Complete genome sequence analysis of dengue virus type 2 isolated in Brunei
In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 vi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2008
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/2734/1/Complete_genome_sequence_analysis_of_dengue_virus_type_2_isolated_in_Brunei.pdf |
| _version_ | 1796944901528616960 |
|---|---|
| author | Osman, O. Fong, M.Y. Devi, S. |
| author_facet | Osman, O. Fong, M.Y. Devi, S. |
| author_sort | Osman, O. |
| collection | UM |
| description | In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005-2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleoticles and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains. (c) 2008 Elsevier B.V. All rights reserved. |
| first_indexed | 2024-03-06T05:08:38Z |
| format | Article |
| id | um.eprints-2734 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:08:38Z |
| publishDate | 2008 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-27342014-12-21T09:37:30Z http://eprints.um.edu.my/2734/ Complete genome sequence analysis of dengue virus type 2 isolated in Brunei Osman, O. Fong, M.Y. Devi, S. R Medicine In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005-2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleoticles and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains. (c) 2008 Elsevier B.V. All rights reserved. Elsevier 2008 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2734/1/Complete_genome_sequence_analysis_of_dengue_virus_type_2_isolated_in_Brunei.pdf Osman, O. and Fong, M.Y. and Devi, S. (2008) Complete genome sequence analysis of dengue virus type 2 isolated in Brunei. Virus Research, 135 (1). pp. 48-52. ISSN 0168-1702, DOI https://doi.org/10.1016/j.virusres.2008.02.006 <https://doi.org/10.1016/j.virusres.2008.02.006>. http://www.sciencedirect.com/science/article/pii/S016817020800066X 10.1016/j.virusres.2008.02.006 |
| spellingShingle | R Medicine Osman, O. Fong, M.Y. Devi, S. Complete genome sequence analysis of dengue virus type 2 isolated in Brunei |
| title | Complete genome sequence analysis of dengue virus type 2 isolated in Brunei |
| title_full | Complete genome sequence analysis of dengue virus type 2 isolated in Brunei |
| title_fullStr | Complete genome sequence analysis of dengue virus type 2 isolated in Brunei |
| title_full_unstemmed | Complete genome sequence analysis of dengue virus type 2 isolated in Brunei |
| title_short | Complete genome sequence analysis of dengue virus type 2 isolated in Brunei |
| title_sort | complete genome sequence analysis of dengue virus type 2 isolated in brunei |
| topic | R Medicine |
| url | http://eprints.um.edu.my/2734/1/Complete_genome_sequence_analysis_of_dengue_virus_type_2_isolated_in_Brunei.pdf |
| work_keys_str_mv | AT osmano completegenomesequenceanalysisofdenguevirustype2isolatedinbrunei AT fongmy completegenomesequenceanalysisofdenguevirustype2isolatedinbrunei AT devis completegenomesequenceanalysisofdenguevirustype2isolatedinbrunei |