Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress
Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) fro...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Canadian Science Publishing (NRC Research Press)
2020
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| Online Access: | http://eprints.um.edu.my/24022/1/artilceall.pdf |
| _version_ | 1825722170934820864 |
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| author | Sultana, Shahnaz Paul, Sumonta C. Parveen, Samia Alam, Saiful Rahman, Naziza Jannat, Bushra Hoque, Sirajul Rahman, Mohammad Tariqur Karim, Muhammad Manjurul |
| author_facet | Sultana, Shahnaz Paul, Sumonta C. Parveen, Samia Alam, Saiful Rahman, Naziza Jannat, Bushra Hoque, Sirajul Rahman, Mohammad Tariqur Karim, Muhammad Manjurul |
| author_sort | Sultana, Shahnaz |
| collection | UM |
| description | Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) from saline and nonsaline areas in Bangladesh where electrical conductivity was measured as >7.45 and <1.80 dS/m, respectively. Bacteria isolated from saline areas were able to grow in a salt concentration of up to 2.60 mol/L, contrary to the isolates collected from non-saline areas that did not survive beyond 854 mmol/L. Among the salt-tolerant isolates, Bacillus aryabhattai, Achromobacter denitrificans, and Ochrobactrum intermedium, identified by comparing respective sequences of 16S rRNA using the NCBI GenBank, exhibited a higher amount of atmospheric nitrogen fixation, phosphate solubilization, and indoleacetic acid production at 200 mmol/L salt stress. Salt-tolerant isolates exhibited greater resistance to heavy metals and antibiotics, which could be due to the production of an exopolysaccharide layer outside the cell surface. Oryza sativa L. fertilized with B. aryabhattai MS3 and grown under 200 mmol/L salt stress was found to be favoured by enhanced expression of a set of at least four salt-responsive plant genes: BZ8, SOS1, GIG, and NHX1. Fertilization of rice with osmoprotectant-producing PGPR, therefore, could be a climate-change-preparedness strategy for coastal agriculture. |
| first_indexed | 2024-03-06T06:01:34Z |
| format | Article |
| id | um.eprints-24022 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T06:01:34Z |
| publishDate | 2020 |
| publisher | Canadian Science Publishing (NRC Research Press) |
| record_format | dspace |
| spelling | um.eprints-240222020-03-12T03:22:01Z http://eprints.um.edu.my/24022/ Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress Sultana, Shahnaz Paul, Sumonta C. Parveen, Samia Alam, Saiful Rahman, Naziza Jannat, Bushra Hoque, Sirajul Rahman, Mohammad Tariqur Karim, Muhammad Manjurul QH301 Biology S Agriculture (General) Growth and productivity of rice are negatively affected by soil salinity. However, some salt-tolerant rhizosphere-inhabiting bacteria can improve salt resistance of plants, thereby augmenting plant growth and production. Here, we isolated a total of 53 plant-growth-promoting rhizobacteria (PGPR) from saline and nonsaline areas in Bangladesh where electrical conductivity was measured as >7.45 and <1.80 dS/m, respectively. Bacteria isolated from saline areas were able to grow in a salt concentration of up to 2.60 mol/L, contrary to the isolates collected from non-saline areas that did not survive beyond 854 mmol/L. Among the salt-tolerant isolates, Bacillus aryabhattai, Achromobacter denitrificans, and Ochrobactrum intermedium, identified by comparing respective sequences of 16S rRNA using the NCBI GenBank, exhibited a higher amount of atmospheric nitrogen fixation, phosphate solubilization, and indoleacetic acid production at 200 mmol/L salt stress. Salt-tolerant isolates exhibited greater resistance to heavy metals and antibiotics, which could be due to the production of an exopolysaccharide layer outside the cell surface. Oryza sativa L. fertilized with B. aryabhattai MS3 and grown under 200 mmol/L salt stress was found to be favoured by enhanced expression of a set of at least four salt-responsive plant genes: BZ8, SOS1, GIG, and NHX1. Fertilization of rice with osmoprotectant-producing PGPR, therefore, could be a climate-change-preparedness strategy for coastal agriculture. Canadian Science Publishing (NRC Research Press) 2020 Article PeerReviewed text en http://eprints.um.edu.my/24022/1/artilceall.pdf Sultana, Shahnaz and Paul, Sumonta C. and Parveen, Samia and Alam, Saiful and Rahman, Naziza and Jannat, Bushra and Hoque, Sirajul and Rahman, Mohammad Tariqur and Karim, Muhammad Manjurul (2020) Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress. Canadian Journal of Microbiology, 66 (2). pp. 144-160. ISSN 0008-4166, DOI https://doi.org/10.1139/cjm-2019-0323 <https://doi.org/10.1139/cjm-2019-0323>. https://www.nrcresearchpress.com/doi/10.1139/cjm-2019-0323 doi:10.1139/cjm-2019-0323 |
| spellingShingle | QH301 Biology S Agriculture (General) Sultana, Shahnaz Paul, Sumonta C. Parveen, Samia Alam, Saiful Rahman, Naziza Jannat, Bushra Hoque, Sirajul Rahman, Mohammad Tariqur Karim, Muhammad Manjurul Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress |
| title | Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress |
| title_full | Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress |
| title_fullStr | Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress |
| title_full_unstemmed | Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress |
| title_short | Isolation and identification of salt-tolerant plant-growth-promoting rhizobacteria and their application for rice cultivation under salt stress |
| title_sort | isolation and identification of salt tolerant plant growth promoting rhizobacteria and their application for rice cultivation under salt stress |
| topic | QH301 Biology S Agriculture (General) |
| url | http://eprints.um.edu.my/24022/1/artilceall.pdf |
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