TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements
Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan pro...
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Elsevier
2020
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| author | Sultana, Sharmin Hossain, M.A. Motalib Azlan, Azrina Johan, Mohd Rafie Chowdhury, Zaira Zaman Ali, Md. Eaqub |
| author_facet | Sultana, Sharmin Hossain, M.A. Motalib Azlan, Azrina Johan, Mohd Rafie Chowdhury, Zaira Zaman Ali, Md. Eaqub |
| author_sort | Sultana, Sharmin |
| collection | UM |
| description | Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform. The assay was specific to cattle, pigs and fish, having been tested against 14 non-target species. The limit of detection, under gelatin admixed conditions, was 0.005 ng/µL. Finally, a pilot survey was undertaken testing 35 Halal branded processed food and dietary items. Out of 35 samples, only two were found to be positive for porcine species. The authenticity of these two qPCR products was confirmed by DNA sequencing analysis, which showed 99–100% similarity with Sus scrofa (Wild boar) species. © 2020 Elsevier Ltd |
| first_indexed | 2024-03-06T06:03:59Z |
| format | Article |
| id | um.eprints-24864 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T06:03:59Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-248642020-06-17T00:57:05Z http://eprints.um.edu.my/24864/ TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements Sultana, Sharmin Hossain, M.A. Motalib Azlan, Azrina Johan, Mohd Rafie Chowdhury, Zaira Zaman Ali, Md. Eaqub QH Natural history S Agriculture (General) Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform. The assay was specific to cattle, pigs and fish, having been tested against 14 non-target species. The limit of detection, under gelatin admixed conditions, was 0.005 ng/µL. Finally, a pilot survey was undertaken testing 35 Halal branded processed food and dietary items. Out of 35 samples, only two were found to be positive for porcine species. The authenticity of these two qPCR products was confirmed by DNA sequencing analysis, which showed 99–100% similarity with Sus scrofa (Wild boar) species. © 2020 Elsevier Ltd Elsevier 2020 Article PeerReviewed Sultana, Sharmin and Hossain, M.A. Motalib and Azlan, Azrina and Johan, Mohd Rafie and Chowdhury, Zaira Zaman and Ali, Md. Eaqub (2020) TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements. Food Chemistry, 325. p. 126756. ISSN 0308-8146, DOI https://doi.org/10.1016/j.foodchem.2020.126756 <https://doi.org/10.1016/j.foodchem.2020.126756>. https://doi.org/10.1016/j.foodchem.2020.126756 doi:10.1016/j.foodchem.2020.126756 |
| spellingShingle | QH Natural history S Agriculture (General) Sultana, Sharmin Hossain, M.A. Motalib Azlan, Azrina Johan, Mohd Rafie Chowdhury, Zaira Zaman Ali, Md. Eaqub TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements |
| title | TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements |
| title_full | TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements |
| title_fullStr | TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements |
| title_full_unstemmed | TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements |
| title_short | TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements |
| title_sort | taqman probe based multiplex quantitative pcr assay for determination of bovine porcine and fish dna in gelatin admixture food products and dietary supplements |
| topic | QH Natural history S Agriculture (General) |
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