Cellular Localization of gdnf in Adult Zebrafish Brain
Glial cell line-derived neurotrophic factor (GDNF) was initially described as important for dopaminergic neuronal survival and is involved in many other essential functions in the central nervous system. Characterization of GDNF phenotype in mammals is well described; however, studies in non-mammali...
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| Format: | Article |
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MDPI
2020
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| _version_ | 1825722307843194880 |
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| author | Wong, Chee Ern David Hua, Khang Monis, Simon Norazit, Anwar Noor, Suzita Mohd Ekker, Marc |
| author_facet | Wong, Chee Ern David Hua, Khang Monis, Simon Norazit, Anwar Noor, Suzita Mohd Ekker, Marc |
| author_sort | Wong, Chee Ern David |
| collection | UM |
| description | Glial cell line-derived neurotrophic factor (GDNF) was initially described as important for dopaminergic neuronal survival and is involved in many other essential functions in the central nervous system. Characterization of GDNF phenotype in mammals is well described; however, studies in non-mammalian vertebrate models are scarce. Here, we characterized the anatomical distribution of gdnf -expressing cells in adult zebrafish brain by means of combined in situ hybridization (ISH) and immunohistochemistry. Our results revealed that gdnf was widely dispersed in the brain. gdnf transcripts were co-localized with radial glial cells along the ventricular area of the telencephalon and in the hypothalamus. Interestingly, Sox2 positive cells expressed gdnf in the neuronal layer but not in the ventricular zone of the telencephalon. A subset of GABAergic precursor cells labeled with dlx6a-1.4kbdlx5a/6a: green fluorescence protein (GFP) in the pallium, parvocellular preoptic nucleus, and the anterior and dorsal zones of the periventricular hypothalamus also showed expression with gdnf mRNA. In addition, gdnf signals were detected in subsets of dopaminergic neurons, including those in the ventral diencephalon, similar to what is seen in mammalian brain. Our work extends our knowledge of gdnf action sites and suggests a potential role for gdnf in adult brain neurogenesis and regeneration. © 2020 by the authors. |
| first_indexed | 2024-03-06T06:03:41Z |
| format | Article |
| id | um.eprints-24761 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:03:41Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | um.eprints-247612020-06-10T00:35:54Z http://eprints.um.edu.my/24761/ Cellular Localization of gdnf in Adult Zebrafish Brain Wong, Chee Ern David Hua, Khang Monis, Simon Norazit, Anwar Noor, Suzita Mohd Ekker, Marc QH Natural history R Medicine Glial cell line-derived neurotrophic factor (GDNF) was initially described as important for dopaminergic neuronal survival and is involved in many other essential functions in the central nervous system. Characterization of GDNF phenotype in mammals is well described; however, studies in non-mammalian vertebrate models are scarce. Here, we characterized the anatomical distribution of gdnf -expressing cells in adult zebrafish brain by means of combined in situ hybridization (ISH) and immunohistochemistry. Our results revealed that gdnf was widely dispersed in the brain. gdnf transcripts were co-localized with radial glial cells along the ventricular area of the telencephalon and in the hypothalamus. Interestingly, Sox2 positive cells expressed gdnf in the neuronal layer but not in the ventricular zone of the telencephalon. A subset of GABAergic precursor cells labeled with dlx6a-1.4kbdlx5a/6a: green fluorescence protein (GFP) in the pallium, parvocellular preoptic nucleus, and the anterior and dorsal zones of the periventricular hypothalamus also showed expression with gdnf mRNA. In addition, gdnf signals were detected in subsets of dopaminergic neurons, including those in the ventral diencephalon, similar to what is seen in mammalian brain. Our work extends our knowledge of gdnf action sites and suggests a potential role for gdnf in adult brain neurogenesis and regeneration. © 2020 by the authors. MDPI 2020 Article PeerReviewed Wong, Chee Ern David and Hua, Khang and Monis, Simon and Norazit, Anwar and Noor, Suzita Mohd and Ekker, Marc (2020) Cellular Localization of gdnf in Adult Zebrafish Brain. Brain Sciences, 10 (5). p. 286. ISSN 2076-3425, DOI https://doi.org/10.3390/brainsci10050286 <https://doi.org/10.3390/brainsci10050286>. https://doi.org/10.3390/brainsci10050286 doi:10.3390/brainsci10050286 |
| spellingShingle | QH Natural history R Medicine Wong, Chee Ern David Hua, Khang Monis, Simon Norazit, Anwar Noor, Suzita Mohd Ekker, Marc Cellular Localization of gdnf in Adult Zebrafish Brain |
| title | Cellular Localization of gdnf in Adult Zebrafish Brain |
| title_full | Cellular Localization of gdnf in Adult Zebrafish Brain |
| title_fullStr | Cellular Localization of gdnf in Adult Zebrafish Brain |
| title_full_unstemmed | Cellular Localization of gdnf in Adult Zebrafish Brain |
| title_short | Cellular Localization of gdnf in Adult Zebrafish Brain |
| title_sort | cellular localization of gdnf in adult zebrafish brain |
| topic | QH Natural history R Medicine |
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