Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B)
A growing number of emerging HIV-1 recombinants classified as circulating recombinant forms (CRFs) have been identified in Southeast Asia in recent years, establishing a molecular diversity of increasing complexity in the region. Here, we constructed a replication-competent HIV-1 clone for CRF3301B...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science
2009
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| Online Access: | http://eprints.um.edu.my/4594/1/Isolation_and_Characterization_of_a_Replication-Competent_Molecular_Clone_of_an_HIV-1_Circulating_Recombinant_Form_%28CRF33_01B%29.pdf |
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| author | Tee, K.K. Kusagawa, S. Li, X.J. Onogi, N. Isogai, M. Hase, S. Uenishi, R. Liao, H.N. Kamarulzaman, A. Takebe, Y. |
| author_facet | Tee, K.K. Kusagawa, S. Li, X.J. Onogi, N. Isogai, M. Hase, S. Uenishi, R. Liao, H.N. Kamarulzaman, A. Takebe, Y. |
| author_sort | Tee, K.K. |
| collection | UM |
| description | A growing number of emerging HIV-1 recombinants classified as circulating recombinant forms (CRFs) have been identified in Southeast Asia in recent years, establishing a molecular diversity of increasing complexity in the region. Here, we constructed a replication-competent HIV-1 clone for CRF3301B (designated p05MYKL045.1), a newly identified recombinant comprised of CRF01AE and subtype B. p05MYKL045.1 was reconstituted by cloning of the near full-length HIV-1 sequence from a newly-diagnosed individual presumably infected heterosexually in Kuala Lumpur, Malaysia. The chimeric clone, which contains the 59 LTR (long terminal repeat) region of p93JP-NH1 (a previously isolated CRF01AE infectious clone), showed robust viral replication in the human peripheral blood mononuclear cells. This clone demonstrated robust viral propagation and profound syncytium formation in CD4(+), CXCR4-expressing human glioma NP-2 cells, indicating that p05MYKL045.1 is a CXCR4-using virus. Viral propagation, however, was not detected in various human T cell lines including MT-2, M8166, Sup-T1, H9, Jurkat, Molt-4 and PM1.p05MYKL045.1 appears to proliferate only in restricted host range, suggesting that unknown viral and/or cellular host factors may play a role in viral infectivity and replication in human T cell lines. Availability of a CRF3301B molecular clone will be useful in facilitating the development of vaccine candidates that match the HIV-1 strains circulating in Southeast Asia. |
| first_indexed | 2024-03-06T05:12:21Z |
| format | Article |
| id | um.eprints-4594 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:12:21Z |
| publishDate | 2009 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | um.eprints-45942017-11-13T07:33:25Z http://eprints.um.edu.my/4594/ Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) Tee, K.K. Kusagawa, S. Li, X.J. Onogi, N. Isogai, M. Hase, S. Uenishi, R. Liao, H.N. Kamarulzaman, A. Takebe, Y. R Medicine A growing number of emerging HIV-1 recombinants classified as circulating recombinant forms (CRFs) have been identified in Southeast Asia in recent years, establishing a molecular diversity of increasing complexity in the region. Here, we constructed a replication-competent HIV-1 clone for CRF3301B (designated p05MYKL045.1), a newly identified recombinant comprised of CRF01AE and subtype B. p05MYKL045.1 was reconstituted by cloning of the near full-length HIV-1 sequence from a newly-diagnosed individual presumably infected heterosexually in Kuala Lumpur, Malaysia. The chimeric clone, which contains the 59 LTR (long terminal repeat) region of p93JP-NH1 (a previously isolated CRF01AE infectious clone), showed robust viral replication in the human peripheral blood mononuclear cells. This clone demonstrated robust viral propagation and profound syncytium formation in CD4(+), CXCR4-expressing human glioma NP-2 cells, indicating that p05MYKL045.1 is a CXCR4-using virus. Viral propagation, however, was not detected in various human T cell lines including MT-2, M8166, Sup-T1, H9, Jurkat, Molt-4 and PM1.p05MYKL045.1 appears to proliferate only in restricted host range, suggesting that unknown viral and/or cellular host factors may play a role in viral infectivity and replication in human T cell lines. Availability of a CRF3301B molecular clone will be useful in facilitating the development of vaccine candidates that match the HIV-1 strains circulating in Southeast Asia. Public Library of Science 2009 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4594/1/Isolation_and_Characterization_of_a_Replication-Competent_Molecular_Clone_of_an_HIV-1_Circulating_Recombinant_Form_%28CRF33_01B%29.pdf Tee, K.K. and Kusagawa, S. and Li, X.J. and Onogi, N. and Isogai, M. and Hase, S. and Uenishi, R. and Liao, H.N. and Kamarulzaman, A. and Takebe, Y. (2009) Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B). PLoS ONE, 4 (8). e6666. ISSN 1932-6203, |
| spellingShingle | R Medicine Tee, K.K. Kusagawa, S. Li, X.J. Onogi, N. Isogai, M. Hase, S. Uenishi, R. Liao, H.N. Kamarulzaman, A. Takebe, Y. Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) |
| title | Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) |
| title_full | Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) |
| title_fullStr | Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) |
| title_full_unstemmed | Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) |
| title_short | Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF3301B) |
| title_sort | isolation and characterization of a replication competent molecular clone of an hiv 1 circulating recombinant form crf3301b |
| topic | R Medicine |
| url | http://eprints.um.edu.my/4594/1/Isolation_and_Characterization_of_a_Replication-Competent_Molecular_Clone_of_an_HIV-1_Circulating_Recombinant_Form_%28CRF33_01B%29.pdf |
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