Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry
Binding of dengue virus 2 (DENV-2) to C6/36 mosquito cells protein was investigated. A 48 kDa DENV-2-binding C6/36 cells protein (D2BP) was detected in a virus overlay protein-binding assay. The binding occurred only to the C6/36 cells cytosolic protein fraction and it was inhibited by free D2BP. D2...
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| Format: | Article |
| Language: | English |
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2004
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| Online Access: | http://eprints.um.edu.my/3923/1/Chee-2004-Identification_of_a.pdf |
| _version_ | 1796945058449063936 |
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| author | Chee, H.Y. AbuBakar, Sazaly |
| author_facet | Chee, H.Y. AbuBakar, Sazaly |
| author_sort | Chee, H.Y. |
| collection | UM |
| description | Binding of dengue virus 2 (DENV-2) to C6/36 mosquito cells protein was investigated. A 48 kDa DENV-2-binding C6/36 cells protein (D2BP) was detected in a virus overlay protein-binding assay. The binding occurred only to the C6/36 cells cytosolic protein fraction and it was inhibited by free D2BP. D2BP was shown to bind to DENV-2 E in the far-Western-binding studies and using mass spectrometry (MS) and MS/MS, peptide masses of the D2BP that matched to beta-tubulin and alpha-tubulin chains were identified. These findings suggest that DENV-2 through DENV-2 E binds directly to a 48 kDa tubulin or tubulin-like protein of C6/36 mosquito cells. (C) 2004 Elsevier Inc. All rights reserved. |
| first_indexed | 2024-03-06T05:10:59Z |
| format | Article |
| id | um.eprints-3923 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:10:59Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | um.eprints-39232019-02-13T08:08:50Z http://eprints.um.edu.my/3923/ Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry Chee, H.Y. AbuBakar, Sazaly R Medicine Binding of dengue virus 2 (DENV-2) to C6/36 mosquito cells protein was investigated. A 48 kDa DENV-2-binding C6/36 cells protein (D2BP) was detected in a virus overlay protein-binding assay. The binding occurred only to the C6/36 cells cytosolic protein fraction and it was inhibited by free D2BP. D2BP was shown to bind to DENV-2 E in the far-Western-binding studies and using mass spectrometry (MS) and MS/MS, peptide masses of the D2BP that matched to beta-tubulin and alpha-tubulin chains were identified. These findings suggest that DENV-2 through DENV-2 E binds directly to a 48 kDa tubulin or tubulin-like protein of C6/36 mosquito cells. (C) 2004 Elsevier Inc. All rights reserved. 2004 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3923/1/Chee-2004-Identification_of_a.pdf Chee, H.Y. and AbuBakar, Sazaly (2004) Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry. Biochemical and Biophysical Research Communications, 320 (1). pp. 11-17. ISSN 0006-291X, DOI 15207695. http://www.ncbi.nlm.nih.gov/pubmed/15207695 15207695 |
| spellingShingle | R Medicine Chee, H.Y. AbuBakar, Sazaly Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| title | Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| title_full | Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| title_fullStr | Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| title_full_unstemmed | Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| title_short | Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| title_sort | identification of a 48 kda tubulin or tubulin like c6 36 mosquito cells protein that binds dengue virus 2 using mass spectrometry |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3923/1/Chee-2004-Identification_of_a.pdf |
| work_keys_str_mv | AT cheehy identificationofa48kdatubulinortubulinlikec636mosquitocellsproteinthatbindsdenguevirus2usingmassspectrometry AT abubakarsazaly identificationofa48kdatubulinortubulinlikec636mosquitocellsproteinthatbindsdenguevirus2usingmassspectrometry |