Isolate resistance of Blastocystis hominis to metronidazole
Isolates of Blastocystis hominis from infected immigrant workers from Indonesia, Bangladesh and infected individuals from Singapore and Malaysia were assessed for growth pattern and degree of resistance to different concentrations of metronidazole. Viability of the cells was assessed using eosin-bri...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Wiley
1999
|
| Subjects: |
| _version_ | 1825718439554056192 |
|---|---|
| author | Haresh, K. Suresh, K. Khairul Anus, A. Saminathan, S. |
| author_facet | Haresh, K. Suresh, K. Khairul Anus, A. Saminathan, S. |
| author_sort | Haresh, K. |
| collection | UM |
| description | Isolates of Blastocystis hominis from infected immigrant workers from Indonesia, Bangladesh and infected individuals from Singapore and Malaysia were assessed for growth pattern and degree of resistance to different concentrations of metronidazole. Viability of the cells was assessed using eosin-brillian cresyl blue which stained viable cells green and nonviable cells red. The Bangladeshi and Singaporean isolates were nonviable even at the lowest concentration of 0.01 mg/ml, whereas 40% of the initial inoculum of parasites from the Indonesian isolate at day one were still viable in cultures with 1.0 mg/ml metronidazole. The study shows that isolates of B. hominis of different geographical origin have different levels of resistance to metronidazole. The search for more effective drugs to eliminate th parasite appears inevitable, especially since surviving parasites from metronidazole cultures show greater ability to multiply in subcultures than controls. |
| first_indexed | 2024-03-06T05:04:38Z |
| format | Article |
| id | um.eprints-386 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:04:38Z |
| publishDate | 1999 |
| publisher | Wiley |
| record_format | dspace |
| spelling | um.eprints-3862019-02-13T08:55:33Z http://eprints.um.edu.my/386/ Isolate resistance of Blastocystis hominis to metronidazole Haresh, K. Suresh, K. Khairul Anus, A. Saminathan, S. R Medicine (General) Isolates of Blastocystis hominis from infected immigrant workers from Indonesia, Bangladesh and infected individuals from Singapore and Malaysia were assessed for growth pattern and degree of resistance to different concentrations of metronidazole. Viability of the cells was assessed using eosin-brillian cresyl blue which stained viable cells green and nonviable cells red. The Bangladeshi and Singaporean isolates were nonviable even at the lowest concentration of 0.01 mg/ml, whereas 40% of the initial inoculum of parasites from the Indonesian isolate at day one were still viable in cultures with 1.0 mg/ml metronidazole. The study shows that isolates of B. hominis of different geographical origin have different levels of resistance to metronidazole. The search for more effective drugs to eliminate th parasite appears inevitable, especially since surviving parasites from metronidazole cultures show greater ability to multiply in subcultures than controls. Wiley 1999-04 Article PeerReviewed Haresh, K. and Suresh, K. and Khairul Anus, A. and Saminathan, S. (1999) Isolate resistance of Blastocystis hominis to metronidazole. Tropical Medicine and International Health, 4 (4). pp. 274-7. ISSN 1360-2276, DOI 10357863. http://www.ncbi.nlm.nih.gov/pubmed/10357863 10357863 |
| spellingShingle | R Medicine (General) Haresh, K. Suresh, K. Khairul Anus, A. Saminathan, S. Isolate resistance of Blastocystis hominis to metronidazole |
| title | Isolate resistance of Blastocystis hominis to metronidazole |
| title_full | Isolate resistance of Blastocystis hominis to metronidazole |
| title_fullStr | Isolate resistance of Blastocystis hominis to metronidazole |
| title_full_unstemmed | Isolate resistance of Blastocystis hominis to metronidazole |
| title_short | Isolate resistance of Blastocystis hominis to metronidazole |
| title_sort | isolate resistance of blastocystis hominis to metronidazole |
| topic | R Medicine (General) |
| work_keys_str_mv | AT hareshk isolateresistanceofblastocystishoministometronidazole AT sureshk isolateresistanceofblastocystishoministometronidazole AT khairulanusa isolateresistanceofblastocystishoministometronidazole AT saminathans isolateresistanceofblastocystishoministometronidazole |