Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia

Introduction. Burkholderia pseudomallei (melioidosis) is an important cause of community-acquired pneumonia (CAP) in the tropics. Selective medium is recommended for laboratory diagnosis with non-sterile respiratory samples, while PCR is not routinely used due to variable reported performance. The e...

Full description

Bibliographic Details
Main Authors: Subakir, Haida, Chong, Yoong Min, Chan, Yoke Fun, Hasan, Mohd Shahnaz, Jamaluddin, Mohamad Fadhil Hadi, Pang, Yong Kek, Ponnampalavanar, Sasheela, Syed Omar, Sharifah Faridah, Sam, I-Ching
Format: Article
Published: Microbiology Society 2020
Subjects:
_version_ 1825722330529136640
author Subakir, Haida
Chong, Yoong Min
Chan, Yoke Fun
Hasan, Mohd Shahnaz
Jamaluddin, Mohamad Fadhil Hadi
Pang, Yong Kek
Ponnampalavanar, Sasheela
Syed Omar, Sharifah Faridah
Sam, I-Ching
author_facet Subakir, Haida
Chong, Yoong Min
Chan, Yoke Fun
Hasan, Mohd Shahnaz
Jamaluddin, Mohamad Fadhil Hadi
Pang, Yong Kek
Ponnampalavanar, Sasheela
Syed Omar, Sharifah Faridah
Sam, I-Ching
author_sort Subakir, Haida
collection UM
description Introduction. Burkholderia pseudomallei (melioidosis) is an important cause of community-acquired pneumonia (CAP) in the tropics. Selective medium is recommended for laboratory diagnosis with non-sterile respiratory samples, while PCR is not routinely used due to variable reported performance. The effectiveness of these diagnostic modalities varies by site. Aim. To compare selective media and real-time PCR (qPCR) with routine media in detecting B. pseudomallei in CAP respiratory samples in a low-incidence setting in Kuala Lumpur, Malaysia. Methodology. Respiratory samples were routinely cultured on blood, chocolate and MacConkey agar (RESP-ROUTINE), and compared to culture on selective Ashdown medium (RESP-SELECTIVE) and qPCR. The gold standard was routine culture of B. pseudomallei from any site (ALL-ROUTINE). Results. B. pseudomallei was detected in 8/204 (3.9%) samples. Overall sensitivity rates differed (P=0.03) for qPCR (100%), RESP-SELECTIVE (87.5%) and RESP-ROUTINE (50%). There was a trend towards lower median days to positive culture for RESP-SELECTIVE (1 day) compared to RESP-ROUTINE (2 days, P=0.08) and ALL-ROUTINE (2 days, P=0.06). Reagent costs for each additional detection were USD59 for RESP-SELECTIVE and USD354 for PCR. Conclusions. In a low-incidence setting, selective culture of respiratory samples on Ashdown was more sensitive and allowed quicker identification than routine media, at reasonable cost. Blood cultures are critical, confirming four cases missed by routine respiratory culture. Selective medium is useful in early pneumonia (pre-sepsis) and resource-limited settings where blood cultures are infrequently done. Real-time PCR is costly, but highly sensitive and useful for high-risk patients with diabetes, cancer or immunosuppressants, or requiring ventilation or intensive care. © 2020 The Authors
first_indexed 2024-03-06T06:04:01Z
format Article
id um.eprints-24875
institution Universiti Malaya
last_indexed 2024-03-06T06:04:01Z
publishDate 2020
publisher Microbiology Society
record_format dspace
spelling um.eprints-248752020-06-17T02:11:20Z http://eprints.um.edu.my/24875/ Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia Subakir, Haida Chong, Yoong Min Chan, Yoke Fun Hasan, Mohd Shahnaz Jamaluddin, Mohamad Fadhil Hadi Pang, Yong Kek Ponnampalavanar, Sasheela Syed Omar, Sharifah Faridah Sam, I-Ching R Medicine Introduction. Burkholderia pseudomallei (melioidosis) is an important cause of community-acquired pneumonia (CAP) in the tropics. Selective medium is recommended for laboratory diagnosis with non-sterile respiratory samples, while PCR is not routinely used due to variable reported performance. The effectiveness of these diagnostic modalities varies by site. Aim. To compare selective media and real-time PCR (qPCR) with routine media in detecting B. pseudomallei in CAP respiratory samples in a low-incidence setting in Kuala Lumpur, Malaysia. Methodology. Respiratory samples were routinely cultured on blood, chocolate and MacConkey agar (RESP-ROUTINE), and compared to culture on selective Ashdown medium (RESP-SELECTIVE) and qPCR. The gold standard was routine culture of B. pseudomallei from any site (ALL-ROUTINE). Results. B. pseudomallei was detected in 8/204 (3.9%) samples. Overall sensitivity rates differed (P=0.03) for qPCR (100%), RESP-SELECTIVE (87.5%) and RESP-ROUTINE (50%). There was a trend towards lower median days to positive culture for RESP-SELECTIVE (1 day) compared to RESP-ROUTINE (2 days, P=0.08) and ALL-ROUTINE (2 days, P=0.06). Reagent costs for each additional detection were USD59 for RESP-SELECTIVE and USD354 for PCR. Conclusions. In a low-incidence setting, selective culture of respiratory samples on Ashdown was more sensitive and allowed quicker identification than routine media, at reasonable cost. Blood cultures are critical, confirming four cases missed by routine respiratory culture. Selective medium is useful in early pneumonia (pre-sepsis) and resource-limited settings where blood cultures are infrequently done. Real-time PCR is costly, but highly sensitive and useful for high-risk patients with diabetes, cancer or immunosuppressants, or requiring ventilation or intensive care. © 2020 The Authors Microbiology Society 2020 Article PeerReviewed Subakir, Haida and Chong, Yoong Min and Chan, Yoke Fun and Hasan, Mohd Shahnaz and Jamaluddin, Mohamad Fadhil Hadi and Pang, Yong Kek and Ponnampalavanar, Sasheela and Syed Omar, Sharifah Faridah and Sam, I-Ching (2020) Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia. Journal of Medical Microbiology, 69 (1). pp. 49-51. ISSN 0022-2615, DOI https://doi.org/10.1099/jmm.0.001108 <https://doi.org/10.1099/jmm.0.001108>. https://doi.org/10.1099/jmm.0.001108 doi:10.1099/jmm.0.001108
spellingShingle R Medicine
Subakir, Haida
Chong, Yoong Min
Chan, Yoke Fun
Hasan, Mohd Shahnaz
Jamaluddin, Mohamad Fadhil Hadi
Pang, Yong Kek
Ponnampalavanar, Sasheela
Syed Omar, Sharifah Faridah
Sam, I-Ching
Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia
title Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia
title_full Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia
title_fullStr Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia
title_full_unstemmed Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia
title_short Selective media and real-time PCR improves diagnosis of melioidosis in community-acquired pneumonia in a low-incidence setting in Kuala Lumpur, Malaysia
title_sort selective media and real time pcr improves diagnosis of melioidosis in community acquired pneumonia in a low incidence setting in kuala lumpur malaysia
topic R Medicine
work_keys_str_mv AT subakirhaida selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT chongyoongmin selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT chanyokefun selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT hasanmohdshahnaz selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT jamaluddinmohamadfadhilhadi selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT pangyongkek selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT ponnampalavanarsasheela selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT syedomarsharifahfaridah selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia
AT samiching selectivemediaandrealtimepcrimprovesdiagnosisofmelioidosisincommunityacquiredpneumoniainalowincidencesettinginkualalumpurmalaysia