Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology

This work is a study of the performance and effect of operational parameters on biohydrogen production from palm oil mill effluent by dark fermentation in batch mode. The tests were conducted with samples prepared in 150 mL bottles using a shaker at 150 rpm. Response surface methodology was applied...

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Main Authors: Akhbari, Azam, Zinatizadeh, Ali Akbar, Vafaeifard, Mohsen, Mohammadi, Parviz, Zainal, Bidattul Syirat, Ibrahim, Shaliza
Format: Article
Published: Desalination Publications 2019
Subjects:
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author Akhbari, Azam
Zinatizadeh, Ali Akbar
Vafaeifard, Mohsen
Mohammadi, Parviz
Zainal, Bidattul Syirat
Ibrahim, Shaliza
author_facet Akhbari, Azam
Zinatizadeh, Ali Akbar
Vafaeifard, Mohsen
Mohammadi, Parviz
Zainal, Bidattul Syirat
Ibrahim, Shaliza
author_sort Akhbari, Azam
collection UM
description This work is a study of the performance and effect of operational parameters on biohydrogen production from palm oil mill effluent by dark fermentation in batch mode. The tests were conducted with samples prepared in 150 mL bottles using a shaker at 150 rpm. Response surface methodology was applied to investigate the influence of the four significant independent parameters viz. pH (5, 5.5, and 6), temperature (30°C, 35°C, and 40°C), substrate concentration (5,000, 12,500, and 20,000 mg L –1 ) and inoculum–substrate ratios of 2, 0.8, and 0.5 (expressed as volatile suspended solid (VSS) basis) with the inoculum concentration of 10 g L –1 VSS on biohydrogen production. All the experiments were analyzed at the incubation time of 8, 16, and 24 h. Upon seeing each interval, the results were compared. The highest chemical oxygen demand (COD) removal, the hydrogen content in the biogas as hydrogen percentage (H 2 %), and hydrogen yield (HY) were obtained 58.3%, 80%, and 3.63 mol H 2 mol –1 glucose, respectively, at 24 h incubation time. An overlay study was done to find an overall optimization of the parameters. The optimized conditions were COD removal 49%, HY 3.2 mol H 2 mol –1 glucose, and hydrogen percentage 80%. Also, the Monod model was studied to calculate the kinetics constants of the maximum substrate utilization rate (U max ) and half-velocity K s which are found to be 0.261 g L –1 d –1 and 0.349 mg L –1 , respectively. © 2019 Desalination Publications. All rights reserved.
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spelling um.eprints-243792020-05-27T05:00:06Z http://eprints.um.edu.my/24379/ Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology Akhbari, Azam Zinatizadeh, Ali Akbar Vafaeifard, Mohsen Mohammadi, Parviz Zainal, Bidattul Syirat Ibrahim, Shaliza QD Chemistry TA Engineering (General). Civil engineering (General) This work is a study of the performance and effect of operational parameters on biohydrogen production from palm oil mill effluent by dark fermentation in batch mode. The tests were conducted with samples prepared in 150 mL bottles using a shaker at 150 rpm. Response surface methodology was applied to investigate the influence of the four significant independent parameters viz. pH (5, 5.5, and 6), temperature (30°C, 35°C, and 40°C), substrate concentration (5,000, 12,500, and 20,000 mg L –1 ) and inoculum–substrate ratios of 2, 0.8, and 0.5 (expressed as volatile suspended solid (VSS) basis) with the inoculum concentration of 10 g L –1 VSS on biohydrogen production. All the experiments were analyzed at the incubation time of 8, 16, and 24 h. Upon seeing each interval, the results were compared. The highest chemical oxygen demand (COD) removal, the hydrogen content in the biogas as hydrogen percentage (H 2 %), and hydrogen yield (HY) were obtained 58.3%, 80%, and 3.63 mol H 2 mol –1 glucose, respectively, at 24 h incubation time. An overlay study was done to find an overall optimization of the parameters. The optimized conditions were COD removal 49%, HY 3.2 mol H 2 mol –1 glucose, and hydrogen percentage 80%. Also, the Monod model was studied to calculate the kinetics constants of the maximum substrate utilization rate (U max ) and half-velocity K s which are found to be 0.261 g L –1 d –1 and 0.349 mg L –1 , respectively. © 2019 Desalination Publications. All rights reserved. Desalination Publications 2019 Article PeerReviewed Akhbari, Azam and Zinatizadeh, Ali Akbar and Vafaeifard, Mohsen and Mohammadi, Parviz and Zainal, Bidattul Syirat and Ibrahim, Shaliza (2019) Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology. Desalination and Water Treatment, 137. pp. 101-113. ISSN 1944-3994, DOI https://doi.org/10.5004/dwt.2019.23169 <https://doi.org/10.5004/dwt.2019.23169>. https://doi.org/10.5004/dwt.2019.23169 doi:10.5004/dwt.2019.23169
spellingShingle QD Chemistry
TA Engineering (General). Civil engineering (General)
Akhbari, Azam
Zinatizadeh, Ali Akbar
Vafaeifard, Mohsen
Mohammadi, Parviz
Zainal, Bidattul Syirat
Ibrahim, Shaliza
Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
title Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
title_full Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
title_fullStr Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
title_full_unstemmed Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
title_short Effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
title_sort effect of operational variables on biological hydrogen production from palm oil mill effluent by dark fermentation using response surface methodology
topic QD Chemistry
TA Engineering (General). Civil engineering (General)
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