Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris
The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. The entire recombinant human erythropoietin (rhEPO) gene was constructed using the Splicing by Overla...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2011
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| Online Access: | http://eprints.um.edu.my/2738/1/Expression_and_analysis_of_the_glycosylation_properties_of_recombinant_human_erythropoietin_expressed_in_Pichia_pastoris.pdf |
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| author | Teh, Ser Huy Fong, Mun Yik Mohamed, Zulqarnain |
| author_facet | Teh, Ser Huy Fong, Mun Yik Mohamed, Zulqarnain |
| author_sort | Teh, Ser Huy |
| collection | UM |
| description | The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. The entire recombinant human erythropoietin (rhEPO) gene was constructed using the Splicing by Overlap Extension by PCR (SOE-PCR) technique, cloned and expressed through the secretory pathway of the Pichia expression system. Recombinant erythropoietin was successfully expressed in P. pastoris. The estimated molecular mass of the expressed protein ranged from 32 kDa to 75 kDa, with the variation in size being attributed to the presence of rhEPO glycosylation analogs. A crude functional analysis of the soluble proteins showed that all of the forms were active in vivo. |
| first_indexed | 2024-03-06T05:08:39Z |
| format | Article |
| id | um.eprints-2738 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:08:39Z |
| publishDate | 2011 |
| publisher | Sociedade Brasileira de Genética |
| record_format | dspace |
| spelling | um.eprints-27382019-11-18T08:15:48Z http://eprints.um.edu.my/2738/ Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris Teh, Ser Huy Fong, Mun Yik Mohamed, Zulqarnain R Medicine The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. The entire recombinant human erythropoietin (rhEPO) gene was constructed using the Splicing by Overlap Extension by PCR (SOE-PCR) technique, cloned and expressed through the secretory pathway of the Pichia expression system. Recombinant erythropoietin was successfully expressed in P. pastoris. The estimated molecular mass of the expressed protein ranged from 32 kDa to 75 kDa, with the variation in size being attributed to the presence of rhEPO glycosylation analogs. A crude functional analysis of the soluble proteins showed that all of the forms were active in vivo. Sociedade Brasileira de Genética 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/2738/1/Expression_and_analysis_of_the_glycosylation_properties_of_recombinant_human_erythropoietin_expressed_in_Pichia_pastoris.pdf Teh, Ser Huy and Fong, Mun Yik and Mohamed, Zulqarnain (2011) Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris. Genetics and Molecular Biology, 34 (3). pp. 464-470. ISSN 1415-4757, DOI https://doi.org/10.1590/S1415-47572011005000022 <https://doi.org/10.1590/S1415-47572011005000022>. http://dx.doi.org/10.1590/S1415-47572011005000022 doi:10.1590/S1415-47572011005000022 |
| spellingShingle | R Medicine Teh, Ser Huy Fong, Mun Yik Mohamed, Zulqarnain Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris |
| title | Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris |
| title_full | Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris |
| title_fullStr | Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris |
| title_full_unstemmed | Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris |
| title_short | Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris |
| title_sort | expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in pichia pastoris |
| topic | R Medicine |
| url | http://eprints.um.edu.my/2738/1/Expression_and_analysis_of_the_glycosylation_properties_of_recombinant_human_erythropoietin_expressed_in_Pichia_pastoris.pdf |
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