Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta)
The application of proteomics in alga research is still quite limited. The present report describes the establishment of the proteome of a red alga of economic importance, Gracilaria changii (Xia et Abbott) Abbott, Zhang et Xia. Initially, four protein extraction methods including direct precipitati...
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| 格式: | 文件 |
| 语言: | English |
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2006
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| 在线阅读: | http://eprints.um.edu.my/3998/1/Wong-2006-Proteomics_of_the_re.pdf |
| _version_ | 1825718880711999488 |
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| author | Wong, P.F. Tan, L.J. Nawi, H. AbuBakar, Sazaly |
| author_facet | Wong, P.F. Tan, L.J. Nawi, H. AbuBakar, Sazaly |
| author_sort | Wong, P.F. |
| collection | UM |
| description | The application of proteomics in alga research is still quite limited. The present report describes the establishment of the proteome of a red alga of economic importance, Gracilaria changii (Xia et Abbott) Abbott, Zhang et Xia. Initially, four protein extraction methods including direct precipitation by trichloroacetic acid/acetone, direct lysis using urea buffer, Tris buffer, and phenol/chloroform extraction were compared for their suitability to generate G. changii proteins for two-dimensional gel electrophoresis (2-DE). The phenol/chloroform protein extraction method gave the best 2-DE resolution of the proteins. Using these 2-DE gels and mass spectrometry, several proteins including pigment proteins, metabolic enzymes, and ion transporters were identified. These findings highlight the potential of using proteomic approaches for the investigation of G. changii protein function. |
| first_indexed | 2024-03-06T05:11:05Z |
| format | Article |
| id | um.eprints-3998 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:11:05Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | um.eprints-39982019-02-13T08:06:07Z http://eprints.um.edu.my/3998/ Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) Wong, P.F. Tan, L.J. Nawi, H. AbuBakar, Sazaly R Medicine The application of proteomics in alga research is still quite limited. The present report describes the establishment of the proteome of a red alga of economic importance, Gracilaria changii (Xia et Abbott) Abbott, Zhang et Xia. Initially, four protein extraction methods including direct precipitation by trichloroacetic acid/acetone, direct lysis using urea buffer, Tris buffer, and phenol/chloroform extraction were compared for their suitability to generate G. changii proteins for two-dimensional gel electrophoresis (2-DE). The phenol/chloroform protein extraction method gave the best 2-DE resolution of the proteins. Using these 2-DE gels and mass spectrometry, several proteins including pigment proteins, metabolic enzymes, and ion transporters were identified. These findings highlight the potential of using proteomic approaches for the investigation of G. changii protein function. 2006 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3998/1/Wong-2006-Proteomics_of_the_re.pdf Wong, P.F. and Tan, L.J. and Nawi, H. and AbuBakar, Sazaly (2006) Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta). Journal of Phycology, 42 (1). pp. 113-120. ISSN 0022-3646, DOI https://doi.org/10.1111/j.1529-8817.2006.00182.x <https://doi.org/10.1111/j.1529-8817.2006.00182.x>. http://onlinelibrary.wiley.com/store/10.1111/j.1529-8817.2006.00182.x/asset/j.1529-8817.2006.00182.x.pdf?v=1&t=h846d1hb&s=8b8ca112ec82f9f9863c0aca75b3a183aec3bcb 10.1111/j.1529-8817.2006.00182.x |
| spellingShingle | R Medicine Wong, P.F. Tan, L.J. Nawi, H. AbuBakar, Sazaly Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) |
| title | Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) |
| title_full | Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) |
| title_fullStr | Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) |
| title_full_unstemmed | Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) |
| title_short | Proteomics of the red alga, Gracilaria changii (Gracilariales, Rhodophyta) |
| title_sort | proteomics of the red alga gracilaria changii gracilariales rhodophyta |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3998/1/Wong-2006-Proteomics_of_the_re.pdf |
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