Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production

The waste seeds of Leucaena leucocephala (LLS) used in this study were unused residues obtained after oil and polysaccharides extraction. The microcrystalline cellulose (MCC) was isolated from LLS by acid treatment. MCC produced was, then, further converted to glucose by using sulphuric acid at 121...

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Main Authors: Husin, Maryam, Rahim, Nurnadiah, Ahmad, Mohd Radzi, Romli, Ahmad Zafir, Ilham, Zul
Format: Article
Language:English
Published: Penerbit UTM Press, Universiti Teknologi Malaysia 2019
Subjects:
Online Access:http://eprints.um.edu.my/24162/1/Husin_M_MJFAS_2019.pdf
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author Husin, Maryam
Rahim, Nurnadiah
Ahmad, Mohd Radzi
Romli, Ahmad Zafir
Ilham, Zul
author_facet Husin, Maryam
Rahim, Nurnadiah
Ahmad, Mohd Radzi
Romli, Ahmad Zafir
Ilham, Zul
author_sort Husin, Maryam
collection UM
description The waste seeds of Leucaena leucocephala (LLS) used in this study were unused residues obtained after oil and polysaccharides extraction. The microcrystalline cellulose (MCC) was isolated from LLS by acid treatment. MCC produced was, then, further converted to glucose by using sulphuric acid at 121 °C by varying the acid concentration and reaction time. The sugar composition was analyzed by using the phenol-sulfuric acid method and pre-column derivatization HPLC technique. The yield of glucose ranging from 70–85% could be obtained from MCC hydrolyzates, depending on the hydrolysis factors, which corresponding to around 57-75% of the percentage conversion of MCC to glucose.Cellulose isolated from LLS was, therefore, potentially suitable to be utilized in liquid biofuels and other value-added chemicals such as bioethanol, 5-hydroxymethylfurfural(HMF), and levulinic acid.
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spelling um.eprints-241622020-04-09T04:33:10Z http://eprints.um.edu.my/24162/ Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production Husin, Maryam Rahim, Nurnadiah Ahmad, Mohd Radzi Romli, Ahmad Zafir Ilham, Zul Q Science (General) QK Botany T Technology (General) TD Environmental technology. Sanitary engineering TP Chemical technology The waste seeds of Leucaena leucocephala (LLS) used in this study were unused residues obtained after oil and polysaccharides extraction. The microcrystalline cellulose (MCC) was isolated from LLS by acid treatment. MCC produced was, then, further converted to glucose by using sulphuric acid at 121 °C by varying the acid concentration and reaction time. The sugar composition was analyzed by using the phenol-sulfuric acid method and pre-column derivatization HPLC technique. The yield of glucose ranging from 70–85% could be obtained from MCC hydrolyzates, depending on the hydrolysis factors, which corresponding to around 57-75% of the percentage conversion of MCC to glucose.Cellulose isolated from LLS was, therefore, potentially suitable to be utilized in liquid biofuels and other value-added chemicals such as bioethanol, 5-hydroxymethylfurfural(HMF), and levulinic acid. Penerbit UTM Press, Universiti Teknologi Malaysia 2019 Article PeerReviewed text en http://eprints.um.edu.my/24162/1/Husin_M_MJFAS_2019.pdf Husin, Maryam and Rahim, Nurnadiah and Ahmad, Mohd Radzi and Romli, Ahmad Zafir and Ilham, Zul (2019) Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production. Malaysian Journal of Fundamental and Applied Sciences, 15 (2). pp. 200-205. ISSN 2289-5981, DOI https://doi.org/10.11113/mjfas.v15n2.1165 <https://doi.org/10.11113/mjfas.v15n2.1165>. https://doi.org/10.11113/mjfas.v15n2.1165 doi:10.11113/mjfas.v15n2.1165
spellingShingle Q Science (General)
QK Botany
T Technology (General)
TD Environmental technology. Sanitary engineering
TP Chemical technology
Husin, Maryam
Rahim, Nurnadiah
Ahmad, Mohd Radzi
Romli, Ahmad Zafir
Ilham, Zul
Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production
title Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production
title_full Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production
title_fullStr Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production
title_full_unstemmed Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production
title_short Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production
title_sort hydrolysis of microcrystalline cellulose isolated from waste seeds of leucaena leucocephala for glucose production
topic Q Science (General)
QK Botany
T Technology (General)
TD Environmental technology. Sanitary engineering
TP Chemical technology
url http://eprints.um.edu.my/24162/1/Husin_M_MJFAS_2019.pdf
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