In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes
The present study explored phytochemicals, porcine pancreatic α-amylase (PPA) and lipase (PPL) inhibitory activities and antioxidant potential of polar and nonpolar extracts of the leaves and flowers of Ocimum basilicum and the in-silico mode of interaction between these enzymes and the major chemic...
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MDPI
2019
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| author | Noor, Zoy I. Ahmed, Dildar Rehman, Hafiz Muzzammel Qamar, Muhammad Tariq Froeyen, Matheus Ahmad, Sarfraz Mirza, Muhammad Usman |
| author_facet | Noor, Zoy I. Ahmed, Dildar Rehman, Hafiz Muzzammel Qamar, Muhammad Tariq Froeyen, Matheus Ahmad, Sarfraz Mirza, Muhammad Usman |
| author_sort | Noor, Zoy I. |
| collection | UM |
| description | The present study explored phytochemicals, porcine pancreatic α-amylase (PPA) and lipase (PPL) inhibitory activities and antioxidant potential of polar and nonpolar extracts of the leaves and flowers of Ocimum basilicum and the in-silico mode of interaction between these enzymes and the major chemical constituents of the herb. The hexane extract (HE) and hydro-ethanolic extract (EE) obtained sequentially were used to estimate PPA and PPL inhibitory and antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC). Chemical constituents of the essential oils and HE were determined by GC-MS (Gas Chromatography-Mass Spectrometry). For PPA inhibition, IC50 (µg/mL) of the extracts were 0.27–0.37, which were close to 0.24 of acarbose, while for PPL inhibition, IC50 (µg/mL) of the extracts were 278.40–399.65, and that of Orlistat 145.72. The flowers EE was most potent antioxidant followed by leaves EE. The leaves EE had highest TPC and TFC followed of flowers EE. The essential oil of flowers had higher estragole (55%) than linalool (37%), while the essential oil of the leaves had higher linalool (42%) than estragole (38%). The HE of the flowers contained higher estragole (42%) than linalool (23%), while of the HE of the leaves too had higher estragole (65%) than linalool (18%). The in-silico molecular docking study showed linalool and estragole to have considerable PPA and PPL binding potential, which were further investigated through molecular dynamics simulations and binding free energy calculations. The PPA and PPL inhibitory activities of O. basilicum extracts and their notable antioxidant potential propose the herb as a multi-target complimentary medicine for diabetes, obesity and oxidative stress. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. |
| first_indexed | 2024-03-06T06:00:58Z |
| format | Article |
| id | um.eprints-23818 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:00:58Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | um.eprints-238182020-02-18T01:35:03Z http://eprints.um.edu.my/23818/ In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes Noor, Zoy I. Ahmed, Dildar Rehman, Hafiz Muzzammel Qamar, Muhammad Tariq Froeyen, Matheus Ahmad, Sarfraz Mirza, Muhammad Usman Q Science (General) QD Chemistry The present study explored phytochemicals, porcine pancreatic α-amylase (PPA) and lipase (PPL) inhibitory activities and antioxidant potential of polar and nonpolar extracts of the leaves and flowers of Ocimum basilicum and the in-silico mode of interaction between these enzymes and the major chemical constituents of the herb. The hexane extract (HE) and hydro-ethanolic extract (EE) obtained sequentially were used to estimate PPA and PPL inhibitory and antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC). Chemical constituents of the essential oils and HE were determined by GC-MS (Gas Chromatography-Mass Spectrometry). For PPA inhibition, IC50 (µg/mL) of the extracts were 0.27–0.37, which were close to 0.24 of acarbose, while for PPL inhibition, IC50 (µg/mL) of the extracts were 278.40–399.65, and that of Orlistat 145.72. The flowers EE was most potent antioxidant followed by leaves EE. The leaves EE had highest TPC and TFC followed of flowers EE. The essential oil of flowers had higher estragole (55%) than linalool (37%), while the essential oil of the leaves had higher linalool (42%) than estragole (38%). The HE of the flowers contained higher estragole (42%) than linalool (23%), while of the HE of the leaves too had higher estragole (65%) than linalool (18%). The in-silico molecular docking study showed linalool and estragole to have considerable PPA and PPL binding potential, which were further investigated through molecular dynamics simulations and binding free energy calculations. The PPA and PPL inhibitory activities of O. basilicum extracts and their notable antioxidant potential propose the herb as a multi-target complimentary medicine for diabetes, obesity and oxidative stress. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. MDPI 2019 Article PeerReviewed Noor, Zoy I. and Ahmed, Dildar and Rehman, Hafiz Muzzammel and Qamar, Muhammad Tariq and Froeyen, Matheus and Ahmad, Sarfraz and Mirza, Muhammad Usman (2019) In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes. Biology, 8 (4). p. 92. ISSN 2079-7737, DOI https://doi.org/10.3390/biology8040092 <https://doi.org/10.3390/biology8040092>. https://doi.org/10.3390/biology8040092 doi:10.3390/biology8040092 |
| spellingShingle | Q Science (General) QD Chemistry Noor, Zoy I. Ahmed, Dildar Rehman, Hafiz Muzzammel Qamar, Muhammad Tariq Froeyen, Matheus Ahmad, Sarfraz Mirza, Muhammad Usman In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes |
| title | In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes |
| title_full | In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes |
| title_fullStr | In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes |
| title_full_unstemmed | In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes |
| title_short | In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes |
| title_sort | in vitro antidiabetic anti obesity and antioxidant analysis of ocimum basilicum aerial biomass and in silico molecular docking simulations with alpha amylase and lipase enzymes |
| topic | Q Science (General) QD Chemistry |
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