Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay
There are several methods for the detection of haemolytic activity in campylobacters. However, we found the haemolytic effect of campylobacters on conventional blood agar plates to be variable, inconsistent and difficult to interpret. Blood agarose plates showed campylobacter haemolytic activity mor...
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| 格式: | 文件 |
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Microbiology Society
1995
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| _version_ | 1825718434972827648 |
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| author | Tay, S.T. Devi, S. Puthucheary, S.D. Kautner, I.M. |
| author_facet | Tay, S.T. Devi, S. Puthucheary, S.D. Kautner, I.M. |
| author_sort | Tay, S.T. |
| collection | UM |
| description | There are several methods for the detection of haemolytic activity in campylobacters. However, we found the haemolytic effect of campylobacters on conventional blood agar plates to be variable, inconsistent and difficult to interpret. Blood agarose plates showed campylobacter haemolytic activity more clearly. The incubation conditions (temperature and gaseous) appear to be important for the expression of this activity. Ninety four percent of the Campylobacter isolates examined were found to be haemolytic by the microplate assay with minimal haemolytic units that ranged from 1 to 64. Haemolytic activity was detected only from live bacterial cultures and not from any of the 50 bacterial culture supernates, which suggests that campylobacters may possess a cell-associated haemolysin. The identification of such haemolytic activity in a large number of campylobacters (94%) suggests its potential role as a virulence factor in campylobacter gastroenteritis. |
| first_indexed | 2024-03-06T05:04:34Z |
| format | Article |
| id | um.eprints-334 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:04:34Z |
| publishDate | 1995 |
| publisher | Microbiology Society |
| record_format | dspace |
| spelling | um.eprints-3342019-04-18T07:56:39Z http://eprints.um.edu.my/334/ Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay Tay, S.T. Devi, S. Puthucheary, S.D. Kautner, I.M. R Medicine (General) There are several methods for the detection of haemolytic activity in campylobacters. However, we found the haemolytic effect of campylobacters on conventional blood agar plates to be variable, inconsistent and difficult to interpret. Blood agarose plates showed campylobacter haemolytic activity more clearly. The incubation conditions (temperature and gaseous) appear to be important for the expression of this activity. Ninety four percent of the Campylobacter isolates examined were found to be haemolytic by the microplate assay with minimal haemolytic units that ranged from 1 to 64. Haemolytic activity was detected only from live bacterial cultures and not from any of the 50 bacterial culture supernates, which suggests that campylobacters may possess a cell-associated haemolysin. The identification of such haemolytic activity in a large number of campylobacters (94%) suggests its potential role as a virulence factor in campylobacter gastroenteritis. Microbiology Society 1995-03 Article PeerReviewed Tay, S.T. and Devi, S. and Puthucheary, S.D. and Kautner, I.M. (1995) Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay. Journal of Medical Microbiology, 42 (3). pp. 175-80. ISSN 0022-2615, DOI 7884798. http://www.ncbi.nlm.nih.gov/pubmed/7884798 7884798 |
| spellingShingle | R Medicine (General) Tay, S.T. Devi, S. Puthucheary, S.D. Kautner, I.M. Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| title | Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| title_full | Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| title_fullStr | Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| title_full_unstemmed | Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| title_short | Detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| title_sort | detection of haemolytic activity of campylobacters by agarose haemolysis and microplate assay |
| topic | R Medicine (General) |
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