Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis
Rhoptry protein 2 (ROP2) of Toxoplasma gondii is a rhoptry-secreted protein that plays a critical role in parasitophorous vacuole membrane formation during invasion. In previous studies, ROP2 has been shown to be efficient in triggering humoral and cell-mediated responses. High immunogenicity of ROP...
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| Format: | Article |
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2011
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| author | Chang, P.Y. Fong, M.Y. Nissapatorn, V. Lau, Y.L. |
| author_facet | Chang, P.Y. Fong, M.Y. Nissapatorn, V. Lau, Y.L. |
| author_sort | Chang, P.Y. |
| collection | UM |
| description | Rhoptry protein 2 (ROP2) of Toxoplasma gondii is a rhoptry-secreted protein that plays a critical role in parasitophorous vacuole membrane formation during invasion. In previous studies, ROP2 has been shown to be efficient in triggering humoral and cell-mediated responses. High immunogenicity of ROP2 makes it a potential candidate for diagnosis and vaccination against toxoplasmosis. In this study, the ROP2 gene was cloned into pPICZ alpha A expression vector and extracellularly expressed in the yeast Pichia pastoris, which has numerous advantages over other expression systems for eukaryotic proteins expression. The effectiveness of the secreted recombinant ROP2 as a diagnosis agent was assessed by Western Blot with 200 human serum samples. Recombinant ROP2 reacted with toxoplasmosis-positive human serum samples and yielded an overall sensitivity of 90 and specificity of 95. However, recombinant ROP2 is a better marker for detection of IgG (91.7) rather than IgM (80). |
| first_indexed | 2024-03-06T05:08:40Z |
| format | Article |
| id | um.eprints-2743 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:08:40Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | um.eprints-27432014-12-16T04:21:44Z http://eprints.um.edu.my/2743/ Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis Chang, P.Y. Fong, M.Y. Nissapatorn, V. Lau, Y.L. R Medicine Rhoptry protein 2 (ROP2) of Toxoplasma gondii is a rhoptry-secreted protein that plays a critical role in parasitophorous vacuole membrane formation during invasion. In previous studies, ROP2 has been shown to be efficient in triggering humoral and cell-mediated responses. High immunogenicity of ROP2 makes it a potential candidate for diagnosis and vaccination against toxoplasmosis. In this study, the ROP2 gene was cloned into pPICZ alpha A expression vector and extracellularly expressed in the yeast Pichia pastoris, which has numerous advantages over other expression systems for eukaryotic proteins expression. The effectiveness of the secreted recombinant ROP2 as a diagnosis agent was assessed by Western Blot with 200 human serum samples. Recombinant ROP2 reacted with toxoplasmosis-positive human serum samples and yielded an overall sensitivity of 90 and specificity of 95. However, recombinant ROP2 is a better marker for detection of IgG (91.7) rather than IgM (80). 2011 Article PeerReviewed Chang, P.Y. and Fong, M.Y. and Nissapatorn, V. and Lau, Y.L. (2011) Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis. American Journal of Tropical Medicine and Hygiene, 85 (3). pp. 485-489. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.2011.11-0351 <https://doi.org/10.4269/ajtmh.2011.11-0351>. http://www.ajtmh.org/content/85/3/485.short 10.4269/ajtmh.2011.11-0351 |
| spellingShingle | R Medicine Chang, P.Y. Fong, M.Y. Nissapatorn, V. Lau, Y.L. Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis |
| title | Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis |
| title_full | Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis |
| title_fullStr | Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis |
| title_full_unstemmed | Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis |
| title_short | Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis |
| title_sort | evaluation of pichia pastoris expressed recombinant rhoptry protein 2 of toxoplasma gondii for its application in diagnosis of toxoplasmosis |
| topic | R Medicine |
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