Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B
In Malaysia, co-circulation of CRF01_AE and subtype B has resulted in the emergence of the second generation derivative; CRF33_01B in approximately 20% of its HIV-1 infected individuals. Our objective was to identify possible biological advantages that CRF33_01B possesses over its progenitors. Biolo...
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Bentham Science Publishers
2010
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| author | Lau, Katherine A. Wang, Bin Miranda-Saksena, Monica Boadle, Ross Kamarulzaman, Adeeba Ng, Kee Peng Saksena, Nitin K. |
| author_facet | Lau, Katherine A. Wang, Bin Miranda-Saksena, Monica Boadle, Ross Kamarulzaman, Adeeba Ng, Kee Peng Saksena, Nitin K. |
| author_sort | Lau, Katherine A. |
| collection | UM |
| description | In Malaysia, co-circulation of CRF01_AE and subtype B has resulted in the emergence of the second generation derivative; CRF33_01B in approximately 20% of its HIV-1 infected individuals. Our objective was to identify possible biological advantages that CRF33_01B possesses over its progenitors. Biological and molecular comparisons of CRF33_01B against its parental subtypes clearly show that CRF33_01B replicated better in activated whole peripheral blood mononuclear cells (PBMCs) and CD4+ T-lymphocytes, but not monocyte-derived macrophages (MDMs). Also, its acquired fitness was greater than CRF01_AE but not subtype B. Moreover, CRF33_01B has higher rate of apoptotic cell death and syncytia induction compared to subtype B. These adaptive and survival abilities could have been acquired by CRF33_01B due to the incorporation of subtype B fragments into the gag-RT region of its full-length genome. Our studies confirm the previously held belief that HIV-1 strains may harbor enhanced biological fitness upon recombination. We therefore estimate a possible gradual replacement of the current predominance of CRF01_AE, as well as wider dissemination of CRF33_01B, together with the identification of other new CRF01_AE/B inter-subtype recombinants in Malaysia. |
| first_indexed | 2024-03-06T05:12:17Z |
| format | Article |
| id | um.eprints-4568 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:12:17Z |
| publishDate | 2010 |
| publisher | Bentham Science Publishers |
| record_format | dspace |
| spelling | um.eprints-45682019-11-11T05:11:30Z http://eprints.um.edu.my/4568/ Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B Lau, Katherine A. Wang, Bin Miranda-Saksena, Monica Boadle, Ross Kamarulzaman, Adeeba Ng, Kee Peng Saksena, Nitin K. R Medicine In Malaysia, co-circulation of CRF01_AE and subtype B has resulted in the emergence of the second generation derivative; CRF33_01B in approximately 20% of its HIV-1 infected individuals. Our objective was to identify possible biological advantages that CRF33_01B possesses over its progenitors. Biological and molecular comparisons of CRF33_01B against its parental subtypes clearly show that CRF33_01B replicated better in activated whole peripheral blood mononuclear cells (PBMCs) and CD4+ T-lymphocytes, but not monocyte-derived macrophages (MDMs). Also, its acquired fitness was greater than CRF01_AE but not subtype B. Moreover, CRF33_01B has higher rate of apoptotic cell death and syncytia induction compared to subtype B. These adaptive and survival abilities could have been acquired by CRF33_01B due to the incorporation of subtype B fragments into the gag-RT region of its full-length genome. Our studies confirm the previously held belief that HIV-1 strains may harbor enhanced biological fitness upon recombination. We therefore estimate a possible gradual replacement of the current predominance of CRF01_AE, as well as wider dissemination of CRF33_01B, together with the identification of other new CRF01_AE/B inter-subtype recombinants in Malaysia. Bentham Science Publishers 2010 Article PeerReviewed Lau, Katherine A. and Wang, Bin and Miranda-Saksena, Monica and Boadle, Ross and Kamarulzaman, Adeeba and Ng, Kee Peng and Saksena, Nitin K. (2010) Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B. Current HIV Research, 8 (3). pp. 259-271. ISSN 1570-162X, DOI https://doi.org/10.2174/157016210791111151 <https://doi.org/10.2174/157016210791111151>. https://doi.org/10.2174/157016210791111151 doi:10.2174/157016210791111151 |
| spellingShingle | R Medicine Lau, Katherine A. Wang, Bin Miranda-Saksena, Monica Boadle, Ross Kamarulzaman, Adeeba Ng, Kee Peng Saksena, Nitin K. Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B |
| title | Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B |
| title_full | Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B |
| title_fullStr | Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B |
| title_full_unstemmed | Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B |
| title_short | Evidence for Possible Biological Advantages of the Newly Emerging HIV-1 Circulating Recombinant Form from Malaysia - CRF33_01B in Comparison to its Progenitors _ CRF01_AE and Subtype B |
| title_sort | evidence for possible biological advantages of the newly emerging hiv 1 circulating recombinant form from malaysia crf33 01b in comparison to its progenitors crf01 ae and subtype b |
| topic | R Medicine |
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