Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi.
Binding studies of 160 overlapping, synthetic octapeptides from the hydrophilic regions of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi with sera from patients with scrub typhus revealed 15 immunodominant peptides which are recognized by all the sera tested. Further analysis of...
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| Format: | Article |
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1993
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| _version_ | 1796944624841916416 |
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| author | Lachumanan, R. Devi, S. Cheong, Y.M. Rodda, S.J. Pang, T. |
| author_facet | Lachumanan, R. Devi, S. Cheong, Y.M. Rodda, S.J. Pang, T. |
| author_sort | Lachumanan, R. |
| collection | UM |
| description | Binding studies of 160 overlapping, synthetic octapeptides from the hydrophilic regions of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi with sera from patients with scrub typhus revealed 15 immunodominant peptides which are recognized by all the sera tested. Further analysis of the specificity of peptide binding with five of these peptides indicated that the peptides showed significantly stronger binding to scrub typhus patients' sera than they did to sera from patients with other febrile illnesses common in the region, i.e., malaria, dengue fever, typhoid fever, and leptospirosis. The main antibody class binding to these peptides appears to be immunoglobulin M, and there appears to be little correlation between reactivity with peptides and antibody titers measured by the indirect immunoperoxidase test. |
| first_indexed | 2024-03-06T05:04:33Z |
| format | Article |
| id | um.eprints-319 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:04:33Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | um.eprints-3192013-12-11T07:37:45Z http://eprints.um.edu.my/319/ Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. Lachumanan, R. Devi, S. Cheong, Y.M. Rodda, S.J. Pang, T. R Medicine (General) Binding studies of 160 overlapping, synthetic octapeptides from the hydrophilic regions of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi with sera from patients with scrub typhus revealed 15 immunodominant peptides which are recognized by all the sera tested. Further analysis of the specificity of peptide binding with five of these peptides indicated that the peptides showed significantly stronger binding to scrub typhus patients' sera than they did to sera from patients with other febrile illnesses common in the region, i.e., malaria, dengue fever, typhoid fever, and leptospirosis. The main antibody class binding to these peptides appears to be immunoglobulin M, and there appears to be little correlation between reactivity with peptides and antibody titers measured by the indirect immunoperoxidase test. 1993-10 Article PeerReviewed Lachumanan, R. and Devi, S. and Cheong, Y.M. and Rodda, S.J. and Pang, T. (1993) Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. Infection and immunity, 61 (10). pp. 4527-31. ISSN 0019-9567, DOI 7691753. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC281195/?tool=pubmed 7691753 |
| spellingShingle | R Medicine (General) Lachumanan, R. Devi, S. Cheong, Y.M. Rodda, S.J. Pang, T. Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. |
| title | Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. |
| title_full | Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. |
| title_fullStr | Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. |
| title_full_unstemmed | Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. |
| title_short | Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. |
| title_sort | epitope mapping of the sta58 major outer membrane protein of rickettsia tsutsugamushi |
| topic | R Medicine (General) |
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