Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo
Natural mycelial biomass (MB) and extracted exopolysaccharide (EPS) from the pre-grown Malaysian Ganoderma lucidum mushroom are both considered as high-end materials due to their high commercial value in the aquaculture industry. To evaluate their potential toxicity as a fish-feed supplement for the...
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Elsevier
2020
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| _version_ | 1796962098222202880 |
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| author | Taufek, Norhidayah Mohd Harith, Hanis H. Abd Rahim, Muhamad Hafiz Ilham, Zul Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad |
| author_facet | Taufek, Norhidayah Mohd Harith, Hanis H. Abd Rahim, Muhamad Hafiz Ilham, Zul Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad |
| author_sort | Taufek, Norhidayah Mohd |
| collection | UM |
| description | Natural mycelial biomass (MB) and extracted exopolysaccharide (EPS) from the pre-grown Malaysian Ganoderma lucidum mushroom are both considered as high-end materials due to their high commercial value in the aquaculture industry. To evaluate their potential toxicity as a fish-feed supplement for the fungivore red hybrid Tilapia (Oreochromis sp.), both MB (250−5000 μg/mL) and EPS (62.5−3000 μg/mL) were subjected to zebrafish embryo toxicity (ZFET) assay, and the effects on zebrafish embryos (ZE) early development were analyzed between 24−120 hours of post-exposure (HPE). MB and EPS showed no toxic effect towards the ZE with LC50 of 1650 μg/mL and 2648.38 μg/mL, respectively. MB at concentrations between 250−5000 μg/mL and EPS at 3000 μg/mL showed no significant changes in ZE hatching. No significant changes in the ZE heart rate were detected following treatment with both tested compounds (MB: 250−2000 μg/mL and EPS: 62.5−3000 μg/mL) as compared to untreated embryos (135.5 beats/min). Furthermore, teratogenic effects of both MB and EPS (<3000 μg/mL) on zebrafish embryonic development were not observed. Together, both natural compounds MB and EPS can be considered non-toxic, suggesting that these can be safely applied as feed substances in the fish-feed aquaculture industry. © 2020 The Authors |
| first_indexed | 2024-03-06T06:03:51Z |
| format | Article |
| id | um.eprints-24817 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:03:51Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-248172020-06-15T02:29:43Z http://eprints.um.edu.my/24817/ Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo Taufek, Norhidayah Mohd Harith, Hanis H. Abd Rahim, Muhamad Hafiz Ilham, Zul Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad Q Science (General) QH Natural history Natural mycelial biomass (MB) and extracted exopolysaccharide (EPS) from the pre-grown Malaysian Ganoderma lucidum mushroom are both considered as high-end materials due to their high commercial value in the aquaculture industry. To evaluate their potential toxicity as a fish-feed supplement for the fungivore red hybrid Tilapia (Oreochromis sp.), both MB (250−5000 μg/mL) and EPS (62.5−3000 μg/mL) were subjected to zebrafish embryo toxicity (ZFET) assay, and the effects on zebrafish embryos (ZE) early development were analyzed between 24−120 hours of post-exposure (HPE). MB and EPS showed no toxic effect towards the ZE with LC50 of 1650 μg/mL and 2648.38 μg/mL, respectively. MB at concentrations between 250−5000 μg/mL and EPS at 3000 μg/mL showed no significant changes in ZE hatching. No significant changes in the ZE heart rate were detected following treatment with both tested compounds (MB: 250−2000 μg/mL and EPS: 62.5−3000 μg/mL) as compared to untreated embryos (135.5 beats/min). Furthermore, teratogenic effects of both MB and EPS (<3000 μg/mL) on zebrafish embryonic development were not observed. Together, both natural compounds MB and EPS can be considered non-toxic, suggesting that these can be safely applied as feed substances in the fish-feed aquaculture industry. © 2020 The Authors Elsevier 2020 Article PeerReviewed Taufek, Norhidayah Mohd and Harith, Hanis H. and Abd Rahim, Muhamad Hafiz and Ilham, Zul and Rowan, Neil and Wan-Mohtar, Wan Abd Al Qadr Imad (2020) Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo. Aquaculture Reports, 17. p. 100322. ISSN 2352-5134, DOI https://doi.org/10.1016/j.aqrep.2020.100322 <https://doi.org/10.1016/j.aqrep.2020.100322>. https://doi.org/10.1016/j.aqrep.2020.100322 doi:10.1016/j.aqrep.2020.100322 |
| spellingShingle | Q Science (General) QH Natural history Taufek, Norhidayah Mohd Harith, Hanis H. Abd Rahim, Muhamad Hafiz Ilham, Zul Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo |
| title | Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo |
| title_full | Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo |
| title_fullStr | Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo |
| title_full_unstemmed | Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo |
| title_short | Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo |
| title_sort | performance of mycelial biomass and exopolysaccharide from malaysian ganoderma lucidum for the fungivore red hybrid tilapia oreochromis sp in zebrafish embryo |
| topic | Q Science (General) QH Natural history |
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