Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells
Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn2+ but not Ca2+, Mg2+ or Mn2+ to the cell culture medium hastened the detection of apoptosis to within 4 h after infect...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2002
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/4007/1/Shafee-2002-Zinc_accelerates_den.pdf |
| _version_ | 1825718882163228672 |
|---|---|
| author | Shafee, N. AbuBakar, Sazaly |
| author_facet | Shafee, N. AbuBakar, Sazaly |
| author_sort | Shafee, N. |
| collection | UM |
| description | Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn2+ but not Ca2+, Mg2+ or Mn2+ to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated with Zn2+ alone or infected with heat- or ultraviolet light-inactivated DENV-2 in the presence of Zn2+. These results suggest that (i) apoptosis is induced in African green monkey kidney cells infected with live DENV-2 and (ii) the addition of high extracellular Zn2+ accelerates detection of apoptosis in the DENV-2-infected cells. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| first_indexed | 2024-03-06T05:11:07Z |
| format | Article |
| id | um.eprints-4007 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:11:07Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | um.eprints-40072019-02-13T08:03:45Z http://eprints.um.edu.my/4007/ Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells Shafee, N. AbuBakar, Sazaly R Medicine Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn2+ but not Ca2+, Mg2+ or Mn2+ to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated with Zn2+ alone or infected with heat- or ultraviolet light-inactivated DENV-2 in the presence of Zn2+. These results suggest that (i) apoptosis is induced in African green monkey kidney cells infected with live DENV-2 and (ii) the addition of high extracellular Zn2+ accelerates detection of apoptosis in the DENV-2-infected cells. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. 2002 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4007/1/Shafee-2002-Zinc_accelerates_den.pdf Shafee, N. and AbuBakar, Sazaly (2002) Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells. Febs Letters, 524 (1-3). pp. 20-24. ISSN 0014-5793, DOI https://doi.org/10.1016/s0014-5793(02)02991-5 <https://doi.org/10.1016/s0014-5793(02)02991-5>. 10.1016/s0014-5793(02)02991-5 |
| spellingShingle | R Medicine Shafee, N. AbuBakar, Sazaly Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells |
| title | Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells |
| title_full | Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells |
| title_fullStr | Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells |
| title_full_unstemmed | Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells |
| title_short | Zinc accelerates Dengue Virus Type 2-Induced Aapoptosis in Vero Cells |
| title_sort | zinc accelerates dengue virus type 2 induced aapoptosis in vero cells |
| topic | R Medicine |
| url | http://eprints.um.edu.my/4007/1/Shafee-2002-Zinc_accelerates_den.pdf |
| work_keys_str_mv | AT shafeen zincacceleratesdenguevirustype2inducedaapoptosisinverocells AT abubakarsazaly zincacceleratesdenguevirustype2inducedaapoptosisinverocells |