Species identification of intestinal microsporidia using immunofluorescence antibody assays
The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), molecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spore...
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| Format: | Article |
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SEAMEO Regional Tropical Medicine and Public Health Network
2011
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| author | Al-Mekhlafi, M.A. Fatmah, M.S. Anisah, N. Azlin, M. Al-Mekhlafi, H.M. Norhayati, M. |
| author_facet | Al-Mekhlafi, M.A. Fatmah, M.S. Anisah, N. Azlin, M. Al-Mekhlafi, H.M. Norhayati, M. |
| author_sort | Al-Mekhlafi, M.A. |
| collection | UM |
| description | The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), molecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spores using the Weber modified trichrome (WMT) staining technique were examined using IFA-MAbs. Of the 100 specimens examined, the microsporidial spores identified by IFA-MAbs were Enterocytozoon bieneusi 42 (75) Encephalitozoon intestinalis 7(12.5) and mixed infections 7 (12.5). The sensitivity and specificity of IFA-MAbs in detecting microsporidial spores were 98 and 86, respectively. The agreement between the WMT staining technique and IFA-MAbs was statistically significant by Kappa statistics (K=0.840; p<0.001). E. bieneusi was the commonest Microsporidia species isolated from the studied population; the presence of microsporidial spores detected by IFA-MAbs should be confirmed by other methods. |
| first_indexed | 2024-03-06T05:11:28Z |
| format | Article |
| id | um.eprints-4170 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:11:28Z |
| publishDate | 2011 |
| publisher | SEAMEO Regional Tropical Medicine and Public Health Network |
| record_format | dspace |
| spelling | um.eprints-41702021-04-12T03:53:02Z http://eprints.um.edu.my/4170/ Species identification of intestinal microsporidia using immunofluorescence antibody assays Al-Mekhlafi, M.A. Fatmah, M.S. Anisah, N. Azlin, M. Al-Mekhlafi, H.M. Norhayati, M. R Medicine The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), molecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spores using the Weber modified trichrome (WMT) staining technique were examined using IFA-MAbs. Of the 100 specimens examined, the microsporidial spores identified by IFA-MAbs were Enterocytozoon bieneusi 42 (75) Encephalitozoon intestinalis 7(12.5) and mixed infections 7 (12.5). The sensitivity and specificity of IFA-MAbs in detecting microsporidial spores were 98 and 86, respectively. The agreement between the WMT staining technique and IFA-MAbs was statistically significant by Kappa statistics (K=0.840; p<0.001). E. bieneusi was the commonest Microsporidia species isolated from the studied population; the presence of microsporidial spores detected by IFA-MAbs should be confirmed by other methods. SEAMEO Regional Tropical Medicine and Public Health Network 2011 Article PeerReviewed Al-Mekhlafi, M.A. and Fatmah, M.S. and Anisah, N. and Azlin, M. and Al-Mekhlafi, H.M. and Norhayati, M. (2011) Species identification of intestinal microsporidia using immunofluorescence antibody assays. The Southeast Asian Journal of Tropical Medicine and Public Health, 42 (1). pp. 19-24. ISSN 0125-1562, https://www.tm.mahidol.ac.th/seameo/2011-42-1/03-4886.pdf |
| spellingShingle | R Medicine Al-Mekhlafi, M.A. Fatmah, M.S. Anisah, N. Azlin, M. Al-Mekhlafi, H.M. Norhayati, M. Species identification of intestinal microsporidia using immunofluorescence antibody assays |
| title | Species identification of intestinal microsporidia using immunofluorescence antibody assays |
| title_full | Species identification of intestinal microsporidia using immunofluorescence antibody assays |
| title_fullStr | Species identification of intestinal microsporidia using immunofluorescence antibody assays |
| title_full_unstemmed | Species identification of intestinal microsporidia using immunofluorescence antibody assays |
| title_short | Species identification of intestinal microsporidia using immunofluorescence antibody assays |
| title_sort | species identification of intestinal microsporidia using immunofluorescence antibody assays |
| topic | R Medicine |
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