Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation
In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and...
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| Format: | Article |
| Language: | English |
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Springer Verlag (Germany)
2011
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| Online Access: | http://eprints.um.edu.my/2457/1/molecules-16-02583.pdf |
| _version_ | 1825718681495142400 |
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| author | Cheah, S.C. Appleton, D.R. Lee, S.T. Lam, M.L. Hadi, A.H.A. Mustafa, Mohd Rais |
| author_facet | Cheah, S.C. Appleton, D.R. Lee, S.T. Lam, M.L. Hadi, A.H.A. Mustafa, Mohd Rais |
| author_sort | Cheah, S.C. |
| collection | UM |
| description | In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL 10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factoralpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line,suggesting its therapeutic potential as an NF-κB inhibitor. |
| first_indexed | 2024-03-06T05:08:06Z |
| format | Article |
| id | um.eprints-2457 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:08:06Z |
| publishDate | 2011 |
| publisher | Springer Verlag (Germany) |
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| spelling | um.eprints-24572019-12-18T06:30:31Z http://eprints.um.edu.my/2457/ Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation Cheah, S.C. Appleton, D.R. Lee, S.T. Lam, M.L. Hadi, A.H.A. Mustafa, Mohd Rais QD Chemistry R Medicine In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL 10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factoralpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line,suggesting its therapeutic potential as an NF-κB inhibitor. Springer Verlag (Germany) 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2457/1/molecules-16-02583.pdf Cheah, S.C. and Appleton, D.R. and Lee, S.T. and Lam, M.L. and Hadi, A.H.A. and Mustafa, Mohd Rais (2011) Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation. Molecules, 16 (3). pp. 2583-2598. |
| spellingShingle | QD Chemistry R Medicine Cheah, S.C. Appleton, D.R. Lee, S.T. Lam, M.L. Hadi, A.H.A. Mustafa, Mohd Rais Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation |
| title | Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation |
| title_full | Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation |
| title_fullStr | Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation |
| title_full_unstemmed | Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation |
| title_short | Panduratin a inhibits the growth of A549 Cells through induction of apoptosis and inhibition of NF-KappaB translocation |
| title_sort | panduratin a inhibits the growth of a549 cells through induction of apoptosis and inhibition of nf kappab translocation |
| topic | QD Chemistry R Medicine |
| url | http://eprints.um.edu.my/2457/1/molecules-16-02583.pdf |
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