Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma
BACKGROUND: Canonical and non-canonical Wnt signaling pathways modulate diverse cellular processes during embryogenesis and post-natally. Their deregulations have been implicated in cancer development and progression. Wnt signaling is essential for odontogenesis. The ameloblastoma is an odontogenic...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
2012
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/4329/1/Differential_expression_of_canonical_and_non-canonical_Wnt_ligands_in_ameloblastoma.pdf http://eprints.um.edu.my/4329/2/Differential_expression_of_canonical_and_non-canonical_Wnt_ligands_in_ameloblastoma.pdf |
| _version_ | 1796945110849552384 |
|---|---|
| author | Siar, C.H. Nagatsuka, H. Han, P.P. Buery, R.R. Tsujigiwa, H. Nakano, K. Ng, K.H. Kawakami, T. |
| author_facet | Siar, C.H. Nagatsuka, H. Han, P.P. Buery, R.R. Tsujigiwa, H. Nakano, K. Ng, K.H. Kawakami, T. |
| author_sort | Siar, C.H. |
| collection | UM |
| description | BACKGROUND: Canonical and non-canonical Wnt signaling pathways modulate diverse cellular processes during embryogenesis and post-natally. Their deregulations have been implicated in cancer development and progression. Wnt signaling is essential for odontogenesis. The ameloblastoma is an odontogenic epithelial neoplasm of enamel organ origin. Altered expressions of Wnts-1, -2, -5a, and -10a are detected in this tumor. The activity of other Wnt members remains unclarified. MATERIALS AND METHODS: Canonical (Wnts-1, -2, -3, -8a, -8b, -10a, and -10b), non-canonical (Wnts-4, -5a, -5b, -6, 7a, -7b, and -11), and indeterminate groups (Wnts-2b and -9b) were examined immunohistochemically in 72 cases of ameloblastoma (19 unicystic UA, 35 solid/multicystic SMA, eight desmoplastic DA, and 10 recurrent RA). RESULTS: Canonical Wnt proteins (except Wnt-10b) were heterogeneously expressed in ameloblastoma. Their distribution patterns were distinctive with some overlap. Protein localization was mainly membranous and/or cytoplasmic. Overexpression of Wnt-1 in most subsets (UA = 19/19; SMA = 35/35; DA = 5/8; RA = 7/10) (P < 0.05), Wnt-3 in granular cell variant (n = 3/ 3), and Wnt-8b in DA (n = 8/ 8) was key observations. Wnts-8a and -10a demonstrated enhanced expression in tumoral buddings and acanthomatous areas. Noncanonical and indeterminate Wnts were absent except for limited Wnt-7b immunoreactivity in UA (n = 1/ 19) and SMA (n = 1/ 35). Stromal components expressed variable Wnt positivity. CONCLUSION: Differential expression of Wnt ligands in different ameloblastoma subtypes suggests that the canonical and non-canonical Wnt pathways are selectively activated or repressed depending on the tumor cell differentiation status. Canonical Wnt pathway is most likely the main transduction pathway while Wnt-1 might be the key signaling molecule involved in ameloblastoma tumorigenesis. J Oral Pathol Med (2012) 41: 332-339 |
| first_indexed | 2024-03-06T05:11:46Z |
| format | Article |
| id | um.eprints-4329 |
| institution | Universiti Malaya |
| language | English English |
| last_indexed | 2024-03-06T05:11:46Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | um.eprints-43292013-01-10T00:23:59Z http://eprints.um.edu.my/4329/ Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma Siar, C.H. Nagatsuka, H. Han, P.P. Buery, R.R. Tsujigiwa, H. Nakano, K. Ng, K.H. Kawakami, T. RK Dentistry BACKGROUND: Canonical and non-canonical Wnt signaling pathways modulate diverse cellular processes during embryogenesis and post-natally. Their deregulations have been implicated in cancer development and progression. Wnt signaling is essential for odontogenesis. The ameloblastoma is an odontogenic epithelial neoplasm of enamel organ origin. Altered expressions of Wnts-1, -2, -5a, and -10a are detected in this tumor. The activity of other Wnt members remains unclarified. MATERIALS AND METHODS: Canonical (Wnts-1, -2, -3, -8a, -8b, -10a, and -10b), non-canonical (Wnts-4, -5a, -5b, -6, 7a, -7b, and -11), and indeterminate groups (Wnts-2b and -9b) were examined immunohistochemically in 72 cases of ameloblastoma (19 unicystic UA, 35 solid/multicystic SMA, eight desmoplastic DA, and 10 recurrent RA). RESULTS: Canonical Wnt proteins (except Wnt-10b) were heterogeneously expressed in ameloblastoma. Their distribution patterns were distinctive with some overlap. Protein localization was mainly membranous and/or cytoplasmic. Overexpression of Wnt-1 in most subsets (UA = 19/19; SMA = 35/35; DA = 5/8; RA = 7/10) (P < 0.05), Wnt-3 in granular cell variant (n = 3/ 3), and Wnt-8b in DA (n = 8/ 8) was key observations. Wnts-8a and -10a demonstrated enhanced expression in tumoral buddings and acanthomatous areas. Noncanonical and indeterminate Wnts were absent except for limited Wnt-7b immunoreactivity in UA (n = 1/ 19) and SMA (n = 1/ 35). Stromal components expressed variable Wnt positivity. CONCLUSION: Differential expression of Wnt ligands in different ameloblastoma subtypes suggests that the canonical and non-canonical Wnt pathways are selectively activated or repressed depending on the tumor cell differentiation status. Canonical Wnt pathway is most likely the main transduction pathway while Wnt-1 might be the key signaling molecule involved in ameloblastoma tumorigenesis. J Oral Pathol Med (2012) 41: 332-339 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4329/1/Differential_expression_of_canonical_and_non-canonical_Wnt_ligands_in_ameloblastoma.pdf application/pdf en http://eprints.um.edu.my/4329/2/Differential_expression_of_canonical_and_non-canonical_Wnt_ligands_in_ameloblastoma.pdf Siar, C.H. and Nagatsuka, H. and Han, P.P. and Buery, R.R. and Tsujigiwa, H. and Nakano, K. and Ng, K.H. and Kawakami, T. (2012) Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma. Journal of Oral Pathology & Medicine, 41 (4). pp. 332-339. ISSN 0904-2512, DOI https://doi.org/10.1111/j.1600-0714.2011.01104.x <https://doi.org/10.1111/j.1600-0714.2011.01104.x>. 10.1111/j.1600-0714.2011.01104.x |
| spellingShingle | RK Dentistry Siar, C.H. Nagatsuka, H. Han, P.P. Buery, R.R. Tsujigiwa, H. Nakano, K. Ng, K.H. Kawakami, T. Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma |
| title | Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma |
| title_full | Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma |
| title_fullStr | Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma |
| title_full_unstemmed | Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma |
| title_short | Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma |
| title_sort | differential expression of canonical and non canonical wnt ligands in ameloblastoma |
| topic | RK Dentistry |
| url | http://eprints.um.edu.my/4329/1/Differential_expression_of_canonical_and_non-canonical_Wnt_ligands_in_ameloblastoma.pdf http://eprints.um.edu.my/4329/2/Differential_expression_of_canonical_and_non-canonical_Wnt_ligands_in_ameloblastoma.pdf |
| work_keys_str_mv | AT siarch differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT nagatsukah differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT hanpp differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT bueryrr differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT tsujigiwah differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT nakanok differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT ngkh differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma AT kawakamit differentialexpressionofcanonicalandnoncanonicalwntligandsinameloblastoma |