Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats
The present work examined ex vivo the acute effect of quercetin on diabetic rat aortic ring reactivity in response to endotheliumdependent (acetylcholine, ACh) and endothelium-independent (sodium nitroprusside, SNP) relaxants, and to the a1-adrenergic agonist phenylephrine (PE). Responses were compa...
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| Format: | Article |
| Language: | English |
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Elsevier
2005
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| Online Access: | http://eprints.um.edu.my/2410/1/Effect_of_quercetin_on_altered_vascular_reactivity_in_aortas_isolated_from_streptozotocin-induced_diabetic_rats.pdf |
| _version_ | 1796944858012712960 |
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| author | Ajay, Machha Achike, Francis I. Mustafa, Ali Mohd Mustafa, Mohd Rais |
| author_facet | Ajay, Machha Achike, Francis I. Mustafa, Ali Mohd Mustafa, Mohd Rais |
| author_sort | Ajay, Machha |
| collection | UM |
| description | The present work examined ex vivo the acute effect of quercetin on diabetic rat aortic ring reactivity in response to endotheliumdependent (acetylcholine, ACh) and endothelium-independent (sodium nitroprusside, SNP) relaxants, and to the a1-adrenergic agonist phenylephrine (PE). Responses were compared to those of aortic rings from age- and sex-matched euglycemic rats. Compared to euglycemic rat aortic rings, diabetic rings showed less relaxation in response to ACh and SNP, and greater contraction
in response to PE. Pretreatment with quercetin (10 mM, 20 min) increased ACh-induced relaxation and decreased PE-induced contraction in diabetic, but did not affect euglycemic rat aortic ring responses. Following pretreatment with the nitric oxide synthase inhibitor Nv-nitro-L-arginine methyl ester (L-NAME, 10 mM), quercetin reduced PE-induced contractions in both aortic ring types,
although L-NAME attenuated the reduction in the diabetic rings. Quercetin did not alter SNP vasodilatory effects in either ring type compared to their respective controls. These findings indicate that quercetin acutely improved vascular responsiveness in blood vessels from diabetic rats, and that these effects were mediated, at least in part, by enhanced endothelial nitric oxide bioavailability.
These effects of quercetin suggest the possible beneficial effects of quercetin in vivo in experimental diabetes and possibly in other cardiovascular diseases. |
| first_indexed | 2024-03-06T05:07:59Z |
| format | Article |
| id | um.eprints-2410 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:07:59Z |
| publishDate | 2005 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-24102019-12-18T06:20:26Z http://eprints.um.edu.my/2410/ Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats Ajay, Machha Achike, Francis I. Mustafa, Ali Mohd Mustafa, Mohd Rais RM Therapeutics. Pharmacology SF Animal culture The present work examined ex vivo the acute effect of quercetin on diabetic rat aortic ring reactivity in response to endotheliumdependent (acetylcholine, ACh) and endothelium-independent (sodium nitroprusside, SNP) relaxants, and to the a1-adrenergic agonist phenylephrine (PE). Responses were compared to those of aortic rings from age- and sex-matched euglycemic rats. Compared to euglycemic rat aortic rings, diabetic rings showed less relaxation in response to ACh and SNP, and greater contraction in response to PE. Pretreatment with quercetin (10 mM, 20 min) increased ACh-induced relaxation and decreased PE-induced contraction in diabetic, but did not affect euglycemic rat aortic ring responses. Following pretreatment with the nitric oxide synthase inhibitor Nv-nitro-L-arginine methyl ester (L-NAME, 10 mM), quercetin reduced PE-induced contractions in both aortic ring types, although L-NAME attenuated the reduction in the diabetic rings. Quercetin did not alter SNP vasodilatory effects in either ring type compared to their respective controls. These findings indicate that quercetin acutely improved vascular responsiveness in blood vessels from diabetic rats, and that these effects were mediated, at least in part, by enhanced endothelial nitric oxide bioavailability. These effects of quercetin suggest the possible beneficial effects of quercetin in vivo in experimental diabetes and possibly in other cardiovascular diseases. Elsevier 2005-12-27 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2410/1/Effect_of_quercetin_on_altered_vascular_reactivity_in_aortas_isolated_from_streptozotocin-induced_diabetic_rats.pdf Ajay, Machha and Achike, Francis I. and Mustafa, Ali Mohd and Mustafa, Mohd Rais (2005) Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats. Diabetes Research and Clinical Practice, 73 (1). pp. 1-7. ISSN 0168-8227, |
| spellingShingle | RM Therapeutics. Pharmacology SF Animal culture Ajay, Machha Achike, Francis I. Mustafa, Ali Mohd Mustafa, Mohd Rais Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats |
| title | Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats |
| title_full | Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats |
| title_fullStr | Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats |
| title_full_unstemmed | Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats |
| title_short | Effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats |
| title_sort | effect of quercetin on altered vascular reactivity in aortas isolated from streptozotocin induced diabetic rats |
| topic | RM Therapeutics. Pharmacology SF Animal culture |
| url | http://eprints.um.edu.my/2410/1/Effect_of_quercetin_on_altered_vascular_reactivity_in_aortas_isolated_from_streptozotocin-induced_diabetic_rats.pdf |
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