Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice
Dengue virus Type 2 C, prM, truncated E (NB-E and B-E), NS1, NS2A, NS2B/3 and NS3 genes were cloned and expressed using the pEGFP-N1 eukaryotic expression vector. Protein expression was visualized in transfected Vero cell cultures. Immunogenicity of the recombinant DNA plasmids was determined by in...
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| Format: | Article |
| Language: | English |
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2006
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| Online Access: | http://eprints.um.edu.my/3986/1/14-20.pdf |
| _version_ | 1796945064585330688 |
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| author | Shafee, N. AbuBakar, Sazaly |
| author_facet | Shafee, N. AbuBakar, Sazaly |
| author_sort | Shafee, N. |
| collection | UM |
| description | Dengue virus Type 2 C, prM, truncated E (NB-E and B-E), NS1, NS2A, NS2B/3 and NS3 genes were cloned and expressed using the pEGFP-N1 eukaryotic expression vector. Protein expression was visualized in transfected Vero cell cultures. Immunogenicity of the recombinant DNA plasmids was determined by inoculating 8 weeks old BALB/cJ mice with two doses of plasmid cocktail followed by a booster inoculation at two weeks apart. Mice were also injected with empty vector plasmids or vaccinated with virus inoculum as controls. Initial inoculation with the plasmid cocktail resulted in IgG response comparable to inoculation with virus inoculum alone which peaked after the booster dose. Rapid rise in IgG response was also observed when the mice were subsequently challenged with partially purified dengue virus Type 2, ten weeks after the first inoculation. These results suggest for the first time, that dengue virus DNA genes expressing antigens as fusion proteins with EGFP could stimulate immune responses in vivo. |
| first_indexed | 2024-03-06T05:11:04Z |
| format | Article |
| id | um.eprints-3986 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:11:04Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | um.eprints-39862019-02-13T08:05:21Z http://eprints.um.edu.my/3986/ Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice Shafee, N. AbuBakar, Sazaly R Medicine Dengue virus Type 2 C, prM, truncated E (NB-E and B-E), NS1, NS2A, NS2B/3 and NS3 genes were cloned and expressed using the pEGFP-N1 eukaryotic expression vector. Protein expression was visualized in transfected Vero cell cultures. Immunogenicity of the recombinant DNA plasmids was determined by inoculating 8 weeks old BALB/cJ mice with two doses of plasmid cocktail followed by a booster inoculation at two weeks apart. Mice were also injected with empty vector plasmids or vaccinated with virus inoculum as controls. Initial inoculation with the plasmid cocktail resulted in IgG response comparable to inoculation with virus inoculum alone which peaked after the booster dose. Rapid rise in IgG response was also observed when the mice were subsequently challenged with partially purified dengue virus Type 2, ten weeks after the first inoculation. These results suggest for the first time, that dengue virus DNA genes expressing antigens as fusion proteins with EGFP could stimulate immune responses in vivo. 2006 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3986/1/14-20.pdf Shafee, N. and AbuBakar, Sazaly (2006) Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice. International Journal of Virology, 2 (1). pp. 14-20. DOI https://doi.org/10.3923/ijv.2006.14.20 <https://doi.org/10.3923/ijv.2006.14.20>. http://scialert.net/abstract/?doi=ijv.2006.14.20 10.3923/ijv.2006.14.20 |
| spellingShingle | R Medicine Shafee, N. AbuBakar, Sazaly Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice |
| title | Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice |
| title_full | Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice |
| title_fullStr | Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice |
| title_full_unstemmed | Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice |
| title_short | Immunization with DNA vectors consisting of selected Dengue 2 virus genes stimulated antibody Responses in Mice |
| title_sort | immunization with dna vectors consisting of selected dengue 2 virus genes stimulated antibody responses in mice |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3986/1/14-20.pdf |
| work_keys_str_mv | AT shafeen immunizationwithdnavectorsconsistingofselecteddengue2virusgenesstimulatedantibodyresponsesinmice AT abubakarsazaly immunizationwithdnavectorsconsistingofselecteddengue2virusgenesstimulatedantibodyresponsesinmice |