The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats
Boldine, a major aporphine alkaloid found in Chilean boldo tree, is a potent antioxidant. Oxidative stress plays a detrimentalrole in the pathogenesis of endothelial dysfunction in hypertension. In the present study, we investigated the effects of boldine on endothelial dysfunction in hypertension u...
| Hoofdauteurs: | , , , , |
|---|---|
| Formaat: | Artikel |
| Taal: | English |
| Gepubliceerd in: |
2011
|
| Onderwerpen: | |
| Online toegang: | http://eprints.um.edu.my/2413/1/Experimental_Biology_and_Medicine_2011__1%E2%80%93_6.pdf |
| _version_ | 1825718673860460544 |
|---|---|
| author | Lau, Yeh-Siang Machha, A. Achike, F.I. Mustafa, Mohd Rais Murugan, D. |
| author_facet | Lau, Yeh-Siang Machha, A. Achike, F.I. Mustafa, Mohd Rais Murugan, D. |
| author_sort | Lau, Yeh-Siang |
| collection | UM |
| description | Boldine, a major aporphine alkaloid found in Chilean boldo tree, is a potent antioxidant. Oxidative stress plays a detrimentalrole in the pathogenesis of endothelial dysfunction in hypertension. In the present study, we investigated the effects of boldine on endothelial dysfunction in hypertension using spontaneously hypertensive rats (SHR), the most studied animal model of hypertension. SHR and their age-matched normotensive Wistar-Kyoto (WKY) rats were treated with boldine (20 mg/kg perday) or its vehicle, which served as control, for seven days. Control SHR displayed higher systolic blood pressure (SBP), reduced endothelium-dependent aortic relaxation to acetylcholine (ACh), marginally attenuated endothelium-independent aortic relaxation to sodium nitroprusside (SNP), increased aortic superoxide and peroxynitrite production, and enhanced p47 phox protein expression as compared with control WKY rats. Boldine treatment significantly lowered SBP in SHR but not in WKY. Boldine treatment enhanced the maximal relaxation to ACh in SHR, but had no effect in WKY, whereas the sensitivity to ACh was increased in both SHR and WKY aortas. Boldine treatment enhanced sensitivity, but was without effect on maximal aortic relaxation responses, to SNP in both WKY and SHR aortas. In addition, boldine treatment lowered aortic superoxide and peroxynitrite production and downregulated p47 phox protein expression in SHR aortas, but had no effect in the WKY control. These results show that boldine treatment exerts endothelial protective effects in hypertension, achieved, at least in part, through the inhibition of NADPH-mediated superoxide production. |
| first_indexed | 2024-03-06T05:08:00Z |
| format | Article |
| id | um.eprints-2413 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:08:00Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | um.eprints-24132019-12-18T06:38:02Z http://eprints.um.edu.my/2413/ The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats Lau, Yeh-Siang Machha, A. Achike, F.I. Mustafa, Mohd Rais Murugan, D. R Medicine RM Therapeutics. Pharmacology Boldine, a major aporphine alkaloid found in Chilean boldo tree, is a potent antioxidant. Oxidative stress plays a detrimentalrole in the pathogenesis of endothelial dysfunction in hypertension. In the present study, we investigated the effects of boldine on endothelial dysfunction in hypertension using spontaneously hypertensive rats (SHR), the most studied animal model of hypertension. SHR and their age-matched normotensive Wistar-Kyoto (WKY) rats were treated with boldine (20 mg/kg perday) or its vehicle, which served as control, for seven days. Control SHR displayed higher systolic blood pressure (SBP), reduced endothelium-dependent aortic relaxation to acetylcholine (ACh), marginally attenuated endothelium-independent aortic relaxation to sodium nitroprusside (SNP), increased aortic superoxide and peroxynitrite production, and enhanced p47 phox protein expression as compared with control WKY rats. Boldine treatment significantly lowered SBP in SHR but not in WKY. Boldine treatment enhanced the maximal relaxation to ACh in SHR, but had no effect in WKY, whereas the sensitivity to ACh was increased in both SHR and WKY aortas. Boldine treatment enhanced sensitivity, but was without effect on maximal aortic relaxation responses, to SNP in both WKY and SHR aortas. In addition, boldine treatment lowered aortic superoxide and peroxynitrite production and downregulated p47 phox protein expression in SHR aortas, but had no effect in the WKY control. These results show that boldine treatment exerts endothelial protective effects in hypertension, achieved, at least in part, through the inhibition of NADPH-mediated superoxide production. 2011-12-07 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2413/1/Experimental_Biology_and_Medicine_2011__1%E2%80%93_6.pdf Lau, Yeh-Siang and Machha, A. and Achike, F.I. and Mustafa, Mohd Rais and Murugan, D. (2011) The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats. Experimental Biology and Medicine. ISSN 1535-3702, http://ebm.rsmjournals.com/content/early/2011/12/06/ebm.2011.011145.long |
| spellingShingle | R Medicine RM Therapeutics. Pharmacology Lau, Yeh-Siang Machha, A. Achike, F.I. Mustafa, Mohd Rais Murugan, D. The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| title | The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| title_full | The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| title_fullStr | The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| title_full_unstemmed | The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| title_short | The aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| title_sort | aporphine alkaloid boldine improves endothelial function in spontaneously hypertensive rats |
| topic | R Medicine RM Therapeutics. Pharmacology |
| url | http://eprints.um.edu.my/2413/1/Experimental_Biology_and_Medicine_2011__1%E2%80%93_6.pdf |
| work_keys_str_mv | AT lauyehsiang theaporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT machhaa theaporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT achikefi theaporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT mustafamohdrais theaporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT murugand theaporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT lauyehsiang aporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT machhaa aporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT achikefi aporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT mustafamohdrais aporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats AT murugand aporphinealkaloidboldineimprovesendothelialfunctioninspontaneouslyhypertensiverats |