In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2

In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polyme...

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প্রধান লেখক: Zandi, K., Teoh, B.T., Sam, S.S., Wong, P.F., Mustafa, Mohd Rais, AbuBakar, Sazaly
বিন্যাস: প্রবন্ধ
প্রকাশিত: 2011
বিষয়গুলি:
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author Zandi, K.
Teoh, B.T.
Sam, S.S.
Wong, P.F.
Mustafa, Mohd Rais
AbuBakar, Sazaly
author_facet Zandi, K.
Teoh, B.T.
Sam, S.S.
Wong, P.F.
Mustafa, Mohd Rais
AbuBakar, Sazaly
author_sort Zandi, K.
collection UM
description In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50 cytotoxicity (CC(50)) values of 247, > 1000, and 87 mu g/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC(50)) value of 55 mu g/mL and selectivity index (SI) of 4.49. The IC(50) value of fisetin was 43.12 mu g/mL with SI=5.72 when Vero cells were treated for 5 h before virus infection and continuously up to 4 days post-infection. There was no direct virucidal activity or prophylactic activity of fisetin against DENV-2. Rutin and naringenin did not inhibit DENV-2 replication in Vero cells. Naringenin however, exhibited direct virucidal activity against DENV-2 with IC(50) = 52.64 mu g/mL but the SI was <1. The present study suggests that among the flavonoids examined, only fisetin showed significant in vitro anti dengue virus replication activity.
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spelling um.eprints-39632019-12-18T06:33:53Z http://eprints.um.edu.my/3963/ In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2 Zandi, K. Teoh, B.T. Sam, S.S. Wong, P.F. Mustafa, Mohd Rais AbuBakar, Sazaly R Medicine In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50 cytotoxicity (CC(50)) values of 247, > 1000, and 87 mu g/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC(50)) value of 55 mu g/mL and selectivity index (SI) of 4.49. The IC(50) value of fisetin was 43.12 mu g/mL with SI=5.72 when Vero cells were treated for 5 h before virus infection and continuously up to 4 days post-infection. There was no direct virucidal activity or prophylactic activity of fisetin against DENV-2. Rutin and naringenin did not inhibit DENV-2 replication in Vero cells. Naringenin however, exhibited direct virucidal activity against DENV-2 with IC(50) = 52.64 mu g/mL but the SI was <1. The present study suggests that among the flavonoids examined, only fisetin showed significant in vitro anti dengue virus replication activity. 2011 Article PeerReviewed Zandi, K. and Teoh, B.T. and Sam, S.S. and Wong, P.F. and Mustafa, Mohd Rais and AbuBakar, Sazaly (2011) In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2. Journal of Medicinal Plants Research, 5 (23). pp. 5534-5539. ISSN 1996-0875,
spellingShingle R Medicine
Zandi, K.
Teoh, B.T.
Sam, S.S.
Wong, P.F.
Mustafa, Mohd Rais
AbuBakar, Sazaly
In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2
title In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2
title_full In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2
title_fullStr In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2
title_full_unstemmed In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2
title_short In vitro antiviral activity of Fisetin, Rutin and Naringenin against Dengue virus type-2
title_sort in vitro antiviral activity of fisetin rutin and naringenin against dengue virus type 2
topic R Medicine
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