A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth
Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to...
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Medknow Publications
2019
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| author | Ahmad, Islamudin Ambarwati, Neneng Siti Silfi Elya, Berna Omar, Hanita Mulia, Kamarza Yanuar, Arry Negishi, Osamu Mun'im, Abdul |
| author_facet | Ahmad, Islamudin Ambarwati, Neneng Siti Silfi Elya, Berna Omar, Hanita Mulia, Kamarza Yanuar, Arry Negishi, Osamu Mun'im, Abdul |
| author_sort | Ahmad, Islamudin |
| collection | UM |
| description | Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug. © 2019 Wolters Kluwer Medknow Publications. All rights reserved. |
| first_indexed | 2024-03-06T06:02:32Z |
| format | Article |
| id | um.eprints-24349 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:02:32Z |
| publishDate | 2019 |
| publisher | Medknow Publications |
| record_format | dspace |
| spelling | um.eprints-243492020-05-19T03:26:37Z http://eprints.um.edu.my/24349/ A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth Ahmad, Islamudin Ambarwati, Neneng Siti Silfi Elya, Berna Omar, Hanita Mulia, Kamarza Yanuar, Arry Negishi, Osamu Mun'im, Abdul QD Chemistry RS Pharmacy and materia medica Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug. © 2019 Wolters Kluwer Medknow Publications. All rights reserved. Medknow Publications 2019 Article PeerReviewed Ahmad, Islamudin and Ambarwati, Neneng Siti Silfi and Elya, Berna and Omar, Hanita and Mulia, Kamarza and Yanuar, Arry and Negishi, Osamu and Mun'im, Abdul (2019) A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth. Asian Pacific Journal of Tropical Biomedicine, 9 (6). pp. 257-262. ISSN 2221-1691, DOI https://doi.org/10.4103/2221-1691.260398 <https://doi.org/10.4103/2221-1691.260398>. https://doi.org/10.4103/2221-1691.260398 doi:10.4103/2221-1691.260398 |
| spellingShingle | QD Chemistry RS Pharmacy and materia medica Ahmad, Islamudin Ambarwati, Neneng Siti Silfi Elya, Berna Omar, Hanita Mulia, Kamarza Yanuar, Arry Negishi, Osamu Mun'im, Abdul A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
| title | A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
| title_full | A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
| title_fullStr | A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
| title_full_unstemmed | A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
| title_short | A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
| title_sort | new angiotensin converting enzyme inhibitor from peperomia pellucida l kunth |
| topic | QD Chemistry RS Pharmacy and materia medica |
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