Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells
This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Verlag (Germany)
2009
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/3212/1/Human_amnion_as_a_novel_cell_delivery_vehicle_for_chondrogenic_mesenchymal_stem_cells.pdf |
| _version_ | 1796944956937469952 |
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| author | Tan, S.L. Sulaiman, S. Pingguan-Murphy, Belinda Selvaratnam, L. Tai, C.C. Kamarul, Tunku |
| author_facet | Tan, S.L. Sulaiman, S. Pingguan-Murphy, Belinda Selvaratnam, L. Tai, C.C. Kamarul, Tunku |
| author_sort | Tan, S.L. |
| collection | UM |
| description | This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for 15 days. Monolayer cultures were used as control for chondrogenic differentiation and HAMs without cell seeding were used as negative control. Qualitative observations were made using scanning electron microscopy analysis and quantitative analyses were based on the sulfated glycosaminoglycans (GAG) assays performed on day 1 and day 15. Histological examination of HAM substrates before seeding revealed a smooth surface in AD substrates, while the FD substrates exhibited a porous surface. Cell attachment to AD and FD substrates on day 15 was qualitatively comparable. GAG were significantly highly expressed in cells seeded on FD HAM substrates. This study indicates that processed HAM is a potentially valuable material as a cell-carrier for MSC differentiation. |
| first_indexed | 2024-03-06T05:09:28Z |
| format | Article |
| id | um.eprints-3212 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:09:28Z |
| publishDate | 2009 |
| publisher | Springer Verlag (Germany) |
| record_format | dspace |
| spelling | um.eprints-32122020-02-10T08:33:52Z http://eprints.um.edu.my/3212/ Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells Tan, S.L. Sulaiman, S. Pingguan-Murphy, Belinda Selvaratnam, L. Tai, C.C. Kamarul, Tunku R Medicine This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for 15 days. Monolayer cultures were used as control for chondrogenic differentiation and HAMs without cell seeding were used as negative control. Qualitative observations were made using scanning electron microscopy analysis and quantitative analyses were based on the sulfated glycosaminoglycans (GAG) assays performed on day 1 and day 15. Histological examination of HAM substrates before seeding revealed a smooth surface in AD substrates, while the FD substrates exhibited a porous surface. Cell attachment to AD and FD substrates on day 15 was qualitatively comparable. GAG were significantly highly expressed in cells seeded on FD HAM substrates. This study indicates that processed HAM is a potentially valuable material as a cell-carrier for MSC differentiation. Springer Verlag (Germany) 2009 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3212/1/Human_amnion_as_a_novel_cell_delivery_vehicle_for_chondrogenic_mesenchymal_stem_cells.pdf Tan, S.L. and Sulaiman, S. and Pingguan-Murphy, Belinda and Selvaratnam, L. and Tai, C.C. and Kamarul, Tunku (2009) Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells. Cell and Tissue Banking, 12 (1). pp. 59-70. ISSN 1389-9333, DOI https://doi.org/10.1007/s10561-009-9164-x <https://doi.org/10.1007/s10561-009-9164-x>. https://doi.org/10.1007/s10561-009-9164-x doi:10.1007/s10561-009-9164-x |
| spellingShingle | R Medicine Tan, S.L. Sulaiman, S. Pingguan-Murphy, Belinda Selvaratnam, L. Tai, C.C. Kamarul, Tunku Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| title | Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| title_full | Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| title_fullStr | Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| title_full_unstemmed | Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| title_short | Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| title_sort | human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3212/1/Human_amnion_as_a_novel_cell_delivery_vehicle_for_chondrogenic_mesenchymal_stem_cells.pdf |
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