Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE)
The effects of Phe-Met-Arg-Phe-NH2 (FMRF-amide) and its analogue Phe-Leu-Arg-Phe-NH2 (FLRF-amide) on the nervous system were studied on thirtyfour identified neurons of the snail Achatina fulica Ferussac (locally known as "siput babi"). The results showed that FMRF-amide and FLRF-amide ind...
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Faculty of Medicine, University of Malaya
1996
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| author | Kim, Kah Hwi Muhamad, M.A. Cheah, Swee Hung Raman, A. |
| author_facet | Kim, Kah Hwi Muhamad, M.A. Cheah, Swee Hung Raman, A. |
| author_sort | Kim, Kah Hwi |
| collection | UM |
| description | The effects of Phe-Met-Arg-Phe-NH2 (FMRF-amide) and its analogue Phe-Leu-Arg-Phe-NH2 (FLRF-amide) on the nervous system were studied on thirtyfour identified neurons of the snail Achatina fulica Ferussac (locally known as "siput babi"). The results showed that FMRF-amide and FLRF-amide induced three different types of responses on the membrane potential of these neurons. First, FMRF-amide and FLRF-amide induced slow hyperpolarizing responses in TAN, d-LCDN, RAPN, d-LPeCN and V-RPLN. These responses remained unchanged in Cl--free, Ca2+ -free and 20% Na+ saline. The hyperpolarization was slightly reduced in 200% K+ and slightly enhanced in 50% K+ saline. Thus, the slow hyperpolarizing responses induced by FMRF-amide and FLRF-amide in the above neurons were K + -dependent. Second, FMRF-amide and FLRF-amide induced rapid and transient hyperpolarizing responses on RPeNLN, LPeNLN and TAN-2. The effect persisted in 200% K+, 20% Na+ and Ca2+ -free saline. However, in Cl--free saline, the effects were transient rapid depolarizing responses. This observation suggested that CI- was responsible for the hyperpolarizing responses. Third, FMRF-amide and FLRF-amide induced depolarizing responses in INN and this effect remained unchanged in Ca2+ -free saline. However, in 20% Na+ saline, the depolarizing responses were abolished. Thus, the involvement of Na+ was implicated in the observed depolarizing responses. The observed 3 different responses induced by FMRF-amide and FLRF-amide on Achatina fulica Ferussac neurons were comparable to their effects on the neurons of the gastropods Aplysia and Helix. Thus, FMRF-amide induced its multiple effects via multiple receptor sites, analogous to its action on Helix and Aplysia neurons |
| first_indexed | 2024-03-06T06:02:47Z |
| format | Article |
| id | um.eprints-24437 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:02:47Z |
| publishDate | 1996 |
| publisher | Faculty of Medicine, University of Malaya |
| record_format | dspace |
| spelling | um.eprints-244372020-06-02T02:53:35Z http://eprints.um.edu.my/24437/ Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) Kim, Kah Hwi Muhamad, M.A. Cheah, Swee Hung Raman, A. R Medicine The effects of Phe-Met-Arg-Phe-NH2 (FMRF-amide) and its analogue Phe-Leu-Arg-Phe-NH2 (FLRF-amide) on the nervous system were studied on thirtyfour identified neurons of the snail Achatina fulica Ferussac (locally known as "siput babi"). The results showed that FMRF-amide and FLRF-amide induced three different types of responses on the membrane potential of these neurons. First, FMRF-amide and FLRF-amide induced slow hyperpolarizing responses in TAN, d-LCDN, RAPN, d-LPeCN and V-RPLN. These responses remained unchanged in Cl--free, Ca2+ -free and 20% Na+ saline. The hyperpolarization was slightly reduced in 200% K+ and slightly enhanced in 50% K+ saline. Thus, the slow hyperpolarizing responses induced by FMRF-amide and FLRF-amide in the above neurons were K + -dependent. Second, FMRF-amide and FLRF-amide induced rapid and transient hyperpolarizing responses on RPeNLN, LPeNLN and TAN-2. The effect persisted in 200% K+, 20% Na+ and Ca2+ -free saline. However, in Cl--free saline, the effects were transient rapid depolarizing responses. This observation suggested that CI- was responsible for the hyperpolarizing responses. Third, FMRF-amide and FLRF-amide induced depolarizing responses in INN and this effect remained unchanged in Ca2+ -free saline. However, in 20% Na+ saline, the depolarizing responses were abolished. Thus, the involvement of Na+ was implicated in the observed depolarizing responses. The observed 3 different responses induced by FMRF-amide and FLRF-amide on Achatina fulica Ferussac neurons were comparable to their effects on the neurons of the gastropods Aplysia and Helix. Thus, FMRF-amide induced its multiple effects via multiple receptor sites, analogous to its action on Helix and Aplysia neurons Faculty of Medicine, University of Malaya 1996-12 Article PeerReviewed Kim, Kah Hwi and Muhamad, M.A. and Cheah, Swee Hung and Raman, A. (1996) Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE). Journal of the University of Malaya Medical Centre (JUMMEC), 1 (2). pp. 33-38. ISSN 1823-7339, https://jummec.um.edu.my/article/view/4387 |
| spellingShingle | R Medicine Kim, Kah Hwi Muhamad, M.A. Cheah, Swee Hung Raman, A. Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) |
| title | Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) |
| title_full | Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) |
| title_fullStr | Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) |
| title_full_unstemmed | Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) |
| title_short | Multiple receptor sites in Achatina fulica ferussac neurons for a molluscan tetrapeptide amide (FMRF-AMDE) |
| title_sort | multiple receptor sites in achatina fulica ferussac neurons for a molluscan tetrapeptide amide fmrf amde |
| topic | R Medicine |
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