The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins
Champedak (Artocarpus integer) lectin-M is a lectin with high specificity and affinity for the core-mannosyl residues of the N-linked oligosaccharides of glycoproteins. We have studied the interaction of the champedak seed lectin with human serum glycoproteins that were resolved by 2-dimensional (2-...
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| Format: | Article |
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2001
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| author | Hashim, Onn Haji Ahmad, F. Shuib, A.S. |
| author_facet | Hashim, Onn Haji Ahmad, F. Shuib, A.S. |
| author_sort | Hashim, Onn Haji |
| collection | UM |
| description | Champedak (Artocarpus integer) lectin-M is a lectin with high specificity and affinity for the core-mannosyl residues of the N-linked oligosaccharides of glycoproteins. We have studied the interaction of the champedak seed lectin with human serum glycoproteins that were resolved by 2-dimensional (2-D) gel electrophoresis. The lectin demonstrated strong interaction with haptoglobin beta chain, orosomucoid, al-antitrypsin, alpha (2)-HS glycoprotein, transferrin, hemopexin, alpha B-1-glycoprotein, and the heavy chains of IgA, IgM and IgG of the human serum. With exceptions of the heavy chains of the immunoglobulins and alpha B-1-glycoprotein, all the other lectin-M-probed glycopeptides are acute-phase proteins. The use of champedak lectin-h l to probe for serum glycoproteins that were separated in a 2-D gel electrophoresis and Western blotting technique may be conveniently applied to analyse the acute-phase and humoral immune responses simultaneously. Subjecting human serum to immobilised-lectin-M affinity chromatography was able to isolate intact haptoglobin, alpha (1)-antitrypsin, alpha B-1-glycoprotein, hemopexin and IgA. |
| first_indexed | 2024-03-06T05:09:56Z |
| format | Article |
| id | um.eprints-3445 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:09:56Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | um.eprints-34452019-10-24T08:20:32Z http://eprints.um.edu.my/3445/ The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins Hashim, Onn Haji Ahmad, F. Shuib, A.S. R Medicine Champedak (Artocarpus integer) lectin-M is a lectin with high specificity and affinity for the core-mannosyl residues of the N-linked oligosaccharides of glycoproteins. We have studied the interaction of the champedak seed lectin with human serum glycoproteins that were resolved by 2-dimensional (2-D) gel electrophoresis. The lectin demonstrated strong interaction with haptoglobin beta chain, orosomucoid, al-antitrypsin, alpha (2)-HS glycoprotein, transferrin, hemopexin, alpha B-1-glycoprotein, and the heavy chains of IgA, IgM and IgG of the human serum. With exceptions of the heavy chains of the immunoglobulins and alpha B-1-glycoprotein, all the other lectin-M-probed glycopeptides are acute-phase proteins. The use of champedak lectin-h l to probe for serum glycoproteins that were separated in a 2-D gel electrophoresis and Western blotting technique may be conveniently applied to analyse the acute-phase and humoral immune responses simultaneously. Subjecting human serum to immobilised-lectin-M affinity chromatography was able to isolate intact haptoglobin, alpha (1)-antitrypsin, alpha B-1-glycoprotein, hemopexin and IgA. 2001 Article PeerReviewed Hashim, Onn Haji and Ahmad, F. and Shuib, A.S. (2001) The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins. Immunological Investigations, 30 (2). pp. 131-141. ISSN 0882-0139, DOI https://doi.org/10.1081/imm-100104021 <https://doi.org/10.1081/imm-100104021>. http://informahealthcare.com/doi/abs/10.1081/IMM-100104021 10.1081/imm-100104021 |
| spellingShingle | R Medicine Hashim, Onn Haji Ahmad, F. Shuib, A.S. The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins |
| title | The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins |
| title_full | The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins |
| title_fullStr | The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins |
| title_full_unstemmed | The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins |
| title_short | The application of Artocarpus integer seed lectin-M in the detection and isolation of selective human serum acute-phase proteins and immunoglobulins |
| title_sort | application of artocarpus integer seed lectin m in the detection and isolation of selective human serum acute phase proteins and immunoglobulins |
| topic | R Medicine |
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