Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia
A molecular epidemiological investigation was conducted among various risk populations (n = 184) in Kuala Lumpur, Malaysia, in 2003 to 2005, on the basis of nucleotide sequences of protease and reverse transcriptase regions. In addition to circulating HIV-1 strains, including CRF01AE (57.1), subtype...
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Lippincott, Williams & Wilkins
2006
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| author | Tee, K.K. Li, X.J. Nohtomi, K. Ng, K.P. Kamarulzaman, Adeeba Takebe, Y. |
| author_facet | Tee, K.K. Li, X.J. Nohtomi, K. Ng, K.P. Kamarulzaman, Adeeba Takebe, Y. |
| author_sort | Tee, K.K. |
| collection | UM |
| description | A molecular epidemiological investigation was conducted among various risk populations (n = 184) in Kuala Lumpur, Malaysia, in 2003 to 2005, on the basis of nucleotide sequences of protease and reverse transcriptase regions. In addition to circulating HIV-1 strains, including CRF01AE (57.1), subtype B (20.1), and subtype C (0.5), we detected a candidate with a new circulating recombinant form (CRF). We determined four near-full-length nucleotide sequences with identical subtype structure from epidemiologically unlinked individuals of different risk and ethnic groups. In this chimera, two short subtype B segments were inserted into the gag-RT region in a backbone of CRF01AE. The recombinant structure was distinct from previously identified CRF1501B in Thailand. In agreement with the current HIV nomenclature system, this constitutes a novel CRF (CRF3301B). The overall prevalence of CRF3301B is 19.0 (35/184). Although the prevalence of CRF3301B is particularly high among injecting drug users (42.0, 21/50), it is also detected in a substantial proportion of homo-/bisexual males (18.8, 3/16) and heterosexuals (9.8, 9/92). Moreover, unique recombinant forms composed of CRF01AE and subtype B that have a significant structural relationship with CRF3301B were detected in 1.6 (3/184) of study subjects, suggesting an ongoing recombination process in Malaysia. This new CRF seems to be bridging viral transmission between different risk populations in this country. |
| first_indexed | 2024-03-06T05:12:20Z |
| format | Article |
| id | um.eprints-4588 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:12:20Z |
| publishDate | 2006 |
| publisher | Lippincott, Williams & Wilkins |
| record_format | dspace |
| spelling | um.eprints-45882019-03-21T04:35:31Z http://eprints.um.edu.my/4588/ Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia Tee, K.K. Li, X.J. Nohtomi, K. Ng, K.P. Kamarulzaman, Adeeba Takebe, Y. R Medicine A molecular epidemiological investigation was conducted among various risk populations (n = 184) in Kuala Lumpur, Malaysia, in 2003 to 2005, on the basis of nucleotide sequences of protease and reverse transcriptase regions. In addition to circulating HIV-1 strains, including CRF01AE (57.1), subtype B (20.1), and subtype C (0.5), we detected a candidate with a new circulating recombinant form (CRF). We determined four near-full-length nucleotide sequences with identical subtype structure from epidemiologically unlinked individuals of different risk and ethnic groups. In this chimera, two short subtype B segments were inserted into the gag-RT region in a backbone of CRF01AE. The recombinant structure was distinct from previously identified CRF1501B in Thailand. In agreement with the current HIV nomenclature system, this constitutes a novel CRF (CRF3301B). The overall prevalence of CRF3301B is 19.0 (35/184). Although the prevalence of CRF3301B is particularly high among injecting drug users (42.0, 21/50), it is also detected in a substantial proportion of homo-/bisexual males (18.8, 3/16) and heterosexuals (9.8, 9/92). Moreover, unique recombinant forms composed of CRF01AE and subtype B that have a significant structural relationship with CRF3301B were detected in 1.6 (3/184) of study subjects, suggesting an ongoing recombination process in Malaysia. This new CRF seems to be bridging viral transmission between different risk populations in this country. Lippincott, Williams & Wilkins 2006 Article PeerReviewed Tee, K.K. and Li, X.J. and Nohtomi, K. and Ng, K.P. and Kamarulzaman, Adeeba and Takebe, Y. (2006) Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia. Journal of Acquired Immune Deficiency Syndromes, 43 (5). pp. 523-529. ISSN 1525-4135, |
| spellingShingle | R Medicine Tee, K.K. Li, X.J. Nohtomi, K. Ng, K.P. Kamarulzaman, Adeeba Takebe, Y. Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia |
| title | Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia |
| title_full | Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia |
| title_fullStr | Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia |
| title_full_unstemmed | Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia |
| title_short | Identification of a novel circulating recombinant form (CRF3301B) disseminating widely among various risk populations in Kuala Lumpur, Malaysia |
| title_sort | identification of a novel circulating recombinant form crf3301b disseminating widely among various risk populations in kuala lumpur malaysia |
| topic | R Medicine |
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