Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia
Objectives: To investigate the molecular epidemiology of HIV-1 and to screen for the emergence of intersubtype recombinants in Kuala Lumpur, Malaysia. Design: A molecular epidemiology study was conducted among HIV-1 seropositive patients attending the University Malaya Medical Center (UMMC) from Jul...
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| Format: | Article |
| Language: | English |
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2005
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| Online Access: | http://eprints.um.edu.my/4560/1/Emergence_of_HIV-1_CRF01_AE-B_unique_recombinant_forms_in_Kuala_Lumpur%2C_Malaysia.pdf |
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| author | Tee, K.K. Pon, C.K. Kamarulzaman, A. Ng, K.P. |
| author_facet | Tee, K.K. Pon, C.K. Kamarulzaman, A. Ng, K.P. |
| author_sort | Tee, K.K. |
| collection | UM |
| description | Objectives: To investigate the molecular epidemiology of HIV-1 and to screen for the emergence of intersubtype recombinants in Kuala Lumpur, Malaysia. Design: A molecular epidemiology study was conducted among HIV-1 seropositive patients attending the University Malaya Medical Center (UMMC) from July 2003 to June 2004. Methods: Protease (PR) and reverse transcriptase (RT) gene sequences were derived from drug resistance genotyping assay of 100 newly diagnosed or antiretroviral-naive patients. These were phylogenetically analysed to determine the subtypes and recombination breakpoint analyses were performed on intersubtype recombinants to estimate the recombination breakpoint(s). Results: CRF01AE predominated in Kuala Lumpur with 65 in both PR and RT genes. B subtype was detected at 14 and 12 in PR and RT genes, respectively. C subtype was present at 1 in both genes. Overall, the concordance of PR and RT genes in discriminating subtypes/circulating recombinant forms (CRF) was high at 96. In this study, novel CRF01AE/B intersubtype recombinants were detected at high prevalence (22), including those isolates with subtype discordance. Thai variants of CRF01AE and B subtype were involved in the genesis of these unique recombinant forms (URF). Interestingly, 19 CRF01AE/B intersubtype recombinant isolates shared similar recombination breakpoints in both PR and RT genes. Several distinct URF were also identified. Conclusion: PR and RT genes can be utilized for subtype/CRF assessment with high degree of agreement, allowing concurrent surveillance of circulating HIV-1 subtypes with antiretroviral drug resistance genotyping tests. The emergence of highly identical CRF01AE/B intersubtype recombinants suggests the possibility of the appearance of a new circulating recombinant form in Kuala Lumpur. (c) 2005 Lippincott Williams C Wilkins. |
| first_indexed | 2024-03-06T05:12:16Z |
| format | Article |
| id | um.eprints-4560 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:12:16Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | um.eprints-45602013-03-13T08:11:53Z http://eprints.um.edu.my/4560/ Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia Tee, K.K. Pon, C.K. Kamarulzaman, A. Ng, K.P. R Medicine Objectives: To investigate the molecular epidemiology of HIV-1 and to screen for the emergence of intersubtype recombinants in Kuala Lumpur, Malaysia. Design: A molecular epidemiology study was conducted among HIV-1 seropositive patients attending the University Malaya Medical Center (UMMC) from July 2003 to June 2004. Methods: Protease (PR) and reverse transcriptase (RT) gene sequences were derived from drug resistance genotyping assay of 100 newly diagnosed or antiretroviral-naive patients. These were phylogenetically analysed to determine the subtypes and recombination breakpoint analyses were performed on intersubtype recombinants to estimate the recombination breakpoint(s). Results: CRF01AE predominated in Kuala Lumpur with 65 in both PR and RT genes. B subtype was detected at 14 and 12 in PR and RT genes, respectively. C subtype was present at 1 in both genes. Overall, the concordance of PR and RT genes in discriminating subtypes/circulating recombinant forms (CRF) was high at 96. In this study, novel CRF01AE/B intersubtype recombinants were detected at high prevalence (22), including those isolates with subtype discordance. Thai variants of CRF01AE and B subtype were involved in the genesis of these unique recombinant forms (URF). Interestingly, 19 CRF01AE/B intersubtype recombinant isolates shared similar recombination breakpoints in both PR and RT genes. Several distinct URF were also identified. Conclusion: PR and RT genes can be utilized for subtype/CRF assessment with high degree of agreement, allowing concurrent surveillance of circulating HIV-1 subtypes with antiretroviral drug resistance genotyping tests. The emergence of highly identical CRF01AE/B intersubtype recombinants suggests the possibility of the appearance of a new circulating recombinant form in Kuala Lumpur. (c) 2005 Lippincott Williams C Wilkins. 2005 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4560/1/Emergence_of_HIV-1_CRF01_AE-B_unique_recombinant_forms_in_Kuala_Lumpur%2C_Malaysia.pdf Tee, K.K. and Pon, C.K. and Kamarulzaman, A. and Ng, K.P. (2005) Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia. AIDS, 19 (2). pp. 119-126. ISSN 0269-9370, |
| spellingShingle | R Medicine Tee, K.K. Pon, C.K. Kamarulzaman, A. Ng, K.P. Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia |
| title | Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia |
| title_full | Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia |
| title_fullStr | Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia |
| title_full_unstemmed | Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia |
| title_short | Emergence of HIV-1 CRF01AE/B unique recombinant forms in Kuala Lumpur, Malaysia |
| title_sort | emergence of hiv 1 crf01ae b unique recombinant forms in kuala lumpur malaysia |
| topic | R Medicine |
| url | http://eprints.um.edu.my/4560/1/Emergence_of_HIV-1_CRF01_AE-B_unique_recombinant_forms_in_Kuala_Lumpur%2C_Malaysia.pdf |
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