Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with di...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SEAMEO Regional Tropical Medicine and Public Health Network
2003
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| Online Access: | http://eprints.um.edu.my/3275/1/Recombinant_expression_of_Toxocara_canis_excretory-secretory_antigen_TES-120_in_Escherichia_coli..pdf |
| Summary: | The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis. |
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