Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV)
In this study, we have reported a full length of small heat shock protein 37 (designated MrHSP37) gene, identified from the transcriptome database of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrHSP37 is 2,425 base pairs in length, and encodes 338 amino acids. MrHS...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Kluwer (now part of Springer)
2012
|
| Subjects: |
| _version_ | 1796944919127916544 |
|---|---|
| author | Arockiaraj, J. Vanaraja, P. Easwvaran, S. Singh, A. Othman, R.Y. Bhassu, S. |
| author_facet | Arockiaraj, J. Vanaraja, P. Easwvaran, S. Singh, A. Othman, R.Y. Bhassu, S. |
| author_sort | Arockiaraj, J. |
| collection | UM |
| description | In this study, we have reported a full length of small heat shock protein 37 (designated MrHSP37) gene, identified from the transcriptome database of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrHSP37 is 2,425 base pairs in length, and encodes 338 amino acids. MrHSP37 contains a long heat shock protein family profile in the amino acid sequence between 205 and 288. The mRNA expressions of MrHSP37 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) challenged M. rosenbergii were examined using quantitative real time polymerase chain reaction (qRT-PCR). MrHSP37 is highly expressed in hepatopancreas and all the other tissues (walking leg, gills, muscle, stomach, haemocyte, intestine, pleopods, brain and eye stalk) of M. rosenbergii taken for analysis. The expression is strongly up-regulated after IHHNV challenge. To understand its biological activity, the recombinant MrHSP37 gene was constructed and expressed in Escherichia coli BL21 (DE3). The results of ATPase assay showed that the recombinant MrHSP37 protein exhibited apparent ATPase activity which increased with the concentration of the protein. And also the purified recombinant MrHSP37 protein was used for thermal aggregation assay (chaperone activity). It showed that the recombinant MrHSP37 protein is an active chaperone in this assay. Taken together, these results suggest that MrHSP37 is potentially involved in the immune responses against IHHNV challenge in M. rosenbergii. |
| first_indexed | 2024-03-06T05:08:54Z |
| format | Article |
| id | um.eprints-2936 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:08:54Z |
| publishDate | 2012 |
| publisher | Kluwer (now part of Springer) |
| record_format | dspace |
| spelling | um.eprints-29362013-12-11T02:41:31Z http://eprints.um.edu.my/2936/ Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) Arockiaraj, J. Vanaraja, P. Easwvaran, S. Singh, A. Othman, R.Y. Bhassu, S. R Medicine In this study, we have reported a full length of small heat shock protein 37 (designated MrHSP37) gene, identified from the transcriptome database of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrHSP37 is 2,425 base pairs in length, and encodes 338 amino acids. MrHSP37 contains a long heat shock protein family profile in the amino acid sequence between 205 and 288. The mRNA expressions of MrHSP37 in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) challenged M. rosenbergii were examined using quantitative real time polymerase chain reaction (qRT-PCR). MrHSP37 is highly expressed in hepatopancreas and all the other tissues (walking leg, gills, muscle, stomach, haemocyte, intestine, pleopods, brain and eye stalk) of M. rosenbergii taken for analysis. The expression is strongly up-regulated after IHHNV challenge. To understand its biological activity, the recombinant MrHSP37 gene was constructed and expressed in Escherichia coli BL21 (DE3). The results of ATPase assay showed that the recombinant MrHSP37 protein exhibited apparent ATPase activity which increased with the concentration of the protein. And also the purified recombinant MrHSP37 protein was used for thermal aggregation assay (chaperone activity). It showed that the recombinant MrHSP37 protein is an active chaperone in this assay. Taken together, these results suggest that MrHSP37 is potentially involved in the immune responses against IHHNV challenge in M. rosenbergii. Kluwer (now part of Springer) 2012-01-13 Article PeerReviewed Arockiaraj, J. and Vanaraja, P. and Easwvaran, S. and Singh, A. and Othman, R.Y. and Bhassu, S. (2012) Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV). Molecular Biology Reports. ISSN 0301-4851, DOI PMID: 22290288. http://www.ncbi.nlm.nih.gov/pubmed/22290288 PMID: 22290288 |
| spellingShingle | R Medicine Arockiaraj, J. Vanaraja, P. Easwvaran, S. Singh, A. Othman, R.Y. Bhassu, S. Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) |
| title | Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) |
| title_full | Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) |
| title_fullStr | Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) |
| title_full_unstemmed | Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) |
| title_short | Gene expression and functional studies of small heat shock protein 37 (MrHSP37) from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus (IHHNV) |
| title_sort | gene expression and functional studies of small heat shock protein 37 mrhsp37 from macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus ihhnv |
| topic | R Medicine |
| work_keys_str_mv | AT arockiarajj geneexpressionandfunctionalstudiesofsmallheatshockprotein37mrhsp37frommacrobrachiumrosenbergiichallengedwithinfectioushypodermalandhematopoieticnecrosisvirusihhnv AT vanarajap geneexpressionandfunctionalstudiesofsmallheatshockprotein37mrhsp37frommacrobrachiumrosenbergiichallengedwithinfectioushypodermalandhematopoieticnecrosisvirusihhnv AT easwvarans geneexpressionandfunctionalstudiesofsmallheatshockprotein37mrhsp37frommacrobrachiumrosenbergiichallengedwithinfectioushypodermalandhematopoieticnecrosisvirusihhnv AT singha geneexpressionandfunctionalstudiesofsmallheatshockprotein37mrhsp37frommacrobrachiumrosenbergiichallengedwithinfectioushypodermalandhematopoieticnecrosisvirusihhnv AT othmanry geneexpressionandfunctionalstudiesofsmallheatshockprotein37mrhsp37frommacrobrachiumrosenbergiichallengedwithinfectioushypodermalandhematopoieticnecrosisvirusihhnv AT bhassus geneexpressionandfunctionalstudiesofsmallheatshockprotein37mrhsp37frommacrobrachiumrosenbergiichallengedwithinfectioushypodermalandhematopoieticnecrosisvirusihhnv |