Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre
Gymnema sylvestre (R.Br.) is an important diabetic medicinal plant which yields pharmaceutically active compounds called gymnemic acid (GA). The present study describes callus induction and the subsequent batch culture optimization and GA quantification determined by linearity, precision, accuracy,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2012
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/3391/1/14.pdf |
| _version_ | 1825718795134566400 |
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| author | Ahmed, A.B. Rao, A.S. Rao, M.V. Taha, R.M. |
| author_facet | Ahmed, A.B. Rao, A.S. Rao, M.V. Taha, R.M. |
| author_sort | Ahmed, A.B. |
| collection | UM |
| description | Gymnema sylvestre (R.Br.) is an important diabetic medicinal plant which yields pharmaceutically active compounds called gymnemic acid (GA). The present study describes callus induction and the subsequent batch culture optimization and GA quantification determined by linearity, precision, accuracy, and recovery. Best callus induction of GA was noticed in MS medium combined with 2,4-D (1.5 mg/L) and KN (0.5 mg/L). Evaluation and isolation of GA from the calluses derived from different plant parts, namely, leaf, stem and petioles have been done in the present case for the first time. Factors such as light, temperature, sucrose, and photoperiod were studied to observe their effect on GA production. Temperature conditions completely inhibited GA production. Out of the different sucrose concentrations tested, the highest yield (35.4 mg/g d.w) was found at 5% sucrose followed by 12 h photoperiod (26.86 mg/g d.w). Maximum GA production (58.28 mg/g d.w) was observed in blue light. The results showed that physical and chemical factors greatly influence the production of GA in callus cultures of G. sylvestre. The factors optimized for in vitro production of GA during the present study can successfully be employed for their large-scale production in bioreactors. |
| first_indexed | 2024-03-06T05:09:48Z |
| format | Article |
| id | um.eprints-3391 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:09:48Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | um.eprints-33912017-07-06T00:59:22Z http://eprints.um.edu.my/3391/ Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre Ahmed, A.B. Rao, A.S. Rao, M.V. Taha, R.M. R Medicine Gymnema sylvestre (R.Br.) is an important diabetic medicinal plant which yields pharmaceutically active compounds called gymnemic acid (GA). The present study describes callus induction and the subsequent batch culture optimization and GA quantification determined by linearity, precision, accuracy, and recovery. Best callus induction of GA was noticed in MS medium combined with 2,4-D (1.5 mg/L) and KN (0.5 mg/L). Evaluation and isolation of GA from the calluses derived from different plant parts, namely, leaf, stem and petioles have been done in the present case for the first time. Factors such as light, temperature, sucrose, and photoperiod were studied to observe their effect on GA production. Temperature conditions completely inhibited GA production. Out of the different sucrose concentrations tested, the highest yield (35.4 mg/g d.w) was found at 5% sucrose followed by 12 h photoperiod (26.86 mg/g d.w). Maximum GA production (58.28 mg/g d.w) was observed in blue light. The results showed that physical and chemical factors greatly influence the production of GA in callus cultures of G. sylvestre. The factors optimized for in vitro production of GA during the present study can successfully be employed for their large-scale production in bioreactors. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3391/1/14.pdf Ahmed, A.B. and Rao, A.S. and Rao, M.V. and Taha, R.M. (2012) Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre. Scientific World Journal. ISSN 2356-6140, DOI PMID: 22629221. http://www.ncbi.nlm.nih.gov/pubmed/22629221 PMID: 22629221 |
| spellingShingle | R Medicine Ahmed, A.B. Rao, A.S. Rao, M.V. Taha, R.M. Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre |
| title | Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre |
| title_full | Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre |
| title_fullStr | Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre |
| title_full_unstemmed | Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre |
| title_short | Production of gymnemic acid depends on medium, explants, pgrs, color lights, temperature, photoperiod, and sucrose sources in batch culture of gymnema sylvestre |
| title_sort | production of gymnemic acid depends on medium explants pgrs color lights temperature photoperiod and sucrose sources in batch culture of gymnema sylvestre |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3391/1/14.pdf |
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