Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l
A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of appro...
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Format: | Article |
Language: | English |
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Academic Journals
2009
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Online Access: | http://eprints.um.edu.my/3789/1/Cloning_and_Expression_of_a_Vi_Mimotope_of_Salmonella_enterica_serovar_Typhi_through_Nucleotide-Nucleotide_Hybridization_Approach..pdf |
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author | Chua, Kek Heng Kee, B. Thong, Kwai Lin |
author_facet | Chua, Kek Heng Kee, B. Thong, Kwai Lin |
author_sort | Chua, Kek Heng |
collection | UM |
description | A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual. |
first_indexed | 2024-03-06T05:10:43Z |
format | Article |
id | um.eprints-3789 |
institution | Universiti Malaya |
language | English |
last_indexed | 2024-03-06T05:10:43Z |
publishDate | 2009 |
publisher | Academic Journals |
record_format | dspace |
spelling | um.eprints-37892018-10-15T03:26:39Z http://eprints.um.edu.my/3789/ Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l Chua, Kek Heng Kee, B. Thong, Kwai Lin R Medicine A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual. Academic Journals 2009 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3789/1/Cloning_and_Expression_of_a_Vi_Mimotope_of_Salmonella_enterica_serovar_Typhi_through_Nucleotide-Nucleotide_Hybridization_Approach..pdf Chua, Kek Heng and Kee, B. and Thong, Kwai Lin (2009) Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l. African Journal of Biotechnology, 8 (18). pp. 4623-4626. ISSN 1684-5315, https://www.ajol.info/index.php/ajb/article/view/62427 |
spellingShingle | R Medicine Chua, Kek Heng Kee, B. Thong, Kwai Lin Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l |
title | Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l |
title_full | Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l |
title_fullStr | Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l |
title_full_unstemmed | Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l |
title_short | Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l |
title_sort | cloning and expression of a vi mimotope of salmonella enterica serovar typhi through nucleotide nucleotide hybridization approach l |
topic | R Medicine |
url | http://eprints.um.edu.my/3789/1/Cloning_and_Expression_of_a_Vi_Mimotope_of_Salmonella_enterica_serovar_Typhi_through_Nucleotide-Nucleotide_Hybridization_Approach..pdf |
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