Enterovirus 71 Infection Induces Apoptosis in Vero Cells
The effects of Enterovirus 71 (HEV71) infection on African green monkey kidney cells (Vero) were investigated. It was found that the infected cells showed progressive cellular morphological changes characteristic in apoptotic cells within 10 hours post-infection. The number of apoptotic cells correl...
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| Format: | Article |
| Language: | English |
| Published: |
2003
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| Online Access: | http://eprints.um.edu.my/3999/1/Enterovirus_71_infection_induces_apoptosis_in_Vero_cells.pdf |
| _version_ | 1796945065785950208 |
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| author | Chan, Y.F. AbuBakar, Sazaly |
| author_facet | Chan, Y.F. AbuBakar, Sazaly |
| author_sort | Chan, Y.F. |
| collection | UM |
| description | The effects of Enterovirus 71 (HEV71) infection on African green monkey kidney cells (Vero) were investigated. It was found that the infected cells showed progressive cellular morphological changes characteristic in apoptotic cells within 10 hours post-infection. The number of apoptotic cells correlated significantly with the number of HEV71 antigen positive cells when cells were labeled using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and stained for HEV71 antigen. Approximately 11, 26, 45 and 50 of the infected cells were apoptotic at 12, 24, 48 and 72 hours post-infection, respectively. Internucleosomal DNA fragmentation, characteristic in the late stage of apoptosis was noted beginning on day 2 post-infection. The DNA fragmentation, however, was absent in cells treated with the heat- and ultraviolet light-inactivated virus inocula. These results demonstrate the capacity of HEV71 to induce apoptosis in the infected cells. The induction, however, requires high level of HEV71 infectivity and the presence of live virus particles, suggesting the need for the presence of specific viral proteins for apoptosis to occur. |
| first_indexed | 2024-03-06T05:11:06Z |
| format | Article |
| id | um.eprints-3999 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:11:06Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | um.eprints-39992019-02-13T08:02:12Z http://eprints.um.edu.my/3999/ Enterovirus 71 Infection Induces Apoptosis in Vero Cells Chan, Y.F. AbuBakar, Sazaly R Medicine The effects of Enterovirus 71 (HEV71) infection on African green monkey kidney cells (Vero) were investigated. It was found that the infected cells showed progressive cellular morphological changes characteristic in apoptotic cells within 10 hours post-infection. The number of apoptotic cells correlated significantly with the number of HEV71 antigen positive cells when cells were labeled using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and stained for HEV71 antigen. Approximately 11, 26, 45 and 50 of the infected cells were apoptotic at 12, 24, 48 and 72 hours post-infection, respectively. Internucleosomal DNA fragmentation, characteristic in the late stage of apoptosis was noted beginning on day 2 post-infection. The DNA fragmentation, however, was absent in cells treated with the heat- and ultraviolet light-inactivated virus inocula. These results demonstrate the capacity of HEV71 to induce apoptosis in the infected cells. The induction, however, requires high level of HEV71 infectivity and the presence of live virus particles, suggesting the need for the presence of specific viral proteins for apoptosis to occur. 2003 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3999/1/Enterovirus_71_infection_induces_apoptosis_in_Vero_cells.pdf Chan, Y.F. and AbuBakar, Sazaly (2003) Enterovirus 71 Infection Induces Apoptosis in Vero Cells. Malaysian Journal of Pathology, 25 (1). pp. 29-35. ISSN 0126-8635 (Print) 0126-8635 (Linking), DOI 16196375. http://www.ncbi.nlm.nih.gov/pubmed/16196375 16196375 |
| spellingShingle | R Medicine Chan, Y.F. AbuBakar, Sazaly Enterovirus 71 Infection Induces Apoptosis in Vero Cells |
| title | Enterovirus 71 Infection Induces Apoptosis in Vero Cells |
| title_full | Enterovirus 71 Infection Induces Apoptosis in Vero Cells |
| title_fullStr | Enterovirus 71 Infection Induces Apoptosis in Vero Cells |
| title_full_unstemmed | Enterovirus 71 Infection Induces Apoptosis in Vero Cells |
| title_short | Enterovirus 71 Infection Induces Apoptosis in Vero Cells |
| title_sort | enterovirus 71 infection induces apoptosis in vero cells |
| topic | R Medicine |
| url | http://eprints.um.edu.my/3999/1/Enterovirus_71_infection_induces_apoptosis_in_Vero_cells.pdf |
| work_keys_str_mv | AT chanyf enterovirus71infectioninducesapoptosisinverocells AT abubakarsazaly enterovirus71infectioninducesapoptosisinverocells |