Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia
Microsporidia are ubiquitous parasites thought to be closely related to fungi. Their presence in the environment means that humans are frequently exposed to infection. Stool samples were collected from 151 indigenous villagers from the eastern state of Pahang in 2005. The samples were concentrated w...
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| Format: | Article |
| Language: | English |
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2010
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| Online Access: | http://eprints.um.edu.my/4028/1/Lono-2010-Prevalence_of_micros.pdf |
| _version_ | 1825718886716145664 |
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| author | Lono, A. Kumar, G.S. Chye, T.T. |
| author_facet | Lono, A. Kumar, G.S. Chye, T.T. |
| author_sort | Lono, A. |
| collection | UM |
| description | Microsporidia are ubiquitous parasites thought to be closely related to fungi. Their presence in the environment means that humans are frequently exposed to infection. Stool samples were collected from 151 indigenous villagers from the eastern state of Pahang in 2005. The samples were concentrated with water-ether sedimentation, stained with modified trichrome stain and examined under oil-immersion microscopy. Thirty-two specimens (21.2) were positive for microsporidia. Microsporidia were observed as ovoid or rounded ovoid shapes measuring similar to 1 mu m, with a bright pink outline containing a central or posterior vacuole. PCR amplification with specific primers on microscopy-positive specimens amplified Encephalitozoon intestinalis DNA from five of the ten specimens used. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved. |
| first_indexed | 2024-03-06T05:11:10Z |
| format | Article |
| id | um.eprints-4028 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:11:10Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | um.eprints-40282012-12-03T01:11:35Z http://eprints.um.edu.my/4028/ Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia Lono, A. Kumar, G.S. Chye, T.T. R Medicine Microsporidia are ubiquitous parasites thought to be closely related to fungi. Their presence in the environment means that humans are frequently exposed to infection. Stool samples were collected from 151 indigenous villagers from the eastern state of Pahang in 2005. The samples were concentrated with water-ether sedimentation, stained with modified trichrome stain and examined under oil-immersion microscopy. Thirty-two specimens (21.2) were positive for microsporidia. Microsporidia were observed as ovoid or rounded ovoid shapes measuring similar to 1 mu m, with a bright pink outline containing a central or posterior vacuole. PCR amplification with specific primers on microscopy-positive specimens amplified Encephalitozoon intestinalis DNA from five of the ten specimens used. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4028/1/Lono-2010-Prevalence_of_micros.pdf Lono, A. and Kumar, G.S. and Chye, T.T. (2010) Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 104 (3). pp. 214-218. ISSN 0035-9203, DOI https://doi.org/10.1016/j.trstmh.2009.07.006 <https://doi.org/10.1016/j.trstmh.2009.07.006>. http://ac.els-cdn.com/S0035920309002326/1-s2.0-S0035920309002326-main.pdf?_tid=05b837ea-1671-11e2-bf6e-00000aab0f27&acdnat=1350268725_295f8c37024f44c5ddd8c89a8fccdf6d 10.1016/j.trstmh.2009.07.006 |
| spellingShingle | R Medicine Lono, A. Kumar, G.S. Chye, T.T. Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia |
| title | Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia |
| title_full | Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia |
| title_fullStr | Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia |
| title_full_unstemmed | Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia |
| title_short | Prevalence of microsporidia in an indigenous Orang Asli community in Pahang, Malaysia |
| title_sort | prevalence of microsporidia in an indigenous orang asli community in pahang malaysia |
| topic | R Medicine |
| url | http://eprints.um.edu.my/4028/1/Lono-2010-Prevalence_of_micros.pdf |
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