Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study
Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using alpha chain-specific mon...
| Principais autores: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Wiley
2002
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| Assuntos: | |
| Acesso em linha: | http://eprints.um.edu.my/4296/1/Distribution_of_basement_membrane_type_IV_collagen_alpha_chains_in_ameloblastoma_an_immunofluorescence_study.pdf |
| _version_ | 1825718923041964032 |
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| author | Nakano, K. Siar, C.H. Nagai, N. Naito, I. Sado, Y. Nagatsuka, H. Hoh, C. Kurada, K. Tsujigiwa, H. Gunduz, M. |
| author_facet | Nakano, K. Siar, C.H. Nagai, N. Naito, I. Sado, Y. Nagatsuka, H. Hoh, C. Kurada, K. Tsujigiwa, H. Gunduz, M. |
| author_sort | Nakano, K. |
| collection | UM |
| description | Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using alpha chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; alpha4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed alpha1(IV), alpha2(IV), and alpha4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed alpha1(V), alpha2(IV), alpha5(IV), and alpha6(IV) chains. Conclusions: Results suggest that collagen IV alpha chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression. |
| first_indexed | 2024-03-06T05:11:42Z |
| format | Article |
| id | um.eprints-4296 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2025-03-05T02:20:45Z |
| publishDate | 2002 |
| publisher | Wiley |
| record_format | dspace |
| spelling | um.eprints-42962024-11-15T03:53:11Z http://eprints.um.edu.my/4296/ Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study Nakano, K. Siar, C.H. Nagai, N. Naito, I. Sado, Y. Nagatsuka, H. Hoh, C. Kurada, K. Tsujigiwa, H. Gunduz, M. RK Dentistry Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using alpha chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; alpha4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed alpha1(IV), alpha2(IV), and alpha4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed alpha1(V), alpha2(IV), alpha5(IV), and alpha6(IV) chains. Conclusions: Results suggest that collagen IV alpha chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression. Wiley 2002 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4296/1/Distribution_of_basement_membrane_type_IV_collagen_alpha_chains_in_ameloblastoma_an_immunofluorescence_study.pdf Nakano, K. and Siar, C.H. and Nagai, N. and Naito, I. and Sado, Y. and Nagatsuka, H. and Hoh, C. and Kurada, K. and Tsujigiwa, H. and Gunduz, M. (2002) Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study. Journal of Oral Pathology & Medicine, 31 (8). pp. 494-499. ISSN 0904-2512, DOI https://doi.org/10.1034/j.1600-0714.2002.00162.x <https://doi.org/10.1034/j.1600-0714.2002.00162.x>. 10.1034/j.1600-0714.2002.00162.x |
| spellingShingle | RK Dentistry Nakano, K. Siar, C.H. Nagai, N. Naito, I. Sado, Y. Nagatsuka, H. Hoh, C. Kurada, K. Tsujigiwa, H. Gunduz, M. Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study |
| title | Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study |
| title_full | Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study |
| title_fullStr | Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study |
| title_full_unstemmed | Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study |
| title_short | Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study |
| title_sort | distribution of basement membrane type iv collagen alpha chains in ameloblastoma an immunofluorescence study |
| topic | RK Dentistry |
| url | http://eprints.um.edu.my/4296/1/Distribution_of_basement_membrane_type_IV_collagen_alpha_chains_in_ameloblastoma_an_immunofluorescence_study.pdf |
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