Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with di...
| Asıl Yazarlar: | , , , , , |
|---|---|
| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
SEAMEO Regional Tropical Medicine and Public Health Network
2003
|
| Konular: | |
| Online Erişim: | http://eprints.um.edu.my/3275/1/Recombinant_expression_of_Toxocara_canis_excretory-secretory_antigen_TES-120_in_Escherichia_coli..pdf |
| _version_ | 1825718779021099008 |
|---|---|
| author | Fong, Mun Yik Lau, Y.L. Ithoi, Init Jamaiah, I. Anuar, A.K. Rahmah, N. |
| author_facet | Fong, Mun Yik Lau, Y.L. Ithoi, Init Jamaiah, I. Anuar, A.K. Rahmah, N. |
| author_sort | Fong, Mun Yik |
| collection | UM |
| description | The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis. |
| first_indexed | 2024-03-06T05:09:34Z |
| format | Article |
| id | um.eprints-3275 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:09:34Z |
| publishDate | 2003 |
| publisher | SEAMEO Regional Tropical Medicine and Public Health Network |
| record_format | dspace |
| spelling | um.eprints-32752021-12-30T08:29:58Z http://eprints.um.edu.my/3275/ Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli Fong, Mun Yik Lau, Y.L. Ithoi, Init Jamaiah, I. Anuar, A.K. Rahmah, N. R Medicine The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis. SEAMEO Regional Tropical Medicine and Public Health Network 2003 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3275/1/Recombinant_expression_of_Toxocara_canis_excretory-secretory_antigen_TES-120_in_Escherichia_coli..pdf Fong, Mun Yik and Lau, Y.L. and Ithoi, Init and Jamaiah, I. and Anuar, A.K. and Rahmah, N. (2003) Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli. The Southeast Asian Journal of Tropical Medicine and Public Health, 34 (4). pp. 723-726. ISSN 0125-1562, https://www.tm.mahidol.ac.th/seameo/2003_34_4/06-3120.pdf |
| spellingShingle | R Medicine Fong, Mun Yik Lau, Y.L. Ithoi, Init Jamaiah, I. Anuar, A.K. Rahmah, N. Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli |
| title | Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli |
| title_full | Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli |
| title_fullStr | Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli |
| title_full_unstemmed | Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli |
| title_short | Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli |
| title_sort | recombinant expression of toxocara canis excretory secretory antigen tes 120 in escherichia coli |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3275/1/Recombinant_expression_of_Toxocara_canis_excretory-secretory_antigen_TES-120_in_Escherichia_coli..pdf |
| work_keys_str_mv | AT fongmunyik recombinantexpressionoftoxocaracanisexcretorysecretoryantigentes120inescherichiacoli AT lauyl recombinantexpressionoftoxocaracanisexcretorysecretoryantigentes120inescherichiacoli AT ithoiinit recombinantexpressionoftoxocaracanisexcretorysecretoryantigentes120inescherichiacoli AT jamaiahi recombinantexpressionoftoxocaracanisexcretorysecretoryantigentes120inescherichiacoli AT anuarak recombinantexpressionoftoxocaracanisexcretorysecretoryantigentes120inescherichiacoli AT rahmahn recombinantexpressionoftoxocaracanisexcretorysecretoryantigentes120inescherichiacoli |