Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor

Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs) are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites). Although GCs are present in a variety of oc...

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Main Authors: Siar, C.H., Kawakami, T., Buery, R.R., Nakano, K., Tomida, M., Tsujigiwa, H., Han, P.P., Nagatsuka, H., Ng, K.H.
Format: Article
Language:English
Published: 2011
Subjects:
Online Access:http://eprints.um.edu.my/4323/1/Notch_signaling_and_ghost_cell_fate_in_the_calcifying_cystic_odontogenic_tumor.pdf
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author Siar, C.H.
Kawakami, T.
Buery, R.R.
Nakano, K.
Tomida, M.
Tsujigiwa, H.
Han, P.P.
Nagatsuka, H.
Ng, K.H.
author_facet Siar, C.H.
Kawakami, T.
Buery, R.R.
Nakano, K.
Tomida, M.
Tsujigiwa, H.
Han, P.P.
Nagatsuka, H.
Ng, K.H.
author_sort Siar, C.H.
collection UM
description Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs) are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites). Although GCs are present in a variety of oclontogenic lesions notably the calcifying cystic odontogenic tumor (CCOT), their nature and process of formation remains elusive. The aim of this study was to investigate the role of Notch signaling in the cell fate specification of GCs in CCOT. Immunohistochemical staining for four Notch receptors (Notchl, Notch2, Notch3 and Notch4) and three ligands (jagged1, jagged2 and Delta)) was performed on archival tissues of five CCOT cases. Level of positivity was quantified as negative (0), mild (+), moderate (2+) and strong (3+). Results revealed that GCs demonstrated overexpression for Notchl and jaggedl suggesting that Notchljaggedl signaling might serve as the main transduction mechanism in cell fate decision for GCs in CCOT. Protein localizations were largely membranous and/or cytoplasmic. Mineralized GCs also stained positive implicating that the calcification process might be associated with upregulation of these molecules. The other Notch receptors and lig-ands were weak to absent in GCs and tumoral epithelium. Stromal endothelium and fibroblasts were stained variably positive.
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spelling um.eprints-43232013-01-10T00:02:44Z http://eprints.um.edu.my/4323/ Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor Siar, C.H. Kawakami, T. Buery, R.R. Nakano, K. Tomida, M. Tsujigiwa, H. Han, P.P. Nagatsuka, H. Ng, K.H. RK Dentistry Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs) are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites). Although GCs are present in a variety of oclontogenic lesions notably the calcifying cystic odontogenic tumor (CCOT), their nature and process of formation remains elusive. The aim of this study was to investigate the role of Notch signaling in the cell fate specification of GCs in CCOT. Immunohistochemical staining for four Notch receptors (Notchl, Notch2, Notch3 and Notch4) and three ligands (jagged1, jagged2 and Delta)) was performed on archival tissues of five CCOT cases. Level of positivity was quantified as negative (0), mild (+), moderate (2+) and strong (3+). Results revealed that GCs demonstrated overexpression for Notchl and jaggedl suggesting that Notchljaggedl signaling might serve as the main transduction mechanism in cell fate decision for GCs in CCOT. Protein localizations were largely membranous and/or cytoplasmic. Mineralized GCs also stained positive implicating that the calcification process might be associated with upregulation of these molecules. The other Notch receptors and lig-ands were weak to absent in GCs and tumoral epithelium. Stromal endothelium and fibroblasts were stained variably positive. 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4323/1/Notch_signaling_and_ghost_cell_fate_in_the_calcifying_cystic_odontogenic_tumor.pdf Siar, C.H. and Kawakami, T. and Buery, R.R. and Nakano, K. and Tomida, M. and Tsujigiwa, H. and Han, P.P. and Nagatsuka, H. and Ng, K.H. (2011) Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor. European Journal of Medical Research, 16 (11). pp. 501-506. ISSN 0949-2321,
spellingShingle RK Dentistry
Siar, C.H.
Kawakami, T.
Buery, R.R.
Nakano, K.
Tomida, M.
Tsujigiwa, H.
Han, P.P.
Nagatsuka, H.
Ng, K.H.
Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
title Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
title_full Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
title_fullStr Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
title_full_unstemmed Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
title_short Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
title_sort notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor
topic RK Dentistry
url http://eprints.um.edu.my/4323/1/Notch_signaling_and_ghost_cell_fate_in_the_calcifying_cystic_odontogenic_tumor.pdf
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