Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol
Morphological changes in the hepatic microvasculature were studied in experimentally induced ischemia-reperfusion injury in the rat using a vascular casting technique. Partial hepatic ischemia was induced for 90 min followed by 24 hr reperfusion. Microvascular casting was performed after 24 hr reper...
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Faculty of Medicine, University of Malaya
1996
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| author | Lim, S.P. Andrews, F.J. Christophil, C. O'Brien, P.E. |
| author_facet | Lim, S.P. Andrews, F.J. Christophil, C. O'Brien, P.E. |
| author_sort | Lim, S.P. |
| collection | UM |
| description | Morphological changes in the hepatic microvasculature were studied in experimentally induced ischemia-reperfusion injury in the rat using a vascular casting technique. Partial hepatic ischemia was induced for 90 min followed by 24 hr reperfusion. Microvascular casting was performed after 24 hr reperfusion by either intraarterial or intravenous infusion of acrylic resin (Mercox). After corrosion of the tissue, the cast was examined by scanning electron microscopy. Casts of normal livers showed good patency with no evidence of unfilled areas. The mean diameter of sinusoids was 14 +/- 3 microns with those in zone 1 slightly smaller than those in zone 3. Liver casts from rats subjected to ischemia and reperfusion resulted in gross disruption of normal architecture. The common characteristics seen in both prograde and retrograde casts were clusters of closed sinusoids around zones 2 and 3 of the liver acini, which resulted in cavities of various sizes. Varicosities were observed in some areas. The mean diameter of sinusoids in areas of patent microvascular structure (10 +/- 2 microns) was significantly smaller compared to those in normal livers (P < 0.001). Misoprostol given at 1 min before reperfusion markedly reduced the microvascular injury. The hepatic microvascular was generally intact with mild focal unfilled areas. The majority of the sinusoids were of normal size and no clusters of blind ending sinusoids were detected. The present study shows that hepatic ischemia-reperfusion results in extensive microvascular injury in the liver. The protective effects of misoprostol against this injury may occur at the vascular level. |
| first_indexed | 2024-03-06T06:02:53Z |
| format | Article |
| id | um.eprints-24480 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:02:53Z |
| publishDate | 1996 |
| publisher | Faculty of Medicine, University of Malaya |
| record_format | dspace |
| spelling | um.eprints-244802021-03-24T01:52:47Z http://eprints.um.edu.my/24480/ Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol Lim, S.P. Andrews, F.J. Christophil, C. O'Brien, P.E. R Medicine Morphological changes in the hepatic microvasculature were studied in experimentally induced ischemia-reperfusion injury in the rat using a vascular casting technique. Partial hepatic ischemia was induced for 90 min followed by 24 hr reperfusion. Microvascular casting was performed after 24 hr reperfusion by either intraarterial or intravenous infusion of acrylic resin (Mercox). After corrosion of the tissue, the cast was examined by scanning electron microscopy. Casts of normal livers showed good patency with no evidence of unfilled areas. The mean diameter of sinusoids was 14 +/- 3 microns with those in zone 1 slightly smaller than those in zone 3. Liver casts from rats subjected to ischemia and reperfusion resulted in gross disruption of normal architecture. The common characteristics seen in both prograde and retrograde casts were clusters of closed sinusoids around zones 2 and 3 of the liver acini, which resulted in cavities of various sizes. Varicosities were observed in some areas. The mean diameter of sinusoids in areas of patent microvascular structure (10 +/- 2 microns) was significantly smaller compared to those in normal livers (P < 0.001). Misoprostol given at 1 min before reperfusion markedly reduced the microvascular injury. The hepatic microvascular was generally intact with mild focal unfilled areas. The majority of the sinusoids were of normal size and no clusters of blind ending sinusoids were detected. The present study shows that hepatic ischemia-reperfusion results in extensive microvascular injury in the liver. The protective effects of misoprostol against this injury may occur at the vascular level. Faculty of Medicine, University of Malaya 1996-12 Article PeerReviewed Lim, S.P. and Andrews, F.J. and Christophil, C. and O'Brien, P.E. (1996) Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol. Journal of the University of Malaya Medical Centre (JUMMEC), 1 (2). p. 57. ISSN 1823-7339, https://jummec.um.edu.my/issue/view/661/Vol.1%2C%20No.2%2C%201996 |
| spellingShingle | R Medicine Lim, S.P. Andrews, F.J. Christophil, C. O'Brien, P.E. Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol |
| title | Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol |
| title_full | Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol |
| title_fullStr | Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol |
| title_full_unstemmed | Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol |
| title_short | Microvascular changes in liver after ischemia-reperfusion injury: Protection with misoprostol |
| title_sort | microvascular changes in liver after ischemia reperfusion injury protection with misoprostol |
| topic | R Medicine |
| work_keys_str_mv | AT limsp microvascularchangesinliverafterischemiareperfusioninjuryprotectionwithmisoprostol AT andrewsfj microvascularchangesinliverafterischemiareperfusioninjuryprotectionwithmisoprostol AT christophilc microvascularchangesinliverafterischemiareperfusioninjuryprotectionwithmisoprostol AT obrienpe microvascularchangesinliverafterischemiareperfusioninjuryprotectionwithmisoprostol |