combination of the 2 genes was present in 4 strains. All 7 strains were found to harbor at least one ESBL gene probably contributing to the drug resistance. © 2019, National Institute of Health. All rights reserved. Institute of Infectious Diseases 2019 Article PeerReviewed Puah, Suat Moi and Puthucheary, Savithri Devi and Chua, Kek Heng (2019) First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia. Japanese Journal of Infectious Diseases, 72 (4). pp. 266-269. ISSN 1344-6304, DOI https://doi.org/10.7883/yoken.JJID.2018.031 <https://doi.org/10.7883/yoken.JJID.2018.031>. https://doi.org/10.7883/yoken.JJID.2018.031 doi:10.7883/yoken.JJID.2018.031 |
| spellingShingle | R Medicine Puah, Suat Moi Puthucheary, Savithri Devi Chua, Kek Heng First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia |
| title | First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia |
| title_full | First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia |
| title_fullStr | First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia |
| title_full_unstemmed | First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia |
| title_short | First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia |
| title_sort | first report of extended spectrum β lactamases tem 116 and oxa 10 in clinical isolates of alcaligenes species from kuala lumpur malaysia |
| topic | R Medicine |
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