The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia
Objective: Colistin is the last line of therapy for infections caused by multidrug-resistant Gram-negative bacteria. The objective of this study was to determine the phenotypic and genotypic characteristics of colistin-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from swine samples in Ma...
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Elsevier
2019
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| _version_ | 1796961937477599232 |
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| author | Mobasseri, Golnaz Teh, Cindy Shuan Ju Ooi, Peck Toung Thong, Kwai Lin |
| author_facet | Mobasseri, Golnaz Teh, Cindy Shuan Ju Ooi, Peck Toung Thong, Kwai Lin |
| author_sort | Mobasseri, Golnaz |
| collection | UM |
| description | Objective: Colistin is the last line of therapy for infections caused by multidrug-resistant Gram-negative bacteria. The objective of this study was to determine the phenotypic and genotypic characteristics of colistin-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from swine samples in Malaysia. Methods: A total of 46 swine K. pneumoniae strains isolated from 2013–2015 in Malaysia were analysed for the production of extended-spectrum β-lactamases and carbapenemase. The resistance traits and genetic diversity of these strains were characterised by polymerase chain reaction, conjugation, plasmid analysis, and pulsed-field gel electrophoresis. Results: Nineteen of 46 strains were multidrug resistant while 13 were resistant to colistin. The majority of colistin-resistant strains harboured blaTEM gene (92.3%), followed by blaSHV (69.23%), blaCTXM-1 (38.46%), and blaMCR-1 (23.08%). All three colistin-resistant strains had transferable plasmids and the colistin resistance gene blaMCR-1. Genotyping by pulsed-field gel electrophoresis showed high genetic diversity among the K. pneumoniae and that the colistin-resistant K. pneumoniae strains were heterogenous. Conclusion: It is believed that this is the first report of colistin-resistant K. pneumoniae among swine strains associated with mcr-1 plasmid in Malaysia. Due to the emergence of β-lactam, carbapenem and colistin resistance, the use of colistin in animal husbandry and agriculture should be avoided to prevent treatment failure. © 2019 International Society for Chemotherapy of Infection and Cancer |
| first_indexed | 2024-03-06T06:01:34Z |
| format | Article |
| id | um.eprints-24021 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:01:34Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-240212020-03-12T02:20:34Z http://eprints.um.edu.my/24021/ The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia Mobasseri, Golnaz Teh, Cindy Shuan Ju Ooi, Peck Toung Thong, Kwai Lin Q Science (General) QH Natural history R Medicine Objective: Colistin is the last line of therapy for infections caused by multidrug-resistant Gram-negative bacteria. The objective of this study was to determine the phenotypic and genotypic characteristics of colistin-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from swine samples in Malaysia. Methods: A total of 46 swine K. pneumoniae strains isolated from 2013–2015 in Malaysia were analysed for the production of extended-spectrum β-lactamases and carbapenemase. The resistance traits and genetic diversity of these strains were characterised by polymerase chain reaction, conjugation, plasmid analysis, and pulsed-field gel electrophoresis. Results: Nineteen of 46 strains were multidrug resistant while 13 were resistant to colistin. The majority of colistin-resistant strains harboured blaTEM gene (92.3%), followed by blaSHV (69.23%), blaCTXM-1 (38.46%), and blaMCR-1 (23.08%). All three colistin-resistant strains had transferable plasmids and the colistin resistance gene blaMCR-1. Genotyping by pulsed-field gel electrophoresis showed high genetic diversity among the K. pneumoniae and that the colistin-resistant K. pneumoniae strains were heterogenous. Conclusion: It is believed that this is the first report of colistin-resistant K. pneumoniae among swine strains associated with mcr-1 plasmid in Malaysia. Due to the emergence of β-lactam, carbapenem and colistin resistance, the use of colistin in animal husbandry and agriculture should be avoided to prevent treatment failure. © 2019 International Society for Chemotherapy of Infection and Cancer Elsevier 2019 Article PeerReviewed Mobasseri, Golnaz and Teh, Cindy Shuan Ju and Ooi, Peck Toung and Thong, Kwai Lin (2019) The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia. Journal of Global Antimicrobial Resistance, 17. pp. 227-232. ISSN 2213-7165, DOI https://doi.org/10.1016/j.jgar.2018.12.015 <https://doi.org/10.1016/j.jgar.2018.12.015>. https://doi.org/10.1016/j.jgar.2018.12.015 doi:10.1016/j.jgar.2018.12.015 |
| spellingShingle | Q Science (General) QH Natural history R Medicine Mobasseri, Golnaz Teh, Cindy Shuan Ju Ooi, Peck Toung Thong, Kwai Lin The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia |
| title | The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia |
| title_full | The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia |
| title_fullStr | The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia |
| title_full_unstemmed | The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia |
| title_short | The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia |
| title_sort | emergence of colistin resistant klebsiella pneumoniae strains from swine in malaysia |
| topic | Q Science (General) QH Natural history R Medicine |
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