High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells
Malignant prostate tissues have markedly reduced zinc (Zn(2+)) contents in comparison to non-malignant tissues. In this study, we restored a high intracellular Zn(2+) level to LNCaP prostate cancer cells by culturing the cells in a growth medium supplemented with a supraphysiological concentration o...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Published: |
2008
|
| Subjects: |
| _version_ | 1825718878565564416 |
|---|---|
| author | Wong, P.F. AbuBakar, Sazaly |
| author_facet | Wong, P.F. AbuBakar, Sazaly |
| author_sort | Wong, P.F. |
| collection | UM |
| description | Malignant prostate tissues have markedly reduced zinc (Zn(2+)) contents in comparison to non-malignant tissues. In this study, we restored a high intracellular Zn(2+) level to LNCaP prostate cancer cells by culturing the cells in a growth medium supplemented with a supraphysiological concentration of Zn(2+) (10 mu g/ml) over 5 weeks. The intracellular Zn(2+) level increased in the Zn(2+)-treated cells, and there was a marked increase in the presence of zincosomes, a Zn(2+)-specific intracellular organelle. The proliferation rate of the Zn(2+)-treated cells was markedly reduced. There was also a significant increase (36.6 +/- 6.4) in the total tyrosine phosphorylated proteins. Vaccinia H1-related (VHR) phosphatase, zeta chain-associated protein-70 (ZAP-70) kinase and phosphorylated extracellular signal-regulated protein kinase 1 and 2 (p-ERK 1 and 2) were also present in higher abundance. Treatment with TPEN, which chelates Zn(2+), reduced the abundance of VHR phosphatase and ZAP-70 kinase, but increased the abundance of p-ERK 1. However, the TPEN treatment restored the Zn(2+)-treated LNCaP cell proliferation to a rate comparable to that of the non Zn(2+)-treated cells. These results highlight the importance of a high intracellular Zn(2+) content and the VHR/ZAP-70-associated pathways in the modulation of LNCaP prostate cancer cell growth. |
| first_indexed | 2024-03-06T05:11:03Z |
| format | Article |
| id | um.eprints-3969 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:11:03Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | um.eprints-39692019-02-13T08:10:46Z http://eprints.um.edu.my/3969/ High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells Wong, P.F. AbuBakar, Sazaly R Medicine Malignant prostate tissues have markedly reduced zinc (Zn(2+)) contents in comparison to non-malignant tissues. In this study, we restored a high intracellular Zn(2+) level to LNCaP prostate cancer cells by culturing the cells in a growth medium supplemented with a supraphysiological concentration of Zn(2+) (10 mu g/ml) over 5 weeks. The intracellular Zn(2+) level increased in the Zn(2+)-treated cells, and there was a marked increase in the presence of zincosomes, a Zn(2+)-specific intracellular organelle. The proliferation rate of the Zn(2+)-treated cells was markedly reduced. There was also a significant increase (36.6 +/- 6.4) in the total tyrosine phosphorylated proteins. Vaccinia H1-related (VHR) phosphatase, zeta chain-associated protein-70 (ZAP-70) kinase and phosphorylated extracellular signal-regulated protein kinase 1 and 2 (p-ERK 1 and 2) were also present in higher abundance. Treatment with TPEN, which chelates Zn(2+), reduced the abundance of VHR phosphatase and ZAP-70 kinase, but increased the abundance of p-ERK 1. However, the TPEN treatment restored the Zn(2+)-treated LNCaP cell proliferation to a rate comparable to that of the non Zn(2+)-treated cells. These results highlight the importance of a high intracellular Zn(2+) content and the VHR/ZAP-70-associated pathways in the modulation of LNCaP prostate cancer cell growth. 2008 Article PeerReviewed Wong, P.F. and AbuBakar, Sazaly (2008) High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells. Cellular & Molecular Biology Letters, 13 (3). pp. 375-390. ISSN 1689-1392, DOI 18311544. http://www.ncbi.nlm.nih.gov/pubmed/18311544 18311544 |
| spellingShingle | R Medicine Wong, P.F. AbuBakar, Sazaly High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells |
| title | High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells |
| title_full | High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells |
| title_fullStr | High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells |
| title_full_unstemmed | High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells |
| title_short | High intracellular Zn(2+) ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells |
| title_sort | high intracellular zn 2 ions modulate the vhr zap 70 and erk activities of lncap prostate cancer cells |
| topic | R Medicine |
| work_keys_str_mv | AT wongpf highintracellularzn2ionsmodulatethevhrzap70anderkactivitiesoflncapprostatecancercells AT abubakarsazaly highintracellularzn2ionsmodulatethevhrzap70anderkactivitiesoflncapprostatecancercells |