Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells
Bacterial biomolecule-mediated nanoparticle (NP) synthesis constitutes a reliable, eco-friendly approach that ameliorates green-chemistry principles. In this study, stable silver nanoparticles were synthesized by exposing aqueous silver ions to an extracellular diffusible pigment produced by Pseudom...
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| Format: | Article |
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Royal Society of Chemistry
2019
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| author | Muthukrishnan, Lakshmipathy Chellappa, Muralidharan Nanda, Anima Thukkaram, Sudhakar Selvaraj, Gracyfathima Muthiah, Bavanilatha Sagadevan, Suresh Lett, J. Anita |
| author_facet | Muthukrishnan, Lakshmipathy Chellappa, Muralidharan Nanda, Anima Thukkaram, Sudhakar Selvaraj, Gracyfathima Muthiah, Bavanilatha Sagadevan, Suresh Lett, J. Anita |
| author_sort | Muthukrishnan, Lakshmipathy |
| collection | UM |
| description | Bacterial biomolecule-mediated nanoparticle (NP) synthesis constitutes a reliable, eco-friendly approach that ameliorates green-chemistry principles. In this study, stable silver nanoparticles were synthesized by exposing aqueous silver ions to an extracellular diffusible pigment produced by Pseudomonas aeruginosa (PA6) under optimized laboratory conditions. Spectroscopic and microscopic analyses showed the typical characteristics of silver with an average size of ∼28.30 nm and spherical shape. The particles were polydispersed and showed no definite agglomeration with a zeta potential of -32.3 mV, conferring stability. Antimicrobial studies were carried out using 5, 15, 25 and 50 μg mL-1 concentrations of pcAgNPs, which showed significant antibacterial activity toward clinically important pathogens at all concentrations compared to with the control sample. The bactericidal effect induced by pcAgNPs associated with cell damage was well demonstrated using electron microscopic studies. ROS production was measured using the DCFH-DA method and the oxidative stress was assessed by measuring the reduced glutathione (GSH) levels. Cytotoxicity studies on HEp-2 (Human Epidermoid Larynx Carcinoma) cells exposed to pcAgNPs showed dose-dependent cytotoxic effect with IC50 of 14.8 μg mL-1 compared to with IC50 of 7.38 μg mL-1 for the Vero cell control. Mechanistically, the pcAgNPs activated p53 that induced catalase, leading to apoptosis and DNA fragmentation via a p53 transcriptional pathway and electron transport arrest, which resulted in cell death. This synergistic efficacy of pigment-AgNPs demonstrated excellent antimicrobial and anti-proliferative activities, providing a potential lead for developing a broad-spectrum antibacterial agent and improving the therapeutic modalities targeting carcinoma cells at the gene level. © 2019 The Royal Society of Chemistry. |
| first_indexed | 2024-03-06T06:01:14Z |
| format | Article |
| id | um.eprints-23912 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:01:14Z |
| publishDate | 2019 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | um.eprints-239122020-02-28T03:26:54Z http://eprints.um.edu.my/23912/ Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells Muthukrishnan, Lakshmipathy Chellappa, Muralidharan Nanda, Anima Thukkaram, Sudhakar Selvaraj, Gracyfathima Muthiah, Bavanilatha Sagadevan, Suresh Lett, J. Anita QC Physics QD Chemistry R Medicine TP Chemical technology Bacterial biomolecule-mediated nanoparticle (NP) synthesis constitutes a reliable, eco-friendly approach that ameliorates green-chemistry principles. In this study, stable silver nanoparticles were synthesized by exposing aqueous silver ions to an extracellular diffusible pigment produced by Pseudomonas aeruginosa (PA6) under optimized laboratory conditions. Spectroscopic and microscopic analyses showed the typical characteristics of silver with an average size of ∼28.30 nm and spherical shape. The particles were polydispersed and showed no definite agglomeration with a zeta potential of -32.3 mV, conferring stability. Antimicrobial studies were carried out using 5, 15, 25 and 50 μg mL-1 concentrations of pcAgNPs, which showed significant antibacterial activity toward clinically important pathogens at all concentrations compared to with the control sample. The bactericidal effect induced by pcAgNPs associated with cell damage was well demonstrated using electron microscopic studies. ROS production was measured using the DCFH-DA method and the oxidative stress was assessed by measuring the reduced glutathione (GSH) levels. Cytotoxicity studies on HEp-2 (Human Epidermoid Larynx Carcinoma) cells exposed to pcAgNPs showed dose-dependent cytotoxic effect with IC50 of 14.8 μg mL-1 compared to with IC50 of 7.38 μg mL-1 for the Vero cell control. Mechanistically, the pcAgNPs activated p53 that induced catalase, leading to apoptosis and DNA fragmentation via a p53 transcriptional pathway and electron transport arrest, which resulted in cell death. This synergistic efficacy of pigment-AgNPs demonstrated excellent antimicrobial and anti-proliferative activities, providing a potential lead for developing a broad-spectrum antibacterial agent and improving the therapeutic modalities targeting carcinoma cells at the gene level. © 2019 The Royal Society of Chemistry. Royal Society of Chemistry 2019 Article PeerReviewed Muthukrishnan, Lakshmipathy and Chellappa, Muralidharan and Nanda, Anima and Thukkaram, Sudhakar and Selvaraj, Gracyfathima and Muthiah, Bavanilatha and Sagadevan, Suresh and Lett, J. Anita (2019) Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells. RSC Advances, 9 (28). pp. 15874-15886. ISSN 2046-2069, DOI https://doi.org/10.1039/c9ra01072f <https://doi.org/10.1039/c9ra01072f>. https://doi.org/10.1039/c9ra01072f doi:10.1039/c9ra01072f |
| spellingShingle | QC Physics QD Chemistry R Medicine TP Chemical technology Muthukrishnan, Lakshmipathy Chellappa, Muralidharan Nanda, Anima Thukkaram, Sudhakar Selvaraj, Gracyfathima Muthiah, Bavanilatha Sagadevan, Suresh Lett, J. Anita Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells |
| title | Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells |
| title_full | Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells |
| title_fullStr | Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells |
| title_full_unstemmed | Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells |
| title_short | Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells |
| title_sort | bio fabrication of pigment capped silver nanoparticles encountering antibiotic resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma hep 2 cells |
| topic | QC Physics QD Chemistry R Medicine TP Chemical technology |
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